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Advances and Perspectives in the Application of CRISPR-Cas9 in Livestock

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Abstract

The sophistication and revolution in genome editing and manipulation have revolutionized livestock by harvesting essential biotechnological products such as drugs, proteins, and serum. It laid down areas for the large production of transgenic food, resistance against certain diseases such as mastitis, and large production of milk and leaner meat. Nowadays, the increasing demand for animal food and protein is fulfilled using genome-editing technologies. The recent genome-editing techniques have overcome the earlier methods of animal reproduction, such as cloning and artificial embryo transfer. The genome of animals now is modified using the recent alteration techniques such as ZFNs, TALENS technique, and clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR-Cas9) system. The literature was illustrated for identifying the researchers to address the advances and perspectives in the application of Cas9 in Livestock. Cas9 is considered better than the previously identified techniques in livestock because of the production of resilience against diseases, improvement of reproductive traits, and animal production to act as a model biomedical research.

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Acknowledgements

We would like to express our full acknowledgment to BioRender [https://biorender.com/] as an online tool for creating the figures in this review.

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Authors and Affiliations

  1. Department of Clinical Medicine, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, 54000, Punjab, Pakistan

    Abdul Jabbar

  2. Department of Food Science and Human Nutrition, Faculty of Bio Science, University of Veterinary and Animal Sciences, Lahore, 54000, Punjab, Pakistan

    Farheen Zulfiqar & Mahnoor Mahnoor

  3. Department of Biological Sciences, Faculty of Fisheries and Wildlife, University of Veterinary and Animal Sciences, Lahore, 54000, Punjab, Pakistan

    Nadia Mushtaq

  4. College of Earth and Environmental Sciences, University of the Punjab, Lahore, 54590, Punjab, Pakistan

    Muhammad Hamza Zaman & Anum Salah Ud din

  5. Department of Animal Breeding and Genetics, Faculty of Veterinary and Animal Science, The Islamia University, Bahawalpur, Pakistan

    Musarrat Abbas Khan

  6. Department of Animal Breeding and Genetics, University of Veterinary and Animal Sciences, Ravi Campus, Pattoki, Punjab, Pakistan

    Hafiz Ishfaq Ahmad

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  1. Abdul Jabbar
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Contributions

HIA: designed the study, Primary draft was made by FZ, M, MHZ, ASUD, MAK, and AJ. NM: designed the figures. The manuscript was revised by AJ, MAK. All authors read the manuscript and have no conflict of interest.

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Correspondence to Hafiz Ishfaq Ahmad.

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Jabbar, A., Zulfiqar, F., Mahnoor, M. et al. Advances and Perspectives in the Application of CRISPR-Cas9 in Livestock. Mol Biotechnol 63, 757–767 (2021). https://doi.org/10.1007/s12033-021-00347-2

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