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Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in banana

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Abstract

The tomato Pto gene encodes a serine/threonine kinase (STK) whose molecular characterization has provided valuable insights into the disease resistance mechanism of tomato and it is considered as a promising candidate for engineering broad-spectrum pathogen resistance in this crop. In this study, a pair of degenerate primers based on conserved subdomains of plant STKs similar to the tomato Pto protein was used to amplify similar sequences in banana. A fragment of ∼550 bp was amplified, cloned and sequenced. The sequence analysis of several clones revealed 13 distinct sequences highly similar to STKs. Based on their significant similarity with the tomato Pto protein (BLASTX E value <3e-53), seven of them were classified as Pto resistance gene candidates (Pto-RGCs). Multiple sequence alignment of the banana Pto-RGC products revealed that these sequences contain several conserved subdomains present in most STKs and also several conserved residues that are crucial for Pto function. Moreover, the phylogenetic analysis showed that the banana Pto-RGCs were clustered with Pto suggesting a common evolutionary origin with this R gene. The Pto-RGCs isolated in this study represent a valuable sequence resource that could assist in the development of disease resistance in banana.

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Acknowledgements

We are grateful to Dr. Virginia Aurora Herrera Valencia and Dr. Ignacio Islas Flores for critical reading and helpful comments on the manuscript. We also thank the handling editor and two reviewers for their constructive suggestions. This work was supported financially by the Centro de Investigación Científica de Yucatán (grant no. B06) and the Consejo Nacional de Ciencia y Tecnología (grant no. 24579) from México.

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Authors and Affiliations

  1. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán (CICY), Calle 43, No. 130, Col. Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, México

    Santy Peraza-Echeverria, Andrew James-Kay, Blondy Canto-Canché & Eduardo Castillo-Castro

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  1. Santy Peraza-Echeverria
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  2. Andrew James-Kay
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  3. Blondy Canto-Canché
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  4. Eduardo Castillo-Castro
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Correspondence to Santy Peraza-Echeverria.

Additional information

Communicated by A. Hirsch.

Nucleotide sequence data are in the GenBank database as accession nos. EF492518, EF492519, EF492520, EF492521, EF492522, EF492523, EF492524, EF492525, EF492526, EF492527, EF492528, EF492529, and EF492530 for Tg-4, Tg-6, Tg-9, Tg-10, Tg-12, Tg-13, Tg-67, Tg-1, Tg-2, Tg-20, Tg-34, Tg-36, and Tg-55, respectively.

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Peraza-Echeverria, S., James-Kay, A., Canto-Canché, B. et al. Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in banana. Mol Genet Genomics 278, 443–453 (2007). https://doi.org/10.1007/s00438-007-0262-9

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  • DOI: https://doi.org/10.1007/s00438-007-0262-9

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