Skip to main content

Advertisement

SpringerLink
Log in

Identification and fine mapping of qRBSDV-6 MH, a major QTL for resistance to rice black-streaked dwarf virus disease

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Rice black streaked-dwarf virus (RBSDV) disease is recently expanding in southern China and poses a serious threat to rice crops. Few studies related to the genetics and breeding of RBSDV resistance have been reported. We have previously mapped a number of quantitative trait loci (QTLs) for RBSDV resistance by using a recombinant inbred line population of ‘Zhenshan 97’ (ZS97, susceptible)/‘Minghui 63’ (MH63, resistant) with natural infection data in two locations. In the present study, we confirmed the presence of a number of resistant QTLs on chromosomes 6, 7, and 9 from MH63 by using the same population in four different locations. We then focused on a major QTL, qRBSDV-6 MH, on chromosome 6 and introduced it into a highly susceptible japonica rice variety, ‘Huaidao 5’, using MH63 as the donor via marker-assisted selection, to generate seven backcross inbred lines (BILs). Natural infection and artificial inoculation-based tests revealed that all of the BILs had a significantly higher resistance to RBSDV than the recurrent parent. These results demonstrate that qRBSDV-6 MH is a stable major resistance QTL of high breeding value. We also constructed a set of chromosome segment substitution lines (CSSLs) specific to the qRBSDV-6 MH region and these used as fine mapping population. Combining the genotypes of CSSLs with the phenotypes from natural infection data in a highly RBSDV epidemic area during two different sowing seasons, we were able to precisely map qRBSDV-6 MH to the markers S18 and S23 at a physical distance of 627.6 kb on the Nipponbare reference genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Azuhata F, Uyeda I, Kimura I, Shikata E (1993) Close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses. J Gen Virol 74:1227–1232

    Article  PubMed  CAS  Google Scholar 

  • Bae SD (1994) Occurrence of small brown planthopper (Laodelphax striatellus Fallén) and incidence of rice virus disease by different seeding date in dry seeded rice. Kor J Appl Entomol 33(3):173–177

    Google Scholar 

  • Bai FW, Yan J, Qu ZC, Zhang HW, Xu J, Ye MM, Shen DL (2002) Phylogenetic analysis reveals that a dwarfing disease on different cereal crops in China is due to rice black streaked dwarf virus (BBSDV). Virus Genes 25(2):201–206

    Article  PubMed  CAS  Google Scholar 

  • Chen SX, Yu JB, Qin WS, Liu L, Gao DM, Xu J, Wang YF (1993) Studies on stunt disease of hybrid rice in Wenzhou. Virol Sin 8(4):373–378

    Google Scholar 

  • Chen SX, Wu HL, Liao XG, Lü Y-P, Shen SF, Wang OF, Jin MS (2000) The prevalent reason of rice black-streaked dwarf viral disease in the middle of Zhejiang. Zhejiang Agric Sci 6:287–289

    CAS  Google Scholar 

  • Chen SX, Hong J, Lv YP, Lei JL (2004) Study on relation between RBDV infecting stages in maize vein cell and active transmission of Laodelphax striatellus Fallen. Virol Sin 19(2):153–157

    Google Scholar 

  • Di DP, Miao HQ, Lu YG, Tian LZ (2005) Study on the method of inoculation and identification for the resistance of maize to maize rough dwarf virus. J Agric Univ Hebei 28(2):76–78

    Google Scholar 

  • Ding X, Jiang L, Zhang Y, Sun D, Zhai H, Wan J (2005) Detection and analysis of QTL for resistance to stripe disease in rice, using backcross inbred lines. Acta Agron Sin 31:1041–1046

    CAS  Google Scholar 

  • Duan CX, Zhang SX, Chen Q, Cheng ZJ, Zhai HQ, Wan JM (2007) Evaluation of rice germplasm for resistance to the small brown planthopper and analysis on resistance mechanism. Chin J Rice Sci 21(4):425–430

    CAS  Google Scholar 

  • Duan CX, Su N, Cheng ZJ, Lei CL, Wang JL, Zhai HQ, Wan JM (2010) QTL analysis for the resistance to small brown planthopper (Laodelphax striatellus Fallén) in rice using backcross inbred lines. Plant Breed 129:63–67

    Article  CAS  Google Scholar 

  • Harding RM, Burns P, Geijskes RJ, McQualter RM, Dale JL, Smith GR (2006) Molecular analysis of Fiji disease virus segments 2, 4 and 7 completes the genome sequence. Virus Genes 32(1):43–47

    Article  PubMed  CAS  Google Scholar 

  • Kajino Y (1997) Occurrence of rice black-streaked dwarf in the Kamikawa district of Hokkaido. Annu Rep Soc Plant Protect North Japan 48:50–54

    Google Scholar 

  • Li DB, Wang GC, Shen FJ (1979) Epidemological study on rice virus disease and their control in Zhejiang province. Acta Phytopathol Sin 9(2):73–87

