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SNP markers linked to leaf rust and grain mold resistance in sorghum

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Abstract

Grain mold and rust are diseases that can significantly reduce sorghum grain yield. Breeding for resistance to these diseases is hindered by inefficient disease screening. A viable option to greatly improve breeding efficiency is to identify molecular markers or genes linked to the host resistance. In this study, we applied 14,739 single nucleotide polymorphism markers to the sorghum mini core of 242 accessions that had been evaluated for rust resistance in both greenhouse and field and for grain mold in the field for 2 years. Through association mapping we have identified two loci linked to grain mold resistance and five loci linked to rust resistance. Among the two loci linked to grain mold resistance, one contained a homolog of the maize nonhost resistance gene Rxo1. Two of rust-linked loci each contained the rust resistance gene homologous to the maize rust resistance gene Rp1-D which is the B locus (the A locus containing Pu was not linked in this study) and to the wheat rust resistance gene Lr1. The remaining loci contained genes important in other steps of the defense response, such as cyclophilins that mediate resistance response preceding hypersensitive response (HR) and Hin1 directly involved in producing HR. The results from this study will facilitate marker-assisted selection of host resistance to grain mold and rust in sorghum.

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Acknowledgments

This study was supported in part by the International Crops Research Institute for the Semi-Arid Tropics and the University of Louisiana at Lafayette. We thank the reviewer for suggestions that improved the manuscript.

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Authors and Affiliations

  1. International Crops Research Institute for the Semi Arid Tropics (ICRISAT), Patancheru, 502 324, Andhra Pradesh, India

    Hari D. Upadhyaya, Rajan Sharma & Shivali Sharma

  2. Department of Biology, University of Louisiana at Lafayette, Lafayette, LA, 70504, USA

    Yi-Hong Wang

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  1. Hari D. Upadhyaya
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  3. Rajan Sharma
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Correspondence to Yi-Hong Wang.

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Upadhyaya, H.D., Wang, YH., Sharma, R. et al. SNP markers linked to leaf rust and grain mold resistance in sorghum. Mol Breeding 32, 451–462 (2013). https://doi.org/10.1007/s11032-013-9883-3

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