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Marker-assisted breeding of a photoperiod-sensitive male sterile japonica rice with high cross-compatibility with indica rice

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Abstract

The incomplete fertility of japonica × indica rice hybrids has inhibited breeders’ access to the substantial heterotic potential of these hybrids. As hybrid sterility is caused by an allelic interaction at a small number of loci, it is possible to overcome it by simple introgression at the major sterility loci. Here we report the use of marker-assisted backcrossing to transfer into the elite japonica cv. Zhendao88 a photoperiod-sensitive male sterility gene from cv. Lunhui422S (indica) and the yellow leaf gene from line Yellow249 (indica). The microsatellite markers RM276, RM455, RM141 and RM185 were used to tag the fertility genes S5, S8, S7 and S9, respectively. Line 509S is a true-breeding photoperiod-sensitive male sterile plant, which morphologically closely resembles the japonica type. Genotypic analysis showed that the genome of line 509S comprises about 92% japonica DNA. Nevertheless, hybrids between line 509S and japonica varieties suffer from a level of hybrid sterility, although the line is highly cross-compatible with indica types, with the resulting hybrids expressing a significant degree of heterosis. Together, these results suggest that segment substitution on fertility loci based on known information and marker-assisted selection are an effective approach for utilizing the heterosis of rice inter-subspecies.

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Abbreviations

CMS:

Cytoplasmic male sterility

MAS:

Marker-assisted selection

PSMS/TSMS:

Photo/temperature-sensitive male sterile

WCG:

Wide compatibility gene

WCV:

Wide-compatibility variety

YGL:

Yellow green leaf

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Acknowledgments

The current research was supported by the National High Technology Research and Development Program of China (863 program Nos. 2006AA100101 and2006AA10Z1A5), the National Science-Technology Supporting Project (Nos. 2006BAD01A01 and 2006BAD13B01), the Jiangsu Province Science-Technology Supporting Project (Nos. BE2008352 and BE2009301-3) and the 111 project (B08025).

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Authors and Affiliations

  1. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, 210095, Nanjing, China

    Liangming Chen, Zhigang Zhao, Xi Liu, Linglong Liu, Ling Jiang, Shijia Liu, Wenwei Zhang, Yihua Wang, Yuqiang Liu & Jianmin Wan

  2. Institute of Crop Science, Chinese Academy of Agricultural Sciences, 100081, Beijing, China

    Jianmin Wan

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  1. Liangming Chen
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  2. Zhigang Zhao
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  3. Xi Liu
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  4. Linglong Liu
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  5. Ling Jiang
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  7. Wenwei Zhang
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  8. Yihua Wang
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  9. Yuqiang Liu
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  10. Jianmin Wan
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Correspondence to Jianmin Wan.

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Liangming Chen and Zhigang Zhao have contributed equally to this work.

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Chen, L., Zhao, Z., Liu, X. et al. Marker-assisted breeding of a photoperiod-sensitive male sterile japonica rice with high cross-compatibility with indica rice. Mol Breeding 27, 247–258 (2011). https://doi.org/10.1007/s11032-010-9427-z

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