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Genetic dissection of early-season cold tolerance in sorghum (Sorghum bicolor (L.) Moench)

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Abstract

Soil temperatures at 15°C or below limit germination and seedling establishment for warm season cereal crops such as sorghum (Sorghum bicolor (L.) Moench) during early-season planting. To better understand the genetics of early-season cold tolerance in sorghum, mapping of quantitative trait loci (QTL) associated with germination, emergence and vigor using a recombinant inbred mapping population was carried out. A mapping population consisting of 171 F7–F8 recombinant inbred lines (RILs) derived from the cross between RTx430 (cold-sensitive) and PI610727 (cold-tolerant) was developed and a genetic map was constructed using 141 microsatellites or simple sequence repeat (SSR) markers. The RILs were evaluated for cold and optimal temperature germinability in the laboratory, field emergence, and seedling vigor in two locations during early-season planting. Two or more QTL were detected for all traits, except for seedling vigor, with only one QTL was detected in the population. A QTL for cold germinability (Germ 12-2.1) showed the highest LOD value and was also associated with optimal germinability. One of the QTL for field emergence, Fearlygerm-9.3, a contribution from PI610727, was found significant in both locations used for the study. This study showed alignment of QTL in SBi1 (Fearlygerm-1.2 and FGerm30-1.2) with previously reported QTL associated with late field emergence identified from a different mapping population. This indicates that PI617027 shares some common loci with other known early-season cold-tolerant sorghum germplasm but also harbors novel QTL that could be useful in introgression of enhanced laboratory germination and early-season field emergence.

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References

  • Association of Official Seed Analysts (AOSA) (1999) Rules for testing seeds. AOSA, Las Cruces

    Google Scholar 

  • Bacon RK, Cantrell RP, Axtell JD (1986) Selection for seedling cold tolerance in grain sorghum. Crop Sci 26:900–903

    Article  Google Scholar 

  • Basten C, Weir B, Zeng Z (2003) QTL cartographer version 1.17. North Carolina State University, Raleigh

    Google Scholar 

  • Burow G, Franks C, Acosta-Martinez V, Xin Z (2009) Molecular mapping and characterization of profuse wax (bloom) and enhanced cuticular features of Sorghum (Sorghum bicolor (L.) Moench). Theor Appl Genet 118:423–431

    Article  PubMed  CAS  Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Cisse N, Ejeta G (2003) Genetic variation and relationships among seedling vigor traits in sorghum. Crop Sci 43:824–828

    Article  Google Scholar 

  • Doggett H (1988) Sorghum, 2nd edn. Wiley, New York

    Google Scholar 

  • Franks C, Burow G, Burke J (2006) A comparison of U.S. and Chinese sorghum germplasm for early season cold tolerance. Crop Sci 46:1371–1376

    Article  Google Scholar 

  • Fujino K, Sekiguchi H, Sato T, Kiuchi H, Nonue Y, Takeuichi Y, Ando T, Lin SY, Yano M (2004) Mapping of quantitative trait loci controlling low-temperature germinability in rice (Oryza sativa L.). Theor Appl Genet 108:794–799

    Article  PubMed  CAS  Google Scholar 

  • Fujino K, Sekiguchi H, Matsuda Y, Sugimoto K, Ono K, Yano M (2008) Molecular identification of a major quantitative trait locus, qLTG3–1, controlling low-temperature germinability in rice. PNAS 105:12623–12628

    Article  PubMed  CAS  Google Scholar 

  • Kim JS, Klein PE, Klein RR, Price HJ, Mullet JE, Stelly DM (2005) Chromosome identification and nomenclature of Sorghum bicolor. Genetics 169:1169–1173

    Article  PubMed  CAS  Google Scholar 

  • Klein RR, Rodriguez-Herrera R, Schlueter JA, Klein PE, Yu ZH, Rooney WL (2001) Identification of genomic regions that affect grain-mould incidence and other traits of agronomic importance in sorghum. Theor Appl Genet 102:307–319

    Article  CAS  Google Scholar 

  • Knoll J, Ejeta G (2008) Marker-assisted selection for early-season cold tolerance in sorghum: QTL validation across populations and environments. Theor Appl Genet 116:541–553

