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Isolation and characterization of an AP2/ERF-RAV transcription factor BnaRAV-1-HY15 in Brassica napus L. HuYou15

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Abstract

Transcriptional regulation is thought to be important for stress tolerance and response of transcription factors. RAV subfamily transcription factor contains an AP2- and B3-DNA binding domain, which belongs to the AP2/ERF family. It encodes transcriptional regulators with a variety of functions involved in the developmental and physiological processes in plants. Here, a RAV-like gene, BnaRAV-1-HY15, was isolated from Brassica napus L. cv HuYou15. Sequence homology analysis revealed that the BnaRAV-1-HY15 factor belongs to the RAV subfamily of the AP2/ERF family, and it shares high identity with the AtRAV2 of Arabidopsis. Sequence and three-dimensional structural analyses revealed that BnaRAV-1-HY15 contains two distinct DNA-binding domains, one AP2 domain together with one B3 domain. The AP2 domain composed of 54 amino acids and present in N-terminal region. In addition to AP2 domain, 117 amino acids show significant sequence similarity to the B3 domain present in C-terminal region. Semi-quantitative RT-PCR analysis indicated that the BnaRAV-1-HY15 gene is induced by cold, NaCl and PEG treatments. Under ABA stress, the expression of BnaRAV-1-HY15 gene was not detected. The gene expression was also not traceable from the tissues of pod, bud, petal, leaf, stem and root of normally grown B. napus L. HuYou15 plant at the period of flowering and seed development.

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Abbreviations

TF:

Transcription factor

AP2:

APETALA2 factor

ERF:

Ethylene responsive element binding factor

DREB:

Dehydration responsive element binding factor

RAV:

Related to ABI3/VP

Hsp:

Heat shock protein

bZIP:

Basic leucine zipper

EST:

Expressed sequence tag

ABA:

Abscisic acid

References

  1. Latchman DS (1997) Transcription factors: an overview. Int J Biochem Cell Biol 29:1305–1312

    Article  PubMed  CAS  Google Scholar 

  2. Riechmann JL, Meyerowitz EM (1998) The AP2/EREBP family of plant transcription factors. Biol Chem 379:633–646

    Article  PubMed  CAS  Google Scholar 

  3. Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J (2000) Arabidopsis transcription factors: genome- wide comparative analysis among eukaryotes. Science 290:2105–2110

    Article  PubMed  CAS  Google Scholar 

  4. Yamaguchi SK, Shinozaki K (2005) Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. Trends Plant Sci 10:88–94

    Article  Google Scholar 

  5. Yamaguchi SK, Shinozaki K (2006) Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annu Rev Plant Biol 57:781–803

    Article  Google Scholar 

  6. Sakuma Y, Liu Q, Dubouzet JG, Abe H, Shinozaki K, Yamaguchi SK (2002) DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophys Res Commun 290:998–1009

    Article  PubMed  CAS  Google Scholar 

  7. Nakano T, Suzuki K, Fujimura T, Shinshi H (2006) Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol 140:411–432

    Article  PubMed  CAS  Google Scholar 

  8. Riano PDM, Ruzicic S, Dreyer I, Mueller RB (2007) PlnTFDB: An integrative plant transcription factor database. BMC Bioinformatics 8:42

    Article  Google Scholar 

  9. Zhuang J, Cai B, Peng RH, Zhu B, Jin XF, Xue Y, Gao F, Fu XY, Tian YS, Zhao W, Qiao YS, Zhang Z, Xiong AS, Yao QH (2008) Genome-wide analysis of the AP2/ERF gene family in Populus trichocarpa. Biochem Biophys Res Commun 371:468–474

    Article  PubMed  CAS  Google Scholar 

  10. Zhuang J, Peng RH, Cheng ZM, Zhang J, Cai B, Zhang Z, Gao F, Zhu B, Fu XY, Jin XF, Chen JM, Qiao YS, Xiong AS, Yao QH (2009) Genome-wide analysis of the putative AP2/ERF family genes in Vitis vinifera. Sci Hortic 123:73–81

