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Mapping QTLs conferring resistance to rice black-streaked dwarf disease in rice (Oryza sativa L)

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Abstract

Rice black-streaked dwarf disease, caused by the rice black-streaked dwarf virus (RBSDV), can lead to severe yield losses in rice. The deployment of resistant cultivars is an effective disease control measure, but few studies related to the genetics and breeding of RBSDV resistance have been reported in rice. Here, we identified ‘IR36’ (indica) and ‘L5494’ (japonica) as resistant and susceptible parents, respectively, using a field test, and 208 recombinant inbred lines were derived from their cross. A genetic analysis indicated that the resistance of rice materials to RBSDV was controlled by quantitative trait loci (QTLs). A total of 12 QTLs for RBSDV resistance on chromosomes 1, 6, 8 and 9 were identified in three environments (2013–2015), and QTLs in two marker intervals, RM19234–CHR6-2 and RM3700–RM160 on chromosomes 6 and 9, respectively, were consistently detected. These QTLs explained 6.19–29.00 % of the total phenotypic variation for rice black-streaked dwarf disease incidence. The alleles enhancing resistance on chromosomes 6 and 8 originated from ‘IR36’, whereas the alleles on chromosomes 1 and 9 originated from ‘L5494’. The materials and identified resistance QTLs in this study are expected to be useful resources for efficiently breeding rice cultivars resistant to RBSDV.

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Acknowledgments

This study was financially supported by the National Program on Research and Development of Transgenic Plants of China (2014ZX08001002-003, Ministry of Agriculture of PR China); the Science and Technology Support Program of Jiangsu Province (BE2013301); the National Basic Research Program of China (2013CBA01405); and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Author contributions

HZ conducted data analyses and drafted the manuscript. YG, MW and JL performed the phenotypic evaluations and genotypic identifications. LZ and HS participated in the construction of the populations. GL and MG participated in the design of the study. ST designed this study and revised the manuscript. All of the authors have read and approved the final manuscript.

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    Authors and Affiliations

    1. Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou, 225009, China

      Honggen Zhang, Yongshen Ge, Maoyu Wang, Jiangning Liu, Hua Si, Lijia Zhang, Guohua Liang, Minghong Gu & Shuzhu Tang

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    1. Honggen Zhang
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    2. Yongshen Ge
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    5. Hua Si
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    Correspondence to Shuzhu Tang.

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    Honggen Zhang, and Yongshen Ge contributed have contributed equally to this paper.

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    Zhang, H., Ge, Y., Wang, M. et al. Mapping QTLs conferring resistance to rice black-streaked dwarf disease in rice (Oryza sativa L). Euphytica 212, 323–330 (2016). https://doi.org/10.1007/s10681-016-1782-3

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