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Genetic variation underlying pod size and color traits of common bean depends on quantitative trait loci with epistatic effects

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Abstract

Common bean is an important vegetable legume in many regions of the world. Size and color of fresh pods are the key factors for deciding the commercial acceptance of bean as a fresh vegetable. The genetic basis of important horticultural traits of common bean is still poorly understood, which hinders DNA marker-assisted breeding in this crop. Here we report the identification of single-locus and epistatic quantitative trait loci (QTLs), as well as their environment interaction effects for six pod traits, namely width, thickness, length, size index, beak length and color, using an Andean intra-gene pool recombinant inbred line population from a cross between a cultivated common bean and an exotic nuña bean. The QTL analyses performed detected a total of 23 QTLs (single-locus QTLs and epistatic QTLs): five with only individual additive effects and six with only epistatic effects, while the remaining twelve showed both effects. These QTLs were distributed across linkage groups (LGs) 1, 2, 4, 6, 7, 8, 9, 10 and 11; particularly noteworthy are the QTLs for pod size co-located on LGs 1 and 4, indicative of tight linkage or genes with pleiotropic effects governing these traits. Overall, the results obtained showed that additive and epistatic effects are the major genetic basis of pod size and color traits. The mapping of QTLs including epistatic loci for the six pod traits evaluated provides support for implementing marker-assisted selection toward genetic improvement of common bean.

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Acknowledgments

This work was financed by the Ministerio de Economía y Competitividad and UE-FEDER Program (AGL2011-25562). We thank Junta de Andalucía (Grant Number P10-AGR-06931) and Campus de Excelencia Internacional Agroalimentario-CeiA3 for partially supporting this work.

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Authors and Affiliations

  1. Departamento de Biología y Geología (Genética), Edificio CITE II-B, Centro de Investigación en Biotecnología Agroalimentaria (BITAL), Universidad de Almería, Carretera de Sacramento s/n, 04120, Almería, Spain

    Fernando J. Yuste-Lisbona, Carmen Capel, Manuel García-Alcázar, Juan Capel & Rafael Lozano

  2. Departamento de Recursos Fitogenéticos, Grupo de Biología de Agrosistemas, Misión Biológica de Galicia, CSIC, P.O. Box 28, 36080, Pontevedra, Spain

    Ana M. González, Antonio M. De Ron & Marta Santalla

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  1. Fernando J. Yuste-Lisbona
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  2. Ana M. González
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  3. Carmen Capel
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  4. Manuel García-Alcázar
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  5. Juan Capel
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  6. Antonio M. De Ron
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  7. Marta Santalla
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  8. Rafael Lozano
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Correspondence to Rafael Lozano.

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Fernando J. Yuste-Lisbona and Ana M. González have contributed equally to this work.

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Yuste-Lisbona, F.J., González, A.M., Capel, C. et al. Genetic variation underlying pod size and color traits of common bean depends on quantitative trait loci with epistatic effects. Mol Breeding 33, 939–952 (2014). https://doi.org/10.1007/s11032-013-0008-9

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