Skip to main content
SpringerLink
Log in

Abscisic acid promoted changes in the protein profiles of rice seedling by proteome analysis

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

This study investigates the influence of exogenously applied abscisic acid (ABA) on the leaves and leaf sheaths of two-week-old rice seedling at the level of the proteome. Significant differences were observed in the two-dimensional polyacrylamide gel electrophoresis protein profiles between control and ABA treated samples. Amino-acid sequence analysis of affected proteins revealed that ABA caused drastic changes in the major photosynthetic protein, ribulose 1,5-bisphosphate carboxylase/oxygenase and accumulation of certain defense/stress-related proteins. Moreover, cutting or treating leaf sheaths with jasmonic acid (JA) rapidly increased the endogenous level of ABA, suggesting a role for ABA during the defense/stress-response. Comparative study indicated a potential overlap between ABA and JA as detected at the level of the proteome. Furthermore, in vitro protein phosphorylation experiments and in-gel kinase assays also revealed considerable changes in the phosphorylation status of some proteins, and differential effects on myelin basic protein and calcium-dependent protein kinase activities by ABA treatment, which suggests involvement of kinase in the downstream signaling cascade. These results provide evidence at proteome level for the involvement of ABA in stress-response in rice seedling.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ABA:

Abscisic acid

CDPK:

Calcium-dependent protein kinase

JA:

Jasmonic acid

MRL:

Mannose-binding rice lectin

MBP:

Myelin basic protein

PR1a:

Pathogenesis-related class 1 acidic protein

RuBisCO:

Ribulose-1, 5-bisphosphate carboxylase/oxygenase

SOD:

superoxide dismutase.

References

  1. Ohkuma K, Lyon JL, Addicot FT & Smith OE (1963) Science 142: 1592–1593.

    Google Scholar 

  2. Wright STC & Hiron RWP (1969) Nature 224: 719–720.

    Google Scholar 

  3. Mittelheuser CJ & van Steveninck RFM (1969) Nature 221: 281–282.

    Google Scholar 

  4. Carrera E & Prat S (1998) Plant J. 15: 765–771.

    Google Scholar 

  5. Birkenmeier GF & Ryan CA (1998) Plant Physiol. 117: 687–693.

    Google Scholar 

  6. Hildmann T, Ebneth M, Pena-Cortes H, Sanchez-Serrano JJ, Willmitzer L & Prat S (1992) Plant Cell 4: 1157–1170.

    Article  CAS  PubMed  Google Scholar 

  7. Dickson D and Cyranoski D (2001) Nature 409: 551–551.

    Google Scholar 

  8. Kersten B, Burkle L, Kuhn EJ, Giavalisco P, Konthur Z, Lueking A, Walter G, Eickhoff H & Schneider U (2002) Plant Mol. Biol. 48: 133–141.

    Google Scholar 

  9. Koller A, Washburn MP, Lange BM, Andon NL, Deciu C, Haynes PA, Hays L, Schieltz D, Ulaszek R, Wei J, Wolters D & Yates JR 3rd (2002) Proc. Natl. Acad. Sci. USA, 99: 11969–11974.

    Google Scholar 

  10. O’Farrell PH (1975) J. Biol. Chem. 250: 4007–4021.

    Google Scholar 

  11. Gallardo K, Job C, Groot SP, Puype M, Demol H, Vandekerckhove J and Job D (2002) Plant physiol. 129: 823–837.

    Google Scholar 

  12. Komatsu S, Konishi H, Shen S & Yang G (2003) Mol. Cell. Proteomics 2: 2–10.

    Google Scholar 

  13. Wilson KA, McManus MT, Gordon ME and Jordan TW (2002) Proteomics, 1-1114-1122.

  14. Rakwal R, Agrawal GK & Yonekura M (1999) Electrophoresis 20: 3472–3478.

    Google Scholar 

  15. Rakwal R & Komatsu S (2000 Electrophoresis, 21: 2492–2500.

    Google Scholar 

  16. Rakwal R & and Komatsu S (2001) J. Plant Physiol. 158: 679–688.

    Google Scholar 

  17. Agrawal GK, Yamazaki M, Kobayashi M, Hirochika R, Miyao A & Hirochika H (2001) Plant Physiol. 125: 1248–1257.

    Google Scholar 

  18. Romeis T, Piedras P, Zhang S, Klessig DF, Hirt H & Jones JDG (1999) Plant Cell 11: 273–287.

    Article  Google Scholar 

  19. Yang G & Komatsu S (2001) Plant Cell Physiol. 41: 1243–1250.

    Google Scholar 

  20. Hoth S, Morgante M, Sanchez JP, Hanafey MK, Tingey SV and Chua NH (2002) J. Cell Sci. 115: 4891–900.

    Google Scholar 

  21. Sugihara K, Hanagata N, Dubinsky Z, Baba S & Karube I (2000) Plant Cell Physiol. 41: 1279–1285.

    Google Scholar 

  22. Zhu D and Scandalios JG (1993) Proc. Natl. Acad. Sci. USA 90: 9310–9314.

    Google Scholar 

  23. Van Loon LC and Van Strien EA (1999) Physiol. Mol. Plant Pathol. 55: 85–97.

    Google Scholar 

  24. Yagi F, Hidaka M, Minami Y & Tadera K (1996) Plant Cell Physiol. 37: 1007–1012.

    Google Scholar 

  25. Peumans WJ & Van Damme EJM (1995) Plant Physiol. 109: 347–352.

    Google Scholar 

  26. Pearson WR & Lipman DJ (1988) Proc. Natl. Acad. Sci. USA, 85: 2444–2448.

    Google Scholar 

  27. Millward TA, Zolnierowicz S & Hemmings BA (1999) Trends Biochem. Sci. 24: 186–191.

    Google Scholar 

  28. Komatsu S, Masuda T & Abe K (1996) Plant Cell Physiol. 37: 748–753.

    Google Scholar 

  29. Zhang S and Klessig DF (1997) Plant Cell 9: 809–824.

    Google Scholar 

  30. Usami S, Banno H, Ito Y, Nishihama R & Machida Y (1995) Proc. Natl. Acad. Sci. USA 92: 8660–8664.

    Google Scholar 

  31. Knetsch ML, Wang M, Snaar-Jagalska BE & Heimovaara-Dijkstra S (1996) Plant Cell 8: 1061–1067.

    Google Scholar 

  32. Botella JR, Arteca JM, Somodevilla M & Arteca RN (1996) Plant Mol. Biol. 30: 1129–1137.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Genetics Department, National Institute of Agrobiological Sciences, 305-8602, Tsukuba, Japan

    Randeep Rakwal & Setsuko Komatsu

Authors
  1. Randeep Rakwal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Setsuko Komatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Setsuko Komatsu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rakwal, R., Komatsu, S. Abscisic acid promoted changes in the protein profiles of rice seedling by proteome analysis. Mol Biol Rep 31, 217–230 (2005). https://doi.org/10.1007/s11033-005-2710-0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-005-2710-0

Key words

Search

Navigation