Skip to main content
SpringerLink
Log in

SRAP-based mapping and QTL detection for inflorescence-related traits in chrysanthemum (Dendranthema morifolium)

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Chrysanthemum (Dendranthema morifolium) is an economically important ornamental species and comprises a large proportion of the flower industry in south-east Asian and European countries. In this study, a segregating population of 142 F1 progeny of the cross between the two chrysanthemum cultivars ‘Yuhualuoying’ and ‘Aoyunhanxiao’ was used to construct two separate genetic linkage maps via a double pseudo-testcross mapping strategy. Genotyping was performed using 500 SRAP primer combinations, of which about 50% were informative. This allowed the definition of 896 SRAP loci, of which about 23% showed some segregation distortion. The ‘Yuhualuoying’ map consisted of 333 testcross markers arranged into 57 linkage groups (LGs). It covered >1,900 cM with a mean inter-marker distance of 6.9 cM. The map constructed from ‘Aoyunhanxiao’ comprised 342 test cross markers arranged into 55 LGs. It spanned nearly 1,900 cM, with a mean inter-marker distance of 6.6 cM. The markers were distributed rather uniformly along both maps. A quantitative trait loci analysis was conducted to investigate the pattern of inheritance of three inflorescence traits. This led to the detection of 12 putative loci at a LOD score >2.5, of which four each specified flower diameter, ray floret layer number, and ray floret length. This study provides molecular mapping information on marker-assisted selection programs for the improvement of multiple traits of interest.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Abe H, Nakano M, Nakatsuka A et al (2002) Genetic analysis of floral anthocyanin pigmentation traits in Asiatic hybrid lily using molecular linkage maps. Theor Appl Genet 105:1175–1182

    Article  CAS  PubMed  Google Scholar 

  • Anderson NO, Ascher PD (2000) Fertility changes in inbred families of self-incompatible chrysanthemums (Dendranthema grandiflorum). J Am Soc Hortic Sci 125(5):619–625

    Google Scholar 

  • Beedanagari SR, Dove SK, Wood BW, Conner PJ (2005) A first linkage map of pecan cultivars based on RAPD and AFLP markers. Theor Appl Genet 110:1127–1137

    Article  CAS  PubMed  Google Scholar 

  • Bennett JH (1953) Linkage in hexasomic inheritance. Heredity 7:265–283

    Article  Google Scholar 

  • Bennett JH (1954) Panmixia with tetrasomic and hexasomic inheritance. Genetics 39:150–158

    CAS  PubMed  Google Scholar 

  • Bradshaw JE, Hackett CA, Pande B et al (2008) QTL mapping of yield, agronomic and quality traits in tetraploid potato (Solanum tuberosum). Theor Appl Genet 116:193–211

    Article  PubMed  Google Scholar 

  • Brennan R, Jorgensen L, Hackett C et al (2008) The development of a genetic linkage map of blackcurrent (Ribes nigrum L.) and the identification of regions associated with key fruit quality and agronomic traits. Euphytica 161:19–34

    Article  CAS  Google Scholar 

  • Chakravarti A, Lasher L, Reefer J (1991) A maximum likelihood method for estimating genome length using genetic linkage data. Genetics 128:175–182

    CAS  PubMed  Google Scholar 

  • Chen FD, Chen PD, Li HJ (1996) Genome analysis and their phylogenetic relationships of several wild species of Dendranthema in China. Acta Hortic Sin 23(1):67–72

    Google Scholar 

  • Chen FD, Li FT, Chen SM, Guan ZY, Fang WM (2009) Meiosis and pollen germinability in small-flowered anemone type chrysanthemum cultivars. Plant Syst Evol 280:143–151

    Article  Google Scholar 

  • Crespel L, Chirollet M, Durel C, Zhang D, Meynet J, Gudin S (2002) Mapping of qualitative and quantitative phenotypic traits in Rosa using AFLP markers. Theor Appl Genet 105:1207–1214

    Article  CAS  PubMed  Google Scholar 

  • Cui NX, Chen FD, Zhao HB, Fang WM, Wang ZM (2006) Research on meiosis of some Dendranthema species and their hybrids. Acta Hortic Sin 33(5):1033–1038

    Google Scholar 

  • Dai SL, Chen JY, Li WB (1998) Application of RAPD analysis in the study on the origin of Chinese cultivated chrysanthemum. Acta Bot Sin 40(11):1053–1059

    Google Scholar 

  • Dawson CDR (1941) Tetrasomic inheritance in Lotus corniculatus L. J Genet 42:49–72

    Article  Google Scholar 

  • Dowrick GJ (1953) The chromosomes of Chrysanthemum, I: garden varieties. Heredity 7:59–72

    Article  Google Scholar 

  • Dufour P, Johnsson C, Antoine-Michard S, Cheng R, Murigneux A, Beckert M (2001) Segregation distortion at marker loci: variation during microspore embryogenesis in maize. Theor Appl Genet 102:993–1001

    Article  CAS  Google Scholar 

  • Dugo ML, Satovic Z, Millan T et al (2005) Genetic mapping of QTLs controlling horticultural traits in diploid roses. Theor Appl Genet 111:511–520