    Article  CAS  Google Scholar 

  11. Zhang G, Chen M, Chen X, Xu Z, Guan S, Li LC, Li A, Guo J, Mao L, Ma Y (2008) Phylogeny, gene structures and expression patterns of the ERF gene family in soybean (Glycine max L.). J Exp Bot 59:4095–4107

    Article  PubMed  CAS  Google Scholar 

  12. Zhuang J, Chen JM, Yao QH, Xiong F, Sun CC, Zhou XR, Zhang J, Xiong AS (2010) Discovery and expression profile analysis of AP2/ERF family genes from Triticum aestivum. Mol Biol Rep. doi:10.1007/s11033-010-0162-7

  13. Elliott RC, Betzner AS, Huttner E, Oakes MP, Tucker WQ, Gerentes D, Perez P, Smyth DR (1996) AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth. Plant Cell 8:155–168

    Article  PubMed  CAS  Google Scholar 

  14. Dubouzet JG, Sakuma Y, Ito Y, Kasuga M, Dubouzet EG (2003) OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J 33:751–763

    Article  PubMed  CAS  Google Scholar 

  15. Gutierrez L, Van Wuytswinkel O, Castelain M, Bellini C (2007) Combined networks regulating seed maturation. Trends Plant Sci 12:294–300

    Article  PubMed  CAS  Google Scholar 

  16. Ward JM, Smith AM, Shah PK, Galanti SE, Yi H, Demianski AJ, van der Graaff E, Keller B, Neff MM (2006) A new role for the Arabidopsis AP2 transcription factor, LEAFY PETIOLE, in gibberellin-induced germination is revealed by the misexpression of a homologous gene, SOB2/DRN-LIKE. Plant Cell 18:29–39

    Article  PubMed  CAS  Google Scholar 

  17. Qin QL, Liu JG, Zhang Z, Peng RH, Xiong AS, Yao QH, Chen JM (2007) Isolation, optimization, and functional analysis of the cDNA encoding transcription factor RdreB1 in Oryza Sativa L. Mol Breed 19:329–340

    Article  CAS  Google Scholar 

  18. Kagaya Y, Ohmiya K, Hattori T (1999) RAV1, a novel DNA binding protein, binds to bipartite recognition sequence through two distinct DNA-binding domains uniquely found in higher plants. Nucleic Acids Res 27:470–478

    Article  PubMed  CAS  Google Scholar 

  19. Jones HD, Peters NCB, Holdsworth MJ (1997) Genotype and environment interact to control dormancy and differential expression of the VIVIPAROUS 1 homolog in embryos of Avena fatua. Plant J 12:911–920

    Article  PubMed  CAS  Google Scholar 

  20. Zeng Y, Raimondi N, Kermode AR (2003) Role of an ABI3 homologue in dormancy maintenance of yellow-cedar seeds and in the activation of storage protein and Em gene promoters. Plant Mol Biol 51:39–49

    Article  PubMed  CAS  Google Scholar 

  21. Suzuki M, McCarty DR (2008) Functional symmetry of the B3 network controlling seed development. Curr Opin Plant Biol 11:548–553

    Article  PubMed  CAS  Google Scholar 

  22. Cavell AC, Lydiate DJ, Parkin IA, Dean C, Trick M (1998) Collinearity between a 30-centimorgan segment of Arabidopsis thaliana chromosome 4 and duplicated regions within the Brassica napus genome. Genome 41:62–69

    PubMed  CAS  Google Scholar 

  23. Li F, Wu X, Tsang E, Cutler AJ (2005) Transcriptional profiling of imbibed Brassica napus seed. Genomics 86:718–730

    Article  PubMed  CAS  Google Scholar 

  24. Huang X, Madan A (1999) CAP3: a DNA sequence assembly program. Genome Res 9:868–877

    Article  PubMed  CAS  Google Scholar 

  25. Guo A, He K, Liu D, Bai S, Gu X (2005) DATF: a database of Arabidopsis transcription factors. Bioinformatics 21:2568–2569

    Article  PubMed  CAS  Google Scholar 

  26. Gasteiger E, Gattiker A, Hoogland C, Ivanyi I, Appel RD, Bairoch A (2003) ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res 31:3784–3788

