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Translation elongation factor 1-α gene from Pichia pastoris: molecular cloning, sequence, and use of its promoter

  • Applied Genetics and Molecular Biotechnology
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Abstract

The gene encoding translation elongation factor 1-α from the yeast Pichia pastoris was cloned. The gene revealed an open reading frame of 1,380 bp with the potential to encode a polypeptide of 459 amino acids with a calculated mass of 50.1 kDa. The potential of the promoter (P TEF1) in P. pastoris was investigated with comparison to the glyceraldehyde-3-phosphate dehydrogenase promoter (P GAP) by using a bacterial lipase gene as a reporter gene. P TEF1 demonstrated a tighter growth-associated expression mode, improved functioning in the presence of high glucose concentrations, and promoter activities that yielded recombinant protein at levels similar to or in one case greater than P GAP. The sequence of the gene was deposited in GenBank under accession no. EF014948.

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Acknowledgement

We are grateful to Philekorea Technology (Korea) for technical assistance with the real-time quantitative PCR method.

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Authors and Affiliations

  1. Biotechnology Process Engineering Department, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-600, South Korea

    Jungoh Ahn, Jiyeon Hong, Hyeokweon Lee, Myongsoo Park, Chunsuk Kim & Hongweon Lee

  2. Biomedical Process Development Department, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-600, South Korea

    Eungyo Lee & Joonki Jung

  3. Microbial Function lab, Korean Research Institute of Bioscience and Biotechnology, Daejeon, 305-600, South Korea

    Euisung Choi

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  1. Jungoh Ahn
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  2. Jiyeon Hong
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  3. Hyeokweon Lee
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  4. Myongsoo Park
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  5. Eungyo Lee
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  6. Chunsuk Kim
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  7. Euisung Choi
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  8. Joonki Jung
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  9. Hongweon Lee
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Correspondence to Hongweon Lee.

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Ahn, J., Hong, J., Lee, H. et al. Translation elongation factor 1-α gene from Pichia pastoris: molecular cloning, sequence, and use of its promoter. Appl Microbiol Biotechnol 74, 601–608 (2007). https://doi.org/10.1007/s00253-006-0698-6

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  • DOI: https://doi.org/10.1007/s00253-006-0698-6

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