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Effect of New SNP Within Bovine Prolactin Gene Enhancer Region on Expression in the Pituitary Gland

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Abstract

A new single nucleotide polymorphism was revealed using PCR–SSCP and sequencing methods within the bovine prolactin distal promoter region described as a functional enhancer. The A→G transition at position −1043 abolishes the recognition site for Hsp92II restriction endonuclease, allowing for PCR–RFLP genotyping. The application of real-time PCR revealed that the prolactin gene expression level in the pituitary was higher in cattle with the AA genotype than in those with the GG genotype. EMSA analysis, however, showed increased nuclear protein binding to the sequence variant with G, suggesting a possible inhibition event, in which the transcription factors Pit1, Oct1, and YY1 could be involved.

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Acknowledgement

This work was financially supported by UWM grant no. 0105–0804.

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Author notes

  1. T. Malewski

    Present address: Museum and Institute of Zoology, Warsaw, Poland

Authors and Affiliations

  1. Department of Animal Genetics, University of Warmia and Mazury, Olsztyn, Poland

    P. Brym, E. Wójcik, A. Ruść & S. Kamiński

  2. Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, Wólka Kosowska, Poland

    T. Malewski, R. Starzyński, K. Flisikowski & L. Zwierzchowski

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  1. P. Brym
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Correspondence to P. Brym.

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Brym, P., Malewski, T., Starzyński, R. et al. Effect of New SNP Within Bovine Prolactin Gene Enhancer Region on Expression in the Pituitary Gland. Biochem Genet 45, 743–754 (2007). https://doi.org/10.1007/s10528-007-9115-9

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  • DOI: https://doi.org/10.1007/s10528-007-9115-9

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