    Article  PubMed  CAS  Google Scholar 

  27. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  PubMed  CAS  Google Scholar 

  28. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis MEGA software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  29. Prilusky J, Felder CE, Zeev-Ben-Mordehai T, Rydberg EH, Man O, Beckmann JS, Silman I, Sussman JL (2005) FoldIndex: a simple tool to predict whether a given protein sequence is intrinsically unfolded. Bioinformatics 21:3435–3438

    Article  PubMed  CAS  Google Scholar 

  30. Schwede T, Kopp J, Guex N, Peitsch MC (2003) SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res 31:3381–3385

    Article  PubMed  CAS  Google Scholar 

  31. Zhuang J, Xiong AS, Peng RH, Gao F, Zhu B, Zhang J, Fu XY, Jin XF, Chen JM, Zhang Z, Qiao YS, Yao QH (2010) Analysis of Brassica rapa ESTs: gene discovery and expression patterns of AP2/ERF family genes. Mol Biol Rep 37:2485–2492

    Article  PubMed  CAS  Google Scholar 

  32. Allen MD, Yamasaki K, Ohme TM (1998) A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA. EMBO J 17:5484–5496

    Article  PubMed  CAS  Google Scholar 

  33. Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Hayami N, Terada T, Shirouzu M, Osanai T, Tanaka A, Seki M, Shinozaki K, Yokoyama S (2004) Solution structure of the B3 DNA binding domain of the Arabidopsis cold-responsive transcription factor RAV1. Plant Cell 16:3448–3459

    Article  PubMed  CAS  Google Scholar 

  34. Chen WJ, Zhu T (2004) Networks of transcription factors with roles in environmental stress response. Trends Plant Sci 9:591–596

    Article  PubMed  CAS  Google Scholar 

  35. Gutterson N, Reuber TL (2004) Regulation of disease resistance pathways by AP2/ERF transcription factors. Curr Opin Plant Biol 7:465–471

    Article  PubMed  CAS  Google Scholar 

  36. Meyerowitz EM, Pruitt RE (1985) Arabidopsis thaliana and plant molecular genetics. Science 229:1214–1218

    Article  PubMed  CAS  Google Scholar 

  37. Zhang G, Chen M, Chen X, Xu Z, Li L, Guo J, Ma Y (2010) Isolation and characterization of a novel EAR-motif-containing gene GmERF4 from soybean (Glycine max L.). Mol Biol Rep 37:809–818

    Article  PubMed  CAS  Google Scholar 

  38. Sohn KH, Lee SC, Jung HW, Hong JK, Hwang BK (2006) Expression and functional roles of the pepper pathogen-induced transcription factor RAV1 in bacterial disease resistance, and drought and salt stress tolerance. Plant Mol Biol 61:897–915

    Article  PubMed  CAS  Google Scholar 

  39. Kagaya Y, Hattori T (2009) Arabidopsis transcription factors, RAV1 and RAV2, are regulated by touch-related stimuli in a dose-dependent and biphasic manner. Genes Genet Syst 84:95–99

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

The research was supported by Shanghai Natural Science Foundation (08ZR1417200), Shanghai Rising-Star Program and (08QH14021), International Scientific and Technological Cooperation of Shanghai-Alberta. The authors would like to thank Dr. Jeffrey Charrois and Andrea Bailey for providing the critical reading the manuscript and valuable suggestions.

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Authors and Affiliations

  1. Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China

    Jing Zhuang, Chao-Cai Sun & Xi-Rong Zhou

  2. Life Science, Alberta Innovates-Technology Futures, Vegreville, AB, T9C 1T4, Canada

    Jing Zhuang & Jian Zhang

  3. Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China

    Ai-Sheng Xiong

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  1. Jing Zhuang
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  2. Chao-Cai Sun
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Correspondence to Xi-Rong Zhou, Ai-Sheng Xiong or Jian Zhang.

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Zhuang, J., Sun, CC., Zhou, XR. et al. Isolation and characterization of an AP2/ERF-RAV transcription factor BnaRAV-1-HY15 in Brassica napus L. HuYou15. Mol Biol Rep 38, 3921–3928 (2011). https://doi.org/10.1007/s11033-010-0508-1

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