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Detection of QTLs Linked to Leaf and Smoke Properties in Nicotiana tabacum Based on a Study of 114 Recombinant Inbred Lines

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Abstract

Quantitative trait loci (QTLs) were investigated in a recombinant inbred line (RIL) population descended from a cross between two flue-cured Nicotiana tabacum L. inbred lines with unrelated breeding origins. A total of 59 traits, related to diverse agronomic, leaf quality, chemical composition and smoke properties were assessed. Chemical traits and smoke mutagenicity were estimated by near infrared reflectance spectroscopy (NIRS) analyses of leaf lamina powders. Physical properties of cigarettes made from each RIL, and the total particulate matter (tar and nicotine yields), benzo[a]pyrene and CO contents of the main smoke stream generated by the cigarettes in mechanical smoking tests under a standard ISO regime were also analyzed. The RILs were screened for 184 amplified fragment length polymorphism (AFLP), inter simple sequence repeat (ISSR), sequence specific amplified polymorphism (SSAP), sequence characterized amplified region (SCAR) and biological markers. A partial genetic map including 18 linkage groups was constructed based on 138 of the markers. Substantial segregation distortion (47%) was observed in linkage groups throughout the genome. Seventy-five QTLs associated with 8–41.5% of the variation in the examined traits were identified on 12 linkage groups by simple and composite interval mapping. Nineteen QTLs had opposite effects to those expected from the ranking of parental means.

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Acknowledgements

The first author is grateful for financial support from the ARN (Association pour la Recherche sur les Nicotianées). Many thanks are due to the Bergerac Tobacco Institute workers, for their excellent technical assistance in chemical analysis and field management. We thank Orléans Les Aubrais Research Center for smoking experiments and J.P. Biesse for assistance in statistical treatments. We also thank M.A. Grandbastien for helpful assistance in the SSAP experiment.

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Authors and Affiliations

  1. Altadis – Institut du Tabac, Domaine de la Tour, LBCM, 24100, Bergerac, France

    E. Julio, J.-L. Verrier & F. Dorlhac de Borne

  2. UREFV-INRA, BP 81, 33883, Villenave d’Ornon Cedex, France

    B. Denoyes-Rothan

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  1. E. Julio
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  2. B. Denoyes-Rothan
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  3. J.-L. Verrier
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  4. F. Dorlhac de Borne
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Correspondence to E. Julio.

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Julio, E., Denoyes-Rothan, B., Verrier, JL. et al. Detection of QTLs Linked to Leaf and Smoke Properties in Nicotiana tabacum Based on a Study of 114 Recombinant Inbred Lines. Mol Breeding 18, 69–91 (2006). https://doi.org/10.1007/s11032-006-9019-0

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