    Article  CAS  PubMed  Google Scholar 

  • Dunemann F, Kahnau R, Stange I (1999) Analysis of complex leaf and flower characters in Rhododendron using a molecular linkage map. Theor Appl Genet 98:1146–1155

    Article  CAS  Google Scholar 

  • Ferriol M, Pico B, Nuez F (2003) Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theor Appl Genet 107:271–282

    Article  CAS  PubMed  Google Scholar 

  • Gonzalo MJ, Oliver M, Garcia-Mas J et al (2005) Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theor Appl Genet 110:802–811

    Article  CAS  PubMed  Google Scholar 

  • Grattapaglia D, Sederoff R (1994) Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics 137:1121–1137

    CAS  PubMed  Google Scholar 

  • Han TH, van Eck H, de Jeu M, Jacobsen E (2002) The construction of a linkage map of Alstroemeria aurea by AFLP markers. Euphytica 128:153–164

    Article  Google Scholar 

  • Han XY, Wang LS, Liu ZA, Jan DR, Shu QY (2008) Characterization of sequence-related amplified polymorphism markers analysis of tree peony bud sports. Scientia Hortic 115:261–267

    Article  CAS  Google Scholar 

  • Hulbert S, Ilott T, Legg EJ, Lincoln S, Lander E, Michelmore R (1988) Genetic analysis of the fungus, Bremia lactucae, using restriction length polymorphism. Genetics 120:947–958

    CAS  PubMed  Google Scholar 

  • Jones A (1967) Theoretical segregation ratios of qualitatively inherited characters for hexaploid sweetpotato (Ipomoea batatas). Bull No 1368 USDA Tech

  • Kolmogoroff A (1941) Confidence limits for an unknown distribution function. Ann Math Stat 12:461–463

    Article  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Kriegner A, Cervantes JC, Burg K et al (2003) A genetic linkage map of sweet potato [Ipomoea batatas (L.) Lam.] based on AFLP markers. Mol Breed 11:169–185

    Article  CAS  Google Scholar 

  • Langton FA (1989) Inheritance in Chrysanthemum morifolium Ramat. Heredity 62:419–423

    Article  Google Scholar 

  • Lerceteau E, Guerin G, Laigret F, Denoyes-Rothan B (2003) Characterization of mixed disomic and polysomic inheritance in the octoploid strawberry (Fragaria x ananassa) using AFLP mapping. Theor Appl Genet 107:619–628

    Article  Google Scholar 

  • Li XL, Chen FD (2007) Self-incompatibility in Dendranthema morifolium. J Wuhan Bot Res 25(6):591–595

    Google Scholar 

  • Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461

    Article  CAS  Google Scholar 

  • Li C, Chen FD, Zhao HB, Fang WM (2008) Karyotype diversity of chrysanthemum cultivars with small inflorescences. Acta Hortic Sin 35(l):71–80

    CAS  Google Scholar 

  • Lilliefors HW (1967) On the Kolmogorov-Smirnov test for normality on the resolution of AFLP in plants with small genomes. Focus 18:68–69

    Google Scholar 

  • Lin ZX, Zhang YX, Zhang XL, Guo XP (2009) A high-density integrative linkage map for Gossypium hirsutum. Euphytica 166:35–45

    Article  Google Scholar 

  • Lowe KM, Walker MA (2006) Genetic linkage map of the interspecific grape rootstock cross Ramsey (Vitis champinii) × Riparia Gloire (Vitis riparia). Theor Appl Genet 112:1582–1592

    Article  CAS  PubMed  Google Scholar 

  • Maliepaard C, Jansen J, van Ooijen JW (1997) Linkage analysis in a full-sib family of an outbreeding plant species: overview and consequence for applications. Genet Res 70:235–250

    Article  Google Scholar 

  • Murray M, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

    Article  CAS  PubMed  Google Scholar 

  • Nazeer MA, Khoshoo TN (1985) Meiotic variations in Chrysanthemum morifolium complex. Nucleus (Calcutta) 28:35–41

    Google Scholar 

  • Nguyen TB, Giband M, Brottier P, Risterucci AM, Lacape JM (2004) Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers. Theor Appl Genet 109:167–175

    Article  CAS  PubMed  Google Scholar 

  • Nilsson NO, Hallden C, Hansen M, Hjerdin A, Sall T (1997) Comparing the distribution of RAPD and RFLP markers in a high density linkage map of sugar beet. Genome 40:644–651

    Article  CAS  PubMed  Google Scholar 

  • Nordenskiöld H (1953) A genetical study in the mode of segregation in hexaploid Pheum pratense. Hereditas 39:469–488

    Article  Google Scholar 

  • Oliveira K, Pinto LR, Marconi TG et al (2007) Functional integrated genetic linkage map based on EST-markers for sugarcane (Saccharum spp.) commercial cross. Mol Breed 20:189–208

    Article  CAS  Google Scholar 

  • Oyant LHS, Crespel L, Rajapakse S, Zhang L, Foucher F (2008) Genetic linkage maps of rose constructed with new microsatellite markers and locating QTL controlling flowering traits. Tree Genet Gen 4:11–23

