Skip to main content

Advertisement

SpringerLink
Log in

Mapping of QTLs for morpho-agronomic and seed quality traits in a RIL population of common bean (Phaseolus vulgaris L.)

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The objective of this research was to determine the quantitative trait loci (QTLs) controlling phenological traits (days to flowering, days to end of flowering, days to harvest as green pod, and days to maturity), seed size traits (seed length, seed height, seed width, and seed weight), and seed quality traits (water absorption, and coat proportion), in common bean. A population of 104 F7 recombinant inbred lines (RILs) derived from an inter-gene pool cross between Xana, and Cornell 49242, was used to develop a genetic linkage map including 175 AFLPs, 27 microsatellites, 30 SCARs, 33 ISSRs, 12 RAPDs, 13 loci codifying for seed proteins, and the four genes Fin,fin (growth habit); Asp,asp (seed coat shininess); P,p (seed color); and I,i (resistance to bean common mosaic virus). The map has a total length of 1,042 cM distributed across 11 linkage groups aligned to those of the core linkage map of bean using common molecular markers as anchor points. The QTL analyses were carried out over three environments using the mean environment data with composite interval mapping. Thirty-one QTLs for ten traits were found to be significant in at least one environment and in the mean environment data, the number of significant QTLs identified per trait ranging from two to five. Twenty-seven of these QTLs mapped forming clusters in eight different chromosomal regions. The rationale for this clustered mapping and the possible relationship between some QTLs for phenological traits and the genes Fin and I are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Aastveit AH, Aastveit K (1993) Effects of genotype-environment interactions on genetic correlations. Theor Appl Genet 86:1007–1013

    Article  Google Scholar 

  • Adam-Blondon AF, Sévignac M, Bannerot H, Dron M (1994) SCAR, RAPD and RFLP markers linked to a dominant gene (Are) conferring resistance to anthracnose. Theor Appl Genet 88:865–870

    Article  CAS  Google Scholar 

  • Aramuganathan K, Earle DE (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218

    Article  Google Scholar 

  • Beattie AD, Larsen J, Michaels TE, Pauls KP (2003) Mapping quantitative trait loci for a common bean (Phaseolus vulgaris L.) ideotype. Genome 46:411–422

    Article  PubMed  CAS  Google Scholar 

  • Blair MW, Pedraza F, Buendía HF, Gaitán-Solís E, Beebe SE, Gepts P, Tohme J (2003) Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theor Appl Genet 107:1362–1374

    Article  PubMed  CAS  Google Scholar 

  • Blair MW, Iriarte G, Beebe S (2006) QTL analysis of yield traits in an advanced backcross population derived from a cultivated Andean wild common bean (Phaseolus vulgaris L.) cross. Theor Appl Genet 112:1149–1163

    Article  PubMed  CAS  Google Scholar 

  • Blair MW, Buendía HF, Giraldo MC, Métais I, Peltier D (2008) Characterization of AT-rich microsatellites in common bean (Phaseolus vulgaris L.). Theor Appl Genet 118:91–103

    Article  PubMed  CAS  Google Scholar 

  • Broughton WJ, Hernández G, Blair M, Beebe S, Gepts P, Vanderleyden J (2003) Bean (Phaseolus spp.); model food legumes. Plant Soil 252:55–128

    Article  CAS  Google Scholar 

  • Castellanos JZ, Guzmán-Maldonado H, Acosta-Gallegos JA, Kelly JD (1995) Effects of hardshell character on cooking time of common beans grown in the semiarid highlands of Mexico. J Sci Food Agr 69:437–443

    Article  CAS  Google Scholar 

  • Checa OE, Blair MW (2008) Mapping QTL climbing ability and component traits in common bean (Phaseolus vulgaris L.). Mol Breed 22:201–215

    Article  CAS  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Conti L (1985) Conclusive results of a selection programme for obtaining a dwarf bean (Ph. Vulgaris) resistant to some viruses and characterized by agronomical qualities. Genet Agr 39:51–63

    Google Scholar 

  • Elia FM, Hosfield GL, Kelly JD, Uebersax MA (1997) Genetic analysis and interrelationships between traits for cooking time, water absorption, and protein and tannin content of Andean dry beans. J Am Soc Hort Sci 122(4):512–518

