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Association between IGF-IR, m-calpain and UCP-3 gene polymorphisms and growth traits in Nanyang cattle

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Abstract

The objective of this study was to estimate the allele and genotype frequencies of the IGF-IR/TaqI, m-calpain/HhaI, and UCP-3/BglI polymorphisms and to determine associations between these polymorphisms and growth traits in Chinese indigenous cattle breeds. Genotyping was performed on 321 animals including 135 Nanyang, 80 Qinchuan, and 106 Jiaxian cattle. No significant differences in growth traits were observed between the genotypes of IGF-IR/TaqI polymorphism in Nanyang cattle. The m-calpain/HhaI, and UCP-3/BglI polymorphisms were associated with body weight, withers height, and body length of 6 months (P < 0.05 or P < 0.01), body length of 18 months (P < 0.05), and body length and heart girth of 24 months (P < 0.01) in Nanyang cattle. In addition, Nanyang cattle aged 12 months with AB genotype of m-calpain/HhaI polymorphism had higher body length and heart girth than those with BB genotype (P < 0.01). The withers height were greater (P < 0.01) in Nanyang cattle aged 12 months with genotype AB of UCP-3/BglI polymorphism than those with genotype BB. The Nanyang cattle aged 24 months with genotype AA had higher withers height than those with genotype BB (P < 0.05). In conclusion, the m-calpain/HhaI and UCP-3/BglI polymorphisms may be used as DNA markers for selection in the breeding process of Nanyang cattle.

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Acknowledgments

We thank Professor Chuzhao Lei (College of Animal Science and Technology, Northwest A & F University) and associate Professor Chunlei Zhang (College of Life Science, Xuzhou Normal University) for their contribution in the manuscript revision. We also thank the reviewers for their helpful comments. This work was supported by natural Science Foundation of Hubei Province (No. 2008CDB064), Young Talent Foundation of Hubei Provincial Education Department (No. Q20082204), Projects of Cattle and goats breeding of Hubei Key Laboratory of Animal Embryo and Molecular Breeding (2010ZD110-119), Innovative Team Program of Hubei Normal University (2008) and Talent Introduction Program of Hubei Normal University (2007F14).

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Authors and Affiliations

  1. College of Life Science, Hubei Normal University, Huangshi, Hubei, 435002, China

    Runfeng Zhang

  2. Institute of Animal Husbandry and Veterinary, Hubei Academy of Agriculture Sciences, Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Wuhan, 430209, China

    Xiaofeng Li

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  1. Runfeng Zhang
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Correspondence to Runfeng Zhang.

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Zhang, R., Li, X. Association between IGF-IR, m-calpain and UCP-3 gene polymorphisms and growth traits in Nanyang cattle. Mol Biol Rep 38, 2179–2184 (2011). https://doi.org/10.1007/s11033-010-0346-1

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  • DOI: https://doi.org/10.1007/s11033-010-0346-1

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