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Molecular mapping of leaf rust resistance gene Lr15 in hexaploid wheat

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Abstract

Leaf rust is a widespread and commonly occurring rust disease of wheat. Genetic resistance is the most economical method of reducing losses due to leaf rust. Lr15 has been shown to be present on wheat chromosome 2D and is reported to be a seedling resistance gene. However, tightly linked markers associated with Lr15 have not been reported to date. To identify molecular markers linked to Lr15, an F2 mapping population of Thatcher × Thatcher-Lr15 was generated. Available wheat simple sequence repeat markers were utilized in parental screening and polymorphic markers were used to analyze the entire population of 221 plants. Phenotypic evaluations of the F2-derived F3 progenies with Puccinia triticina Eriks. pathotype 162A (93R15) confirmed the monogenic inheritance of Lr15. The linkage group representing chromosome 2DS was constructed at LOD 4.0 which revealed the closest flanking markers Xgwm4562 and Xgwm102 at a distance of 3.1 and 9.3 cM, respectively. Furthermore, utilization of these flanking markers in combination has successfully identified wheat lines with or without Lr15. These markers could potentially be useful in gene pyramiding with other genes to enhance rust resistance in wheat.

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Acknowledgments

Some of the SSR primers were kindly provided by the company Trait Genetics GmbH, IPK, Germany under material transfer agreement. The authors thank A. Kancharla and K.P. Kulkarni for their technical assistance and Dr. H. S. Bariana (Plant Breeding Institute, University of Sydney, Australia) for important suggestions during the study. The Director, Agharkar Research Institute, Pune is profusely thanked for field facilities. We also thank anonymous reviewers for helpful comments. Financial assistance from the Department of Biotechnology, New Delhi, India and Swiss Development Co-operation, Switzerland under Indo-Swiss Collaboration in Biotechnology is gratefully acknowledged.

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Authors and Affiliations

  1. PMB Unit, Biochemical Sciences Division, National Chemical Laboratory, Pune, 411008, India

    B. B. Dholakia, A. V. Rajwade, P. Hosmani, R. R. Khan, S. Chavan, D. M. R. Reddy, M. D. Lagu & V. S. Gupta

  2. Department of Plant Breeding, Genetics and Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India

    R. G. Saini

  3. Plant Breeding Institute-Cobbitty, Faculty of Agriculture and Environment, University of Sydney, PMB 4011, Narellan, NSW, 2567, Australia

    U. K. Bansal

Authors
  1. B. B. Dholakia
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  2. A. V. Rajwade
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  3. P. Hosmani
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  4. R. R. Khan
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  5. S. Chavan
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  6. D. M. R. Reddy
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  7. M. D. Lagu
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  8. U. K. Bansal
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  9. R. G. Saini
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  10. V. S. Gupta
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Correspondence to V. S. Gupta.

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Dholakia, B.B., Rajwade, A.V., Hosmani, P. et al. Molecular mapping of leaf rust resistance gene Lr15 in hexaploid wheat. Mol Breeding 31, 743–747 (2013). https://doi.org/10.1007/s11032-012-9813-9

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  • DOI: https://doi.org/10.1007/s11032-012-9813-9

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