    Google Scholar 

  • Ferreira JJ, Álvarez E, Fueyo MA, Roca A, Giraldez R (2000) Determination of the outcrossing rate of Phaseolus vulgaris L. using seed protein markers. Euphytica 113:259–263

    Article  Google Scholar 

  • Freyre R, Skroch PW, Geffroy V, Adam-Blondon AF, Shirmohamadali A, Johnson WC, Llaca V, Nodari RO, Pereira PA, Tsai SM, Tohme J, Dron M, Nienhuis J, Vallejos CE, Gepts P (1998) Towards an integrated linkage map of common bean. 4. Development of a core linkage map and alignment of RFLP maps. Theor Appl Genet 97:847–856

    Article  CAS  Google Scholar 

  • Gaitán-Solís E, Duque MC, Edwards KJ, Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris L.): Isolation, characterization, and cross-species amplification in Phaseolus spp. Crop Sci 42:2128–2136

    Google Scholar 

  • Geffroy V, Creusot F, Falquet J, Sévignac M, Adam-Blondon AF, Bannerot H, Gepts P, Dron M (1998) A family of LRR sequences in the vicinity of the Co-2 locus for anthracnose resistance in Phaseolus vulgaris and its potential use in marker-assisted selection. Theor Appl Genet 96:494–502

    Article  CAS  Google Scholar 

  • Gepts P (1998) Origin and evolution of common bean: past events and recent trends. HortScience 33:1124–1130

    Google Scholar 

  • Gepts P, Osborne TC, Rashka K, Bliss FA (1986) Phaseolin protein variability in wild forms and landraces of the common bean (Phaseolus vulgaris L.): Evidence for multiple centers of domestication. Econ Bot 40:451–468

    CAS  Google Scholar 

  • Grisi MCM, Blair MW, Gepts P, Brondani C, Pereira PAA, Brondani RPV (2007) Genetic mapping of a new set of microsatellite markers in a reference common bean (Phaseolus vulgaris L.) population BAT93 × Jalo EEP558. Genet Mol Res 6(3):691–706

    PubMed  CAS  Google Scholar 

  • Kelly JD, Gepts P, Miklas PN, Coyne DP (2003) Tagging and mapping of genes and QTL and molecular-marker assisted section for traits of economic importance in bean and cowpea. Field Crops Res 82:135–154

    Article  Google Scholar 

  • Koinange EMK, Singh SP, Gepts P (1996) Genetic control of the domestication syndrome in common bean. Crop Sci 36:1037–1045

    Article  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  • Miklas PN, Stone V, Daly MJ, Stavely JR, Steadman JR, Bassett MJ, Delorme R, Beaver JS (2000) Bacterial, fungal, and viral disease resistance loci mapped in a recombinant inbred common bean population (‘Dorado’/XAN 176). J Am Soc Hort Sci 125:476–481

    CAS  Google Scholar 

  • Miklas PN, Pastor-Corrales MA, Jung G, Coyne DP, Kelly JD, McClean PE, Gepts P (2002) Comprehensive linkage map of bean rust resistance genes. Annu Rep Bean Improv Coop 45:125–129

    Google Scholar 

  • Murray JD, Larsen J, Michaels TE, Schaafsma A, Vallejos CE, Pauls KP (2002) Identification of putative genes in bean (Phaseolus vulgaris) genomic (Bng) RFLP clones and their conversion to WISs. Genome 45:1013–1024

    Article  PubMed  CAS  Google Scholar 

  • Nienhuis J, Singh SP (1988) Genetics of seed yield and its components in common bean (Phaseolus vulgaris L.) of Middle-American origin. Plant Breeding 101:143–154

    Article  Google Scholar 

  • Nodari RO, Tsai SM, Gilbertson RL, Gepts P (1993) Towards an integrated linkage map of common bean II. Development of an RFLP based linkage map. Theor Appl Genet 85:513–520

    Article  CAS  Google Scholar 

  • Pañeda A, Rodríguez-Suárez C, Ferreira JJ, Giraldez R (2005) Molecular characterization and phylogeny of thirty common bean varieties. Annu Rep Bean Improv Coop 48:24–25

    Google Scholar 

  • Pañeda A, Rodríguez-Suárez C, Campa A, Ferreira JJ, Giraldez R (2008) Molecular markers linked to the fin gene controlling determinate growth habit in common bean. Euphytica 162:241–248