    Article  PubMed  Google Scholar 

  • Knoll J, Gunaratna N, Ejeta G (2008) QTL analysis of early-season cold tolerance in sorghum. Theor Appl Genet 116:577–587

    Article  PubMed  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Article  Google Scholar 

  • Lander E, Green P, Abrahamson J, Barlow A, Daly M, Stein L (1987) Mapmaker: an interactive computer package for constructing primary genetic maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Lu Q, Dahlberg J (2001) Chinese sorghum genetic resources. Econ Bot 55:401–425

    Article  Google Scholar 

  • Lu XL, Niu AL, Cai HY, Zhao Y, Liu JW, Zhu YG, Zhang ZH (2007) Genetic dissection of seedling and early vigor in a recombinant inbred line population of rice. Plant Sci 172:212–220

    Article  CAS  Google Scholar 

  • Maiti R, Raju P, Bidinger F (1981) Evaluation of visual scoring for seedling vigor in sorghum. Seed Sci Technol 9:613–622

    Google Scholar 

  • Menz MA, Klein RR, Mullet JE, Obert JA, Unruh NC, Klein PE (2002) A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP and SSR markers. Plant Mol Biol 48:483–499

    Article  PubMed  CAS  Google Scholar 

  • Miller FR (1984) Registration of RTx430 sorghum parental line. Crop Sci 24:1224

    Article  Google Scholar 

  • Rami J-F, Dufour P, Trouche G, Fliedel G, Mestres C, Davrieux F, Blanchard P, Hamon P (1998) Quantitative trait loci for grain quality, productivity, morphological and agronomical traits in sorghum (Sorghum bicolor L. Moench). Theor Appl Genet 97:605–616

    Article  CAS  Google Scholar 

  • Salas Fernandez M, Hamblin MT, Li L, Rooney WT, Tuinstra MR, Kresovich S (2008) Quantitative trait loci analysis of endosperm color and carotenoid content in sorghum grain. Crop Sci 48:1732–1743

    Article  Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTL. J Hered 93:77–78

    Article  PubMed  CAS  Google Scholar 

  • Wu YQ, Huang Y (2007) An SSR genetic map of Sorghum bicolor (L.) Moench and its comparison to a published genetic map. Genome 50:84–89

    Article  PubMed  CAS  Google Scholar 

  • Yu J, Tuinstra M (2001) Genetic analysis of seedling growth under cold temperature stress in grain sorghum. Crop Sci 41:1438–1443

    Article  Google Scholar 

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Acknowledgments

The authors would like to thank Ms. Halee Hughes and Mr. Charlie Woodfin for their technical support in this project.

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Author notes

  1. Cleve D. Franks

    Present address: Pioneer Hi-Bred Int’l. Inc., 2260 CR60 Plainview, TX, 79072, USA

Authors and Affiliations

  1. Plant Stress and Germplasm Development Unit, Cropping Systems Research Laboratory, USDA-ARS, 4th St., Lubbock, TX, 79415, USA

    Gloria Burow, John J. Burke, Zhanguo Xin & Cleve D. Franks

  2. Department of Plant and Soil Science, Texas Tech University, Lubbock, TX, 79409, USA

    Gloria Burow & John J. Burke

Authors
  1. Gloria Burow
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  2. John J. Burke
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  3. Zhanguo Xin
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  4. Cleve D. Franks
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Corresponding author

Correspondence to Gloria Burow.

Electronic supplementary material

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11032_2010_9491_MOESM1_ESM.ppt

Figure S1. Mean air and soil temperature during the early-season cold planting conducted from April 1 to April 30, 2009. (PPT 62 kb)

Figure S2. Molecular map developed in the study from the population RTx430 × PI610727. (PPT 72 kb)

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Burow, G., Burke, J.J., Xin, Z. et al. Genetic dissection of early-season cold tolerance in sorghum (Sorghum bicolor (L.) Moench). Mol Breeding 28, 391–402 (2011). https://doi.org/10.1007/s11032-010-9491-4

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  • DOI: https://doi.org/10.1007/s11032-010-9491-4

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