    Google Scholar 

  • Li CB, Song JC, Jiang LJ, Yang CY, Wang QB, Wang SY (2002) Identification of RAPD markers linked to MRDV resistant genes and their applications to marker-assisted selection. Acta Agron Sin 28(4):564–568

    Google Scholar 

  • Li AH, Dai ZY, Ji HJ, Zhang XX, Li YH, Pan CH, Zhang HX, Pan XB (2008a) Preliminary analysis on resistance of rice black-streaked dwarf viral disease for germplasms with different gene-types. J Yangzhou Univ (Agric & Life Sci Edn) 29(3):18–22

    Google Scholar 

  • Li Y, Zhang Y, Zhu Z, Zhu T, Chen T, Zhao L, Wang C (2008b) Analysis of quantitative trait loci for resistance to stripe disease in Oryza sativa. Chin Bull Bot 25:701–706

    Google Scholar 

  • Li AH, Dai ZY, Ji HJ, Zhang XX, Li YH, Pan CH, Zhang HX, Pan XB (2009) Analysis on the combining ability of resistance to rice black-streaked dwarf viral disease (RBSDV) in indica hybrid rice. J Yangzhou Univ (Agric & Life Sci Edn) 30(1):73–77

    Google Scholar 

  • Lu YJ, Zhen KL (1992) A simple method for extracting rice DNA. Chin J Rice Sci 6(1):47–48

    Google Scholar 

  • Lü YP, Lei JL, Jin DD, Chen SX (2002) Detect RBSDV with RT-PCR. Acta Agric Zhejiangensis 14(2):117–119

    Google Scholar 

  • Milne RG, Lovisolo O (1977) Maize rough dwarf and related viruses. Adv Virus Res 21:267–341

    Article  PubMed  CAS  Google Scholar 

  • Pan CH, Li AH, Chen ZX, Wu LB, Dai ZY, Zhang HX, Huang NS, Chen XJ, Zhang YF, Zuo SM, Pan XB (2009) Detection of QTL for resistance to rice black-streaked dwarf viral disease. Acta Agron Sin 35(12):2213–2217

    Article  CAS  Google Scholar 

  • Qiu XP, Cheng LP, Yang J, Zhang LC, Hu Y (2010) Investigation of susceptibility of different rice varieties to rice black-streaked dwarf virus disease. Shanghai Agric Sci 4:131–133

    Google Scholar 

  • Ren SM, Hu WY (2007) The prevalent characteristics and control techniques of rice black-streaked dwarf viral diseases in Chunan. Hangzhou Agric Sci 4:7–9

    Google Scholar 

  • Ruan YL, Jiang WL, Lin RF (1981) Study on small planthopper, a kind of medium inset transmitting rice virus. Acta Entomol Sin 24(3):283–289

    Google Scholar 

  • Ruan YL, Chen SX, Lin RF (1984) Study on rice black-streaked dwarf viral disease. J Zhejiang Agric Sci 10(5):3–4

    Google Scholar 

  • Shi LY, Li XH, Xie CX, Hao ZF, Weng JF, Zhang SH, Pan GT (2011) Development of SCAR markers for resistance to MRDV. Sci Agric Sin 44(9):1763–1774

    CAS  Google Scholar 

  • Tuyen L, Liu Y, Jiang L, Wang B, Wang Q, Hanh TTT, Wan J (2012) Identification of quantitative trait loci associated with small brown planthoper (Laodelphax striatellus Fallén) resistance in rice (Oryza sativa L.). Hereditas 149:16–23

    Article  Google Scholar 

  • Wang ZH, Zhou YJ, Fan YJ, Xue BD, Wu SH, Chen ZB, Zhang WH (2001) Detection of rice black-streaked dwarf Fijivirus by RT-PCR, dot-blot by hybridization and SDS-PAGE. J Nanjing Agric Univ 24(4):24–28

    CAS  Google Scholar 

  • Wang ZH, Fang SG, Xu JL, Sun LY, Li DW, Yu JL (2003) Sequence analysis of the complete genome of rice black-streaked dwarf virus isolated from maize with rough dwarf disease. Virus Genes 27(2):163–168

    Article  PubMed  Google Scholar 

  • Wang ZH, Fang SG, Zhang ZY, Han CG, Li DW, Yu JL (2006) Development of an ID-ELISA for the detection of Rice black-streaked dwarf virus in plants. J Virol Methods 134(1–2):61–65

    Article  PubMed  CAS  Google Scholar 

  • Wang HD, Zhu ZR, Chen JP, Wang EG, Li BF (2007) Epidemics, monitoring and key control techniques of the rice black-streaked dwarf viral disease. Acta Agric Zhejiangensis 19(3):141–146

    CAS  Google Scholar 

  • Wang HD, Chen JP, Wang AG, Jiang XH, Adama MJ (2009) Studies on the epidemiology and yield losses from rice black-streaked dwarf disease in a recent epidemics in Zhejiang province, China. Plant Pathol 58:815–825

