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Characterization of disease resistance gene candidates of the nucleotide binding site (NBS) type from banana and correlation of a transcriptional polymorphism with resistance to Fusarium oxysporum f.sp. cubense race 4

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Abstract

Most plant disease resistance (R) genes encode proteins with a nucleotide binding site and leucine-rich repeat structure (NBS-LRR). In this study, degenerate primers were used to amplify genomic NBS-type sequences from wild banana (Musa acuminata ssp. malaccensis) plants resistant to the fungal pathogen Fusarium oxysporum formae specialis (f. sp.) cubense (FOC) race 4. Five different classes of NBS-type sequences were identified and designated as resistance gene candidates (RGCs). The deduced amino acid sequences of the RGCs revealed the presence of motifs characteristic of the majority of known plant NBS-LRR resistance genes. Structural and phylogenetic analyses grouped the banana RGCs within the non-TIR (homology to Toll/interleukin-1 receptors) subclass of NBS sequences. Southern hybridization showed that each banana RGC is present in low copy number. The expression of the RGCs was assessed by RT-PCR in leaf and root tissues of plants resistant or susceptible to FOC race 4. RGC1, 3 and 5 showed a constitutive expression profile in both resistant and susceptible plants whereas no expression was detected for RGC4. Interestingly, RGC2 expression was found to be associated only to FOC race 4 resistant lines. This finding could assist in the identification of a FOC race 4 resistance gene.

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Acknowledgements

The authors wish to thank Mr Luke Devitt and Dr Ben Dugdale for their technical assistance, Dr Kay Taylor for providing data of NBS sequences from M. acuminata ssp. burmannicoides and Ms Jennifer Kleidon for maintaining plants. We thank Dr Ivan Buddenhagen for the provision of Musa acuminata ssp. malaccensis seed. The work was funded by the Australian Research Council. Santy Peraza-Echeverria was supported by a PhD scholarship (No. 126280) from CONACyT Mexico.

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Authors and Affiliations

  1. Centre for Tropical Crops and Biocommodities, Queensland University of Technology, GPO Box 2434, Brisbane, 4000, QLD, Australia

    Santy Peraza-Echeverria, James L. Dale, Rob M. Harding & Chris Collet

  2. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán (CICY), C. 43, No. 130, Col. Chuburna de Hidalgo, C.P. 97200, Merida, Yucatan, Mexico

    Santy Peraza-Echeverria

  3. Department of Primary Industries and Fisheries, Maroochy Research Station, Mayers Road, Nambour, 4560, QLD, Australia

    Mike K. Smith

  4. School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, 4000, QLD, Australia

    Chris Collet

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  1. Santy Peraza-Echeverria
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Correspondence to Chris Collet.

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Peraza-Echeverria, S., Dale, J.L., Harding, R.M. et al. Characterization of disease resistance gene candidates of the nucleotide binding site (NBS) type from banana and correlation of a transcriptional polymorphism with resistance to Fusarium oxysporum f.sp. cubense race 4. Mol Breeding 22, 565–579 (2008). https://doi.org/10.1007/s11032-008-9199-x

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