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QTL mapping of panicle blast resistance in japonica landrace heikezijing and its application in rice breeding

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Abstract

The rice blast caused by Magnaporthe oryzae is one of the most devastating diseases worldwide, and the panicle blast could result in more loss of yield in rice production. However, the quantitative trait loci (QTLs) and genes related to panicle-blast resistance have not been well studied due to the time-consuming screening methodology involved and variation in symptoms. The QTLs for panicle blast resistance have been mapped in a population of 162 RILs (recombination inbreeding lines), derived from a cross between a highly blast-resistant rice landrace, Heikezijing, and a susceptible variety, Suyunuo. Two QTLs for panicle-blast resistance, qPbh-11–1 and qPbh-7-1, were identified, which were distributed on chromosomes 11 and 7. The QTL qPbh-11–1 was stably detected in three independent experiments, at Nanjing in 2013 and 2014 and at Hainan in 2014, located between the region of RM27187 and RM27381 on the distal end of chromosome 11 far from the reported resistant loci Pb1 and qPbm11 for panicle blast. The QTL qPbh-7-1 was detected only at Nanjing in 2013 and located between the region of M18 and RM3555 on chromosome 7. With marker-assisted selection (MAS) three introgression lines with the major panicle blast-resistance QTL qPbh-11–1 were developed from a recurrent parent Nanjing 44 (NJ44) and the panicle resistance of introgression lines was improved 46.36–55.47 % more than NJ44. Based on the results provided, Heikezijing appears to be a valuable source for panicle blast resistance.

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Acknowledgements

This research was supported by grants from the National Key Project for Transgenic Crops (2016ZX08009-003-001, 2014ZX08009-001B), the Natural Science Foundation of China (31171516, 30900888) and the Fundamental Research Funds for the Central Universities (KYZ201302), Jiangsu Agriculture Science and Technology Innovation Fund (CX(12)1003-3, CX(15)1054). We would like to thank Prof. Chen Zhi-Yi and Dr. Liu Yong-Feng for kindly providing all the blast isolates.

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Authors and Affiliations

  1. State Key Laboratory of crop Genetics & Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China

    Nengyan Fang, Ruisen Wang, Wanwan He, Congfei Yin, Changhong Guan, Hao Chen, Ji Huang, Jianfei Wang, Yongmei Bao & Hongsheng Zhang

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  1. Nengyan Fang
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  2. Ruisen Wang
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  3. Wanwan He
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  4. Congfei Yin
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  5. Changhong Guan
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  6. Hao Chen
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  7. Ji Huang
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  9. Yongmei Bao
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  10. Hongsheng Zhang
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Correspondence to Yongmei Bao or Hongsheng Zhang.

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Fang, N., Wang, R., He, W. et al. QTL mapping of panicle blast resistance in japonica landrace heikezijing and its application in rice breeding. Mol Breeding 36, 171 (2016). https://doi.org/10.1007/s11032-016-0603-7

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