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Genetics and fine mapping of a yellow-green leaf gene (ygl-1) in cabbage (Brassica oleracea var. capitata L.)

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Abstract

Cabbage (Brassica oleracea var. capitata L.) is one of the most popular cultivated vegetables worldwide. Cabbage has rich phenotypic diversity, including plant height, head shape, head color, leaf shape and leaf color. Leaf color plays an important role in cabbage growth and development. At present, there are few reports on fine mapping of leaf color mutants in B. oleracea. In this study, a naturally occurring yellow-green leaf cabbage mutant (YL-1), derived from the self-pollinated progenies of the hybrid ‘Hosom’, was used for inheritance analysis and gene mapping. Segregation populations including F2 and BC1 were generated from the cross of two inbred lines, YL-1 and 01–20. Genetic analysis with the F2 and BC1 populations demonstrated that the yellow-green leaf color was controlled by a single recessive nuclear gene, ygl-1. Insertion–deletion (InDel) markers, designed based on the parental re-sequencing data, were used for the preliminary mapping with BSA (bulked segregant analysis) method. A genetic map constructed with 15 InDels indicated that ygl-1 was located on chromosome C01. The ygl-1 gene is flanked by InDel markers ID2 and M8, with genetic distances of 0.4 cM and 0.35 cM, respectively. The interval distance between two markers is 167 kb. Thus, it enables us to locate the ygl-1 gene for the first time in B. oleracea. This study lays the foundation for candidate gene prediction and ygl-1gene cloning.

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Acknowledgments

This work was financially supported by grants from the National Natural Science Foundation of China (31272180, 31572141), the Major State Basic Research Development Program (973 Program, 2012CB113906), the Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS) and the earmarked fund for the Modern Agro-Industry Technology Research System, China (nycytx-35-gw01). This work was performed in the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Beijing 100081, People’s Republic of China.

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    Authors and Affiliations

    1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 12# ZhongGuanCun Nandajie, Beijing, 100081, People’s Republic of China

      Xiao-ping Liu, Chong Yang, Feng-qing Han, Zhi-yuan Fang, Li-mei Yang, Mu Zhuang, Hong-hao Lv, Yu-mei Liu, Zhan-sheng Li & Yang-yong Zhang

    2. National Engineering Research Center for Agriculture in Northern Mountainous Areas, Agricultural University of Hebei, 2596# LeKai Nandajie, Baoding, 071001, Hebei, People’s Republic of China

      Chong Yang

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    1. Xiao-ping Liu
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    Correspondence to Yang-yong Zhang.

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    Xiao-ping Liu and Chong Yang have contributed equally to this work.

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    Liu, Xp., Yang, C., Han, Fq. et al. Genetics and fine mapping of a yellow-green leaf gene (ygl-1) in cabbage (Brassica oleracea var. capitata L.). Mol Breeding 36, 82 (2016). https://doi.org/10.1007/s11032-016-0509-4

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