    Article  CAS  Google Scholar 

  • Park SO, Coyne DP, Jung G, Skroch PW, Arnaud-Santana E, Steadman JR, Ariyarathne HM, Nienhuis J (2000) Mapping of QTL for seed size and shape traits in common bean. J Am Soc Hort Sci 125:466–475

    CAS  Google Scholar 

  • Park SO, Crosby KM, Coyne DP, Steadman JR (2003) Development of a SCAR marker linked to the Ur-6 gene for specific rust resistance in common bean. Annu Rep Bean Improv Coop 46:189–190

    Google Scholar 

  • Park SO, Coyne DP, Steadman JR, Crosby KM, Brick MA (2004) RAPD and SCAR markers linked to the Ur-6 Andean gene controlling specific rust resistance in common bean. Crop Sci 44:1799–1807

    CAS  Google Scholar 

  • Polignano GB (1983) Heritable variation of some agronomic traits in Phaseolus vulgaris L. Genet Agr 37:69–82

    Google Scholar 

  • Rodríguez-Suárez C, Méndez-Vigo B, Pañeda A, Ferreira JJ, Giraldez R (2007) A genetic linkage map of Phaseolus vulgaris L. and localization of genes for specific resistance to six races of anthracnose (Colletotrichum lindemuthianum). Theor Appl Genet 114:713–722

    Article  PubMed  CAS  Google Scholar 

  • Singh SP (1982) A key for identification of different growth habits of Phaseolus vulgaris L. Annu Rep Bean Improv Coop 25:92–94

    Google Scholar 

  • Singh SP, Teran H, Molina A, Gutiérrez JA (1991) Genetics of seed yield and its components in common beans (Phaseolus vulgaris L.) of Andean origin. Plant Breed 107(3):254–257

    Article  Google Scholar 

  • Tanksley SD (1993) Mapping polygenes. Annu Rev Genet 27:205–233

    Article  PubMed  CAS  Google Scholar 

  • Tar’an B, Michaels TE, Pauls KP (2002) Genetic mapping of agronomic traits in common bean. Crop Sci 42:544–556

    Article  Google Scholar 

  • Tsai SM, Nodari RO, Moon DH, Camargo LEA, Vencovsky R, Geps P (1998) QTL mapping for nodule number and common bacterial blight in Phaseolus vulgaris L. Plant Soil 204:135–145

    Article  CAS  Google Scholar 

  • Vallejos CE, Sakiyama NS, Chase CD (1992) A molecular marker-based linkage map of Phaseolus vulgaris L. Genetics 131:733–740

    PubMed  CAS  Google Scholar 

  • van Ooijen JW, Voorrips RE (2001) Join map® 3.0. Software for the calculation of genetic linkage maps. Plant Research International. Wageningen, The Netherlands

    Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Lee TVD, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Wang S, Basten CJ, Zeng ZB (2005) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh

    Google Scholar 

  • Yu K, Park SJ, Poysa V, Gepts P (2000) Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.). J Hered 91:429–434

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants AGL2001-2676-C02 and AGL2007-66563-CO2-02 from the Ministerio de Ciencia y Tecnología, Spain. Elena Pérez-Vega and Ana Campa were recipient of salaries fellowship from the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA, Spain).

Author information

Authors and Affiliations

  1. Área de Cultivos Hortofrutícolas y Forestales, SERIDA, Apdo. 13, 33300, Villaviciosa, Asturias, Spain

    Elena Pérez-Vega, Ana Campa & Juan José Ferreira

  2. Department of Biología Funcional, University of Oviedo, 33006, Oviedo, Spain

    Astrid Pañeda, Cristina Rodríguez-Suárez & Ramón Giraldez

Authors
  1. Elena Pérez-Vega
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Astrid Pañeda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cristina Rodríguez-Suárez
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ana Campa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ramón Giraldez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Juan José Ferreira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ramón Giraldez.

Additional information

Communicated by H. T. Nguyen.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material (DOC 128 kb)

Supplementary material (JPG 662 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pérez-Vega, E., Pañeda, A., Rodríguez-Suárez, C. et al. Mapping of QTLs for morpho-agronomic and seed quality traits in a RIL population of common bean (Phaseolus vulgaris L.). Theor Appl Genet 120, 1367–1380 (2010). https://doi.org/10.1007/s00122-010-1261-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-010-1261-5

Keywords

Search

Navigation