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Regulation of cytokine expression in murine macrophages stimulated by excretory/secretory products from Trichinella spiralis in vitro

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Abstract

Trichinella spiralis is a zoonotic nematode and food borne parasite and infection with T. spiralis leads to suppression of the host immune response and other immunopathologies. The excretory/secretory (ES) products of T. spiralis play important roles in the process of immunomodulation. However, the mechanisms and related molecules are unknown. Macrophages, a target for immunomodulation by the helminth parasite, play a critical role in initiating and modulating the host immune response to parasite infection. In this study, we examined the effect of ES products from different stages of T. spiralis on modulating J774A.1 macrophage activities. ES products from different stages of T. spiralis reduced the capacity of macrophages to express pro-inflammatory cytokines (tumor necrosis factor α, interleukin-1β , interleukin-6 , and interleukin-12) in response to lipopolysaccharide (LPS) challenge. However, only ES products from 3-day-old adult worms and 5-day-old adult worms/new-born larvae significantly inhibited inducible nitric oxide synthase gene expression in LPS-induced macrophages. In addition, ES products alone boosted the expression of anti-inflammatory cytokines interleukin-10 and transforming growth factor-β and effector molecule arginase 1 in J774A.1 macrophages. Signal transduction studies showed that ES products significantly inhibited nuclear factor-κB translocation into the nucleus and the phosphorylation of both extracellular signal-regulated protein kinase 1/2 and p38 mitogen-activated protein kinase in LPS-stimulated J774A.1 macrophages. These results suggest that ES products regulate host immune response at the macrophage level through inhibition of pro-inflammatory cytokines production and induction of macrophage toward the alternative phenotype, which maybe important for worm survival and host health.

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Acknowledgments

This study was supported by the National S & T Major Program (Grant No. 2008ZX10004-11) and the National Natural Science Foundation of China (Grant No. 30825033, 31030064, 30972177, 30950110328, 81070311 and 31072124), most 2010CB530000 and 2011AA10A200.

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Authors and Affiliations

  1. Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, People’s Republic of China

    Xue Bai, Xiuping Wu, Xuelin Wang, