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Recent advances on the GAP promoter derived expression system of Pichia pastoris

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Abstract

Pichia pastoris is an efficient host for the expression and secretion of heterologous proteins and the most important feature of P. pastoris is the existence of a strong and tightly regulated promoter from the alcohol oxidase I (AOX1) gene. The AOX1 promoter (pAOX1) has been used to express foreign genes and to produce a variety of recombinant proteins in P. pastoris. However, some efforts have been made to develop new alternative promoters to pAOX1 to avoid the use of methanol. The glyceraldehyde-3-phosphate dehydrogenase promoter (pGAP) has been used for constitutive expression of many heterologous proteins. The pGAP-based expression system is more suitable for large-scale production because the hazard and cost associated with the storage and delivery of large volume of methanol are eliminated. Some important developments and features of this expression system will be summarized in this review.

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Authors and Affiliations

  1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China

    Ai-Lian Zhang, Ying-Wen Pan, Yan-Hua Tan & Fa-zhi Tu

  2. The Key Laboratory of Gene Engineering of Ministry of Education and Department of Biochemistry, Sun Yat-Sen University, Guangzhou, 510275, Guangdong, China

    Jin-Xian Luo & Tian-Yuan Zhang

  3. Hainan Provincial Institute for Drug Control, Haikou, 570216, Hainan, China

    Ce-Yi Fu

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  1. Ai-Lian Zhang
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Supported by the National High-tech R&D Program (863 program) (No.2007AA021307).

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Zhang, AL., Luo, JX., Zhang, TY. et al. Recent advances on the GAP promoter derived expression system of Pichia pastoris . Mol Biol Rep 36, 1611–1619 (2009). https://doi.org/10.1007/s11033-008-9359-4

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