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Oxidative stress in rheumatoid arthritis patients: relationship to diseases activity

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Abstract

The aim of this study was to assess the oxidative stress status in rheumatoid arthritis (RA) by measuring markers of free radical production, systemic activity of disease, and levels of antioxidant. 52 RA patients and 30 healthy controls were included in the study, and clinical examination and investigations were performed and disease activity was assessed. Peripheral blood samples were used for all the assays. We assessed the markers of oxidative stress, including plasma levels of index of lipid peroxidation-thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H2O2), superoxide anion radical (O2 −), nitric oxide (NO), and superoxide dismutase activity (SOD), catalase activity (CAT) and glutathione levels in erythrocytes. In the RA group, levels of H2O2, O2 −, and TBARS were significantly higher than in controls (4.08 ± 0.31 vs. 2.39 ± 0.13 nmol/l, p < 0.01; 8.90 ± 1.28 vs. 3.04 ± 0.38 nmol/l, p < 0.01, 3.65 ± 0.55 vs. 1.06 ± 0.17 μmol/l, p < 0.01). RA patients had significantly increased SOD activity compared with healthy controls (2,918.24 ± 477.14 vs. 643.46 ± 200.63UgHbx103, p < 0.001). Patients had significantly higher levels of pro-oxidants (O2 −, H2O2, and TBARS) compared to controls, despite significantly higher levels of SOD. Significant differences were also observed in serum levels of NO in patients with high-diseases activity. Our findings support an association between oxidative/nitrosative stress and RA. Stronger response in samples with higher diseases activity suggests that oxidative/nitrosative stress markers may be useful in evaluating the progression of RA as well as in elucidating the mechanisms of disease pathogenesis.

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Acknowledgments

This work is supported by Grant no. 175043 from the Ministry of Science and Technical Development of the Republic of Serbia.

Conflict of interests

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Rheumatology, Clinical Center Kragujevac, Kragujevac, Serbia

    Mirjana Veselinovic & Aleksandra Tomic-Lucic

  2. Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

    Nevena Barudzic, Milena Vuletic, Vladimir Zivkovic & Vladimir Jakovljevic

  3. Faculty of Medicine, Institute of Physiology “Richard Burian”, Belgrade, Republic of Serbia

    Dragan Djuric

  4. Faculty of Medicine, University of Kragujevac, SvetozaraMarkovica 69, P.P. 124, 34000, Kragujevac, Republic of Serbia

    Vladimir Jakovljevic

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  1. Mirjana Veselinovic
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Correspondence to Vladimir Jakovljevic.

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Veselinovic, M., Barudzic, N., Vuletic, M. et al. Oxidative stress in rheumatoid arthritis patients: relationship to diseases activity. Mol Cell Biochem 391, 225–232 (2014). https://doi.org/10.1007/s11010-014-2006-6

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  • DOI: https://doi.org/10.1007/s11010-014-2006-6

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