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Fine mapping of the temperature-sensitive semi-dwarf (Tssd) locus regulating the internode length in peach (Prunus persica)

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Abstract

As a key component of tree architecture, plant height is an important agronomic trait in fruit trees. Reducing tree height is beneficial for increasing planting density, allowing higher yields, lowering costs and increasing the orchard’s lifespan. However, the genetic and molecular factors that regulate peach height are unknown. Here, we report a semi-dwarf peach mutant, which exhibits extremely shortened internodes at temperature below 30 °C. Genetic analysis indicated that this trait was controlled by a single dominant gene that we have named as temperature-sensitive semi-dwarf (Tssd). To map the Tssd locus, two DNA pools, each consisting of 50 mutant or wild-type siblings, were subjected to specific length amplified fragment sequencing. SLAF analysis followed by marker validation using Sanger sequencing and high-resolution melting genotyping located the Tssd gene in a region spanning approximately 750 kb between 2.35 and 3.10 Mb in scaffold 3 based on the reference peach genome sequence of ‘Lovell.’ Further SNPs were developed for fine mapping the locus, which spans an interval of 500 Kb and contains 69 predicted protein-coding gene models. The SNP markers flanking this interval can be applied in molecular identification of Tssd individuals. The result will provide information on how temperature regulates internode length in temperate fruit trees.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (31500558, 31470679), the Agricultural Science and Technology Innovation Program (ASTIP) (CAAS-ASTIP-2016-ZFRI) and National High-tech R&D Program of China (2011AA10020606). Also we thank Nian Wang from Oilcrop Research Institute, Chinese Academy of Agricultural Sciences, for help in data processing.

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Authors and Affiliations

  1. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture, Zhengzhou, 450009, China

    Zhenhua Lu, Liang Niu, Guochao Cui, Lei Pan, Ruiping Zhang, Wenfang Zeng & Zhiqiang Wang

  2. The New Zealand Institute for Plant and Food Research Limited (Plant & Food Research), Palmerston North Research Centre, Palmerston North, New Zealand

    David Chagné & Toshi Foster

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  1. Zhenhua Lu
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  2. Liang Niu
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  3. David Chagné
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  4. Guochao Cui
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  5. Lei Pan
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  7. Ruiping Zhang
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Correspondence to Zhiqiang Wang.

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Lu, Z., Niu, L., Chagné, D. et al. Fine mapping of the temperature-sensitive semi-dwarf (Tssd) locus regulating the internode length in peach (Prunus persica). Mol Breeding 36, 20 (2016). https://doi.org/10.1007/s11032-016-0442-6

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