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Characterization of Malaysian Trichoderma isolates using random amplified microsatellites (RAMS)

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Abstract

Trichoderma species are commercially applied as biocontrol agents against numerous plant pathogenic fungi due to their production of antifungal metabolites, competition for nutrients and space, and mycoparasitism. However, currently the identification of Trichoderma species from throughout the world based on micro-morphological descriptions is tedious and prone to error. The correct identification of Trichoderma species is important as several traits are species-specific. The Random Amplified Microsatellites (RAMS) analysis done using five primers in this study showed different degrees of the genetic similarity among 42 isolates of this genus. The genetic similarity values were found to be in the range of 12.50–85.11% based on a total of 76 bands scored in the Trichoderma isolates. Of these 76 bands, 96.05% were polymorphic, 3.95% were monomorphic and 16% were exclusive bands. Two bands (250 bp and 200 bp) produced by primer LR-5 and one band (250 bp) by primer P1A were present in all the Trichoderma isolates collected from healthy and infected oil palm plantation soils. Cluster analysis based on UPGMA of the RAMS marker data showed that T. harzianum, T. virens and T. longibrachiatum isolates were grouped into different clades and lineages. In this study we found that although T. aureoviride isolates were morphologically different when compared to T. harzianum isolates, the UPGMA cluster analysis showed that the majority isolates of T. aureoviride (seven from nine) were closely related to the isolates of T. harzianum.

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Acknowledgments

The authors acknowledge the valuable contributions of the late Prof. Dr. Faridah Abdullah throughout this study. This paper is therefore dedicated to her memory. The authors are indebted to the Ministry of Science, Technology and Innovation Malaysia (MOSTI) for support under the Intensified Research in Priority Areas (IRPA) grant no. 01-02-04-6020 EA001 which financed this research project.

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Authors and Affiliations

  1. Biotechnology Research Institute, Universiti Malaysia Sabah, Jln UMS, 88400, Kota Kinabalu, Sabah, Malaysia

    Shafiquzzaman Siddiquee

  2. Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor D.E., Malaysia

    Umi Kalsom Yusuf

  3. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor D.E., Malaysia

    Soon Guan Tan

  4. Department of Agricultural Science, Faculty of Agriculture and Biotechnology, Universiti Sultan Zainal Abidin, Kampus Kota, 20400, Kuala Terengganu, Malaysia

    Nur Hasan Nudin Fatihah

  5. Department of Agricultural Botany, Faculty of Agriculture, Patuakhali Science and Technology University (PSTU), Patuakhali, Bangladesh

    Md Mainul Hasan

Authors
  1. Shafiquzzaman Siddiquee
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  2. Soon Guan Tan
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  3. Umi Kalsom Yusuf
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  4. Nur Hasan Nudin Fatihah
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  5. Md Mainul Hasan
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Corresponding authors

Correspondence to Shafiquzzaman Siddiquee or Soon Guan Tan.

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Siddiquee, S., Tan, S.G., Yusuf, U.K. et al. Characterization of Malaysian Trichoderma isolates using random amplified microsatellites (RAMS). Mol Biol Rep 39, 715–722 (2012). https://doi.org/10.1007/s11033-011-0790-6

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  • DOI: https://doi.org/10.1007/s11033-011-0790-6

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