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Identification of Fe-excess-induced genes in rice shoots reveals a WRKY transcription factor responsive to Fe, drought and senescence

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Abstract

Fe participates in several important reactions in plant metabolism. However, Fe homeostasis in plants is not completely understood, and molecular studies on Fe-excess stress are scarce. Rice (Oryza sativa L. ssp. indica) is largely cultivated in submerged conditions, where the extremely reductive environment can lead to severe Fe overload. In this work, we used representational difference analysis (RDA) to isolate sequences up-regulated in rice shoots after exposure to Fe-excess. We isolated 24 sequences which have putative functions in distinct cellular processes, such as transcription regulation (OsWRKY80), stress response (OsGAP1, DEAD-BOX RNA helicase), proteolysis (oryzain-α, rhomboid protein), photosynthesis (chlorophyll a/b binding protein), sugar metabolism (β glucosidase) and electron transport (NADH ubiquinone oxireductase). We show that the putative WRKY transcription factor OsWRKY80 is up-regulated in rice leaves, stems and roots after Fe-excess treatment. This up-regulation is also observed after dark-induced senescence and drought stress, indicating that OsWRKY80 could be a general stress-responsive gene. To our knowledge, this is the first report of an Fe-excess-induced transcription factor in plants.

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Abbreviations

ABA:

Abscisic acid

BAP:

6-Benzylaminopurine

CAB:

Chlorophyll a/b binding protein

DP:

Differential product

LRR:

Leucine-rich repeat receptor

MES:

2,4-Morpholino-ethane sulfonic acid

PSII:

Photosystem II

RDA:

Representational difference analysis

ROS:

Reactive oxygen species

RuBisCO:

Ribulose 1,5-bisphosphate carboxylase/oxygenase

SAG:

Senescence-associated gene

SGR:

Staygreen

TF:

Transcription factor

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Acknowledgments

This research was supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil, grant 481131/2004-3 to JPF and scholarships to FKR and PKM) and HarvestPlus (Agreement number 6005-05). RAS was recipient of a scholarship from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil). The authors thank IRGA (Instituto Rio-Grandense do Arroz) for technical support.

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  1. Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, Porto Alegre, RS, 91501-970, Brazil

    Felipe Klein Ricachenevsky, Raul Antonio Sperotto & Janette Palma Fett

  2. Departamento de Botânica, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, Porto Alegre, RS, 91501-970, Brazil

    Paloma Koprovski Menguer & Janette Palma Fett

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  1. Felipe Klein Ricachenevsky
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  2. Raul Antonio Sperotto
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Correspondence to Janette Palma Fett.

Electronic supplementary material

Supplementary Table 1

Gene-specific PCR primers used for semi-quantitative and quantitative RT-PCR (DOC 39 kb)

Supplementary Table 2

Rice sequences up-regulated by Fe-excess treatment (DOC 69 kb)

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Ricachenevsky, F.K., Sperotto, R.A., Menguer, P.K. et al. Identification of Fe-excess-induced genes in rice shoots reveals a WRKY transcription factor responsive to Fe, drought and senescence. Mol Biol Rep 37, 3735–3745 (2010). https://doi.org/10.1007/s11033-010-0027-0

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