    Article  Google Scholar 

  • Wang BX, Jiang L, Chen LM, Lu BG, Wang Q, Li GQ, Fan JW, Cheng XN, Zhai HQ, Xu DY, Wan JM (2010) Screeing of rice resources against rice black-streaked dwarf virus and mapping of resistant QTL. Acta Agron Sin 36(8):1258–1264

    Article  CAS  Google Scholar 

  • Washio O, Ezuka A, Toriyama K, Sakuri Y (1968) Studies on the breeding of rice varieties resistant to stripe disease II. Genetic study on resistance to stripe disease in Japanese upland rice. Jpn J Breed 18:96–101

    Article  Google Scholar 

  • Wu AG, Zhang SC, Gu KL, Zhang RQ, Ma L (2006) Screening for resistant rice varieties in Lixiahe region. Chin Agric Tech Exten 4:18–19

    Google Scholar 

  • Xing YZ, Tan YF, Xu CG, Hua JP, Sun XL (2001) Mapping quantitative trait loci for grain appearance traits of rice using a recombinant inbred line population. Acta Bot Sin 43(8):840–845

    CAS  Google Scholar 

  • Zhang HM, Chen JP, Lei JL, Adams MJ (2001a) Sequence analysis shows that a dwarf disease on rice, wheat and maize in China is caused by Rice black-steaked dwarf virus. Eur J Plant Pathol 1107:563–567

    Article  Google Scholar 

  • Zhang HM, Chen JP, Adams MJ (2001b) Molecular characterization of segments 1 to 6 of Rice black-streaked dwarf virus from China provides the complete genome. Arch Virol 146:2331–2339

    Article  PubMed  CAS  Google Scholar 

  • Zhang HM, Yang J, Chen JP, Adams MJ (2008) A black-streaked dwarf disease on rice in China is caused by a novel fijivirus. Arch Virol 153(10):1893–1898

    Article  PubMed  CAS  Google Scholar 

  • Zhou T, Wu LJ, Wang Y, Chen ZB, Ji YH, Fan YJ, Zhou YJ (2010) Preliminary report on the transmission of rice black-streaked dwarf virus from frozen infected leaves to rice by insect vector small brown planthopper (Laodelphax striatellus). Chin J Rice Sci 4:425–428

    Google Scholar 

  • Zhu ZR, Wu HL, Liao XG (1998) Prevalence and control of rice black-streaked dwarf viral disease transmitted by small planthopper. In: Chen JA, Zhou WJ (eds) Study on agricultural development in new century. China Environmental Science Press, Beijing, pp 458–464

    Google Scholar 

  • Zhu ZR, Wu HL, Liao XG (2005) A stirring among the dry bones or smoke fly ashes? In: Cheng ZM (ed) Prevention and control of agricultural biological disasters. China Agricultural Science Press, Beijing, pp 356–363

    Google Scholar 

Download references

Acknowledgments

We thank Professor Yongzhong Xing of Huazhong Agricultural University for supplying the RILs of ZS97/MH63. We appreciate the help of Professor Dayong Xu of Lianyungang Academy of Agricultural Sciences and Professor Dayou Zhang of Yandu County Agricultural Research Institute in the NI-based evaluation. This study was supported by the National Natural Science Foundation (30900886) of P. R. China, the Natural Science Foundation (BK2011428) of Jiangsu Province, the Innovation Fund [CX(11)4001] of the Jiangsu Academy of Agricultural Science and Technology, the 333 Project Research Funding (BRA2011080) of Jiangsu Province, the Six Talent-Peak Project of Jiangsu Province, and the Scientific and Technological Project (BE2012303) of Jiangsu Province.

Author information

Authors and Affiliations

  1. Lixiahe Agricultural Research Institute, Yangzhou, 225007, Jiangsu Province, People’s Republic of China

    Aihong Li, Cunhong Pan, Zhengyuan Dai, Nin Xiao, Ling Yu, Yuhong Li, Xiaoxiang Zhang & Hongxi Zhang

  2. Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou, 225009, People’s Republic of China

    Linbo Wu, Shimin Zuo, Wenxia Xue & Xuebiao Pan

Authors
  1. Aihong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cunhong Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Linbo Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhengyuan Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shimin Zuo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Nin Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ling Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yuhong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Xiaoxiang Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Wenxia Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Hongxi Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Xuebiao Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Aihong Li or Xuebiao Pan.

Additional information

Aihong Li and Cunhong Pan contributed equally to this study.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 4325 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, A., Pan, C., Wu, L. et al. Identification and fine mapping of qRBSDV-6 MH, a major QTL for resistance to rice black-streaked dwarf virus disease. Mol Breeding 32, 1–13 (2013). https://doi.org/10.1007/s11032-012-9807-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-012-9807-7

Keywords

Search

Navigation