Abstract
Cabbage Fusarium wilt (CFW) is a destructive disease causing great losses to cabbage (Brassica oleracea L. var. capitata L.) production worldwide. At present, there are few reports concerning molecular marker research on cabbage resistance to CFW. In this study, 160 double haploid (DH) lines were obtained from the F1 population of a 99–77 (highly resistant to CFW) × 99–91 (highly susceptible to CFW) cross. Insertion–deletion (InDel) markers were designed according to the reference genome sequence of cabbage and the whole-genome re-sequencing data of the two parents. A genetic map of chromosome C06 including seven InDel markers was constructed based on the DH population. Thus, FOC (resistance gene to Fusarium oxysporum f. sp. conglutinans) was located on chromosome C06 and two InDel markers out of the seven, M10 and A1, flanked the gene at 1.2 and 0.6 cM, respectively. Marker A1 revealed a significant consistency with the phenotype assay in the F2 population as well as in 40 inbred lines (96 and 82 %, respectively). This study lays the foundation for fine mapping and cloning of the FOC gene and for marker-assisted selection in cabbage resistance breeding.
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Acknowledgments
This work has been financed as follows: Agro-scientific Research in the Public Interest (200903049-04), National ‘twelfth five’ Science and Technique Support Project (2012BAD19B00), the National Natural Science Foundation of China (31171958), the Program of Beijing Municipal Science and Technology Committee (D121100003412001), National High Technology Research and Development Program of China (863 Program, 2012AA100101; 2012AA100103), Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-Year Plan Period (2012BAD02B01), and the earmarked fund for the Modern Agro-Industry Technology Research System, China (nycytx-35-gw01). This work was done in the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing 100097, People’s Republic of China.
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Lv, Hh., Yang, Lm., Kang, Jg. et al. Development of InDel markers linked to Fusarium wilt resistance in cabbage. Mol Breeding 32, 961–967 (2013). https://doi.org/10.1007/s11032-013-9925-x
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DOI: https://doi.org/10.1007/s11032-013-9925-x