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Expression analysis of five maize MAP kinase genes in response to various abiotic stresses and signal molecules

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Abstract

Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules in eukaryotes. Plant MAPK cascades are complicated networks and play vital roles in signal transduction induced by biotic and abiotic stresses. In this paper, expression patterns of MAPKs in maize roots treated with low-temperature, osmotic stresses, wounding, plant hormones and UV-C irradiation were investigated. Semi-quantitative RT-PCR reveals that the expression of MAPKs in maize roots which treated with low-temperature in light or in low light are inducible. The expression patterns of MAPKs in maize roots with treatments of CaCl2, SA, GA and wounding are approximately the same. A detailed time course experiment shows that the expression patterns of ZmSIMK are different with treatments of PEG and NaCl, respectively. These results suggest that the expression patterns of MAPKs are complicated and the signal pathways are interlaced into a network in maize roots.

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Abbreviations

ABA:

Abscisic acid

GA:

Gibberellin acid

SA:

Salicylic acid

ET:

Ethylene

UV-C:

Ultraviolet C

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Acknowledgments

This work was supported by the Grants from the Nation Natural Science Foundation of China (grant numbers: 30871457, 31071337) and the State Key Basic Research and Development Plan of China (grant number: 2009CB118500), and also was supported by the Program for Changjiang Scholars and Innovative Research Team in University (grant number: IRT0635).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, 61 Daizong street, Taian, 271018, Shandong, People’s Republic of China

    Tao Wu, Xiang-Pei Kong, Xiao-Juan Zong & De-Quan Li

  2. College of Food Sciences, Shandong Agricultural University, 61 Daizong street, Taian, 271018, Shandong, People’s Republic of China

    Da-Peng Li

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  1. Tao Wu
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Correspondence to De-Quan Li.

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Tao Wu and Xiang-Pei Kong contributed equally to this work.

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Wu, T., Kong, XP., Zong, XJ. et al. Expression analysis of five maize MAP kinase genes in response to various abiotic stresses and signal molecules. Mol Biol Rep 38, 3967–3975 (2011). https://doi.org/10.1007/s11033-010-0514-3

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  • DOI: https://doi.org/10.1007/s11033-010-0514-3

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