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Identification of quantitative trait loci for resistance to rice black-streaked dwarf virus disease and small brown planthopper in rice

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Abstract

Small brown planthopper (SBPH) and its transmitted rice black-streaked dwarf virus disease (RBSDVD) cause serious damage to rice (Oryza sativa L.) production. Though breeding of resistant cultivars is believed to be one of the most important strategies for RBSDVD management, few high-resistance lines have been found to date. In the present study, we identified an indica variety, 9194, that is highly resistant to RBSDVD and analyzed the quantitative trait loci (QTLs) underlying this resistance . In total, four QTLs for RBSDVD resistance, viz. qRBSDV3, qRBSDV6, qRBSDV9, and qRBSDV11, were identified. Among them, qRBSDV6, qRBSDV9, and qRBSDV11 with LOD (logarithm [base 10] of odds) scores of 4.42–4.48, 2.11–7.26, and 5.01–7.16 were repeatedly detected in 2 years, accounting for 10.3–16.7%, 8.3–35.5%, and 20.0–31.1% of the total phenotypic variation, respectively. Further, introgression of single- or multiple-resistance QTLs into a susceptible rice variety by marker-assisted selection (MAS) indicated that stacking the QTLs could progressively enhance RBSDVD resistance, suggesting that these QTLs act additively. The same population was also used for QTL mapping of SBPH resistance. Four QTLs, viz. qSBPH1, qSBPH5, qSBPH8, and qSBPH9, with LOD scores of 2.72, 2.78, 2.15, and 2.85 were detected, explaining 13.7%, 11.0%, 12.0%, and 21.0% of the phenotypic variation, respectively. The identification of RBSDVD and SBPH resistance QTLs, and the development of single and multiple genes with pyramided lines, in this study provides innovative resources for molecular breeding of resistant rice cultivars.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC) (31522039, 31471470), Natural Science Foundation of Jiangsu Province of China (BK20150026), National Key Transformation Program (2014ZX08001-001), and Jiangsu Agricultural Scientific Self-innovation Fund (No.CX[14]2030).

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Author notes

  1. Zhiguang Sun and Yuqiang Liu contributed equally to this work.

Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Zhiguang Sun, Yuqiang Liu, Shizhuo Xiao, Jinlong Hu, Gen Pan, Jun He, Tingting Xu, Jie Huang, Zeyu Qiu, Dejia Fan, Le Zhang, Linglong Liu, Ling Jiang, Xianian Cheng & Jianmin Wan

  2. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

    Huqu Zhai & Jianmin Wan

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  1. Zhiguang Sun
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  2. Yuqiang Liu
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  3. Shizhuo Xiao
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  4. Jinlong Hu
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  5. Gen Pan
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  6. Jun He
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  7. Tingting Xu
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  8. Jie Huang
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  9. Zeyu Qiu
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  11. Le Zhang
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  12. Linglong Liu
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  13. Ling Jiang
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  14. Xianian Cheng
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  15. Huqu Zhai
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  16. Jianmin Wan
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Corresponding author

Correspondence to Jianmin Wan.

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Fig. S1 Frequency distribution of RBSDVD (a) and SBPH (b) resistance of F2:3 lines derived from a cross between 9194 and SYN (PDF 358 kb)

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Sun, Z., Liu, Y., Xiao, S. et al. Identification of quantitative trait loci for resistance to rice black-streaked dwarf virus disease and small brown planthopper in rice. Mol Breeding 37, 72 (2017). https://doi.org/10.1007/s11032-017-0669-x

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