    Google Scholar 

  • Peace C, Vithanage V, Turnbull C, Carroll B (2003) A genetic map of macadamia based on randomly amplified DNA fingerprinting (RAF) markers. Euphytica 134:17–26

    Article  CAS  Google Scholar 

  • Quillet MC, Madjidian N, Griveau Y et al (1995) Mapping genetic factors controlling pollen viability in an interspecific cross in Helianthus sect. Helianthus. Theor Appl Genet 91:1195–1202

    Article  CAS  Google Scholar 

  • Remay A, Balanne D, Thouroude T, Couviour FL, Oyant LHS, Foucher F (2009) A survey of flowering genes reveals the role of gibberellins in floral control in rose. Theor Appl Genet 119:767–781

    Article  CAS  PubMed  Google Scholar 

  • Saha MC, Mian R, Zwonitzer JC, Chekhovskiy K, Hopkins AA (2005) An SSR- and AFLP-based genetic linkage map of tall fescue (Festuca arundinacea Schred.). Theor Appl Genet 110:323–336

    Article  CAS  PubMed  Google Scholar 

  • Shen XL, Guo WZ, Lu QX et al (2007) Genetic mapping of quantitative trait loci for fiber quality and yield traits by RIL approach in upland cotton. Euphytica 155:371–380

    Article  CAS  Google Scholar 

  • Tang FP, Chen FD, Chen SM, Teng NJ, Fang WM (2009) Intergeneric hybridization and relationship of genera within the tribe Anthemideae Cass. (I. Dendranthema crassum (Kitam.) kitam. × Crossostephium chinense (L.) Makino). Euphytica 169:133–140

    Article  CAS  Google Scholar 

  • Ukoskit K, Thompson PG (1997) Autopolyploidy versus allopolyploidy and low-density randomly amplified polymorphic DNA linkage maps of sweetpotato. J Am Soc Hortic Sci 122:822–828

    CAS  Google Scholar 

  • van der Rouppe JNAM, van Zandvoort P, van Eck HJ et al (1997) Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes. Mol Gen Genet 255:438–447

    Article  Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) JoinMap v3.0, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen

    Google Scholar 

  • Wang BQ, Porter AH (2004) An AFLP-based interspecific linkage map of sympatric hybridizing colias butterflies. Genetics 168:215–225

    Article  CAS  PubMed  Google Scholar 

  • Wang CY, Chen JY, Maarten AJ (2004) Molecular evolution and phylogeny of florist’s chrysanthemum and related species. J Beijing For Univ S1:91–96

    Google Scholar 

  • Wang S, Basten JC, Zeng ZB (2007) Window QTL Cartographer Version 2.5. Statistical genetics. North Carolina State University, USA

    Google Scholar 

  • Warnke SE, Barker RE, Jung G et al (2004) Genetic linkage mapping of an annual x perennial ryegrass population. Theor Appl Genet 109:294–304

    Article  CAS  PubMed  Google Scholar 

  • Watanabe K (1977) The control of diploid-like meiosis in polyploid texa of Chrysanthemum (Compositae). Jpn J Genet 52(2):125–131

    Article  Google Scholar 

  • Watanabe K (1983) Studies on the control of diploid-like meiosis in polyploid texa of Chrysanthemum. Theor Appl Genet 66:9–14

    Article  Google Scholar 

  • Xu YF, Chen FD, Chen SM, Teng NJ, Li FT (2009) Seed set of inbred, meiosis and pollen capacity of five chrysanthemum cultivars. Acta Bot Bore-Occi Sin 29(3):469–474

    Google Scholar 

  • Yagi M, Onosaki T, Taneya M, Watanabe H, Yoshimura T, Yoshinari T, Ochiai Y, Shibata M (2006) Construction of a genetic linkage map fro the carnation by using RAPD and SSR markers and mapping quantitative trait loci (QTL) for resistance to bacterial wilt caused by Burkholderia caryophylli. J Jpn Soc Hortic Sci 75(2):166–172

    Article  CAS  Google Scholar 

  • Yasukochi Y (1998) A dense genetic linkage map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers. Genetics 150:1513–1525

    CAS  PubMed  Google Scholar 

  • Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

    CAS  PubMed  Google Scholar 

  • Zhang ZS, Hu MC, Zhang J et al (2009) Construction of a comprehensive PCR-based marker linkage map and QTL mapping for fiber quality traits in up land cotton (Gossypium hirsutum L.). Mol Breed 24:49–61

    Article  Google Scholar 

Download references

Acknowledgments

This research was financially supported by the National Nature Science Foundation of China (Grant No. 30871724).

Author information

Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China

    Fei Zhang, Sumei Chen, Fadi Chen, Weimin Fang, Yu Chen & Fengtong Li

Authors
  1. Fei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sumei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fadi Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Weimin Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fengtong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fadi Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, F., Chen, S., Chen, F. et al. SRAP-based mapping and QTL detection for inflorescence-related traits in chrysanthemum (Dendranthema morifolium). Mol Breeding 27, 11–23 (2011). https://doi.org/10.1007/s11032-010-9409-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11032-010-9409-1

Keywords

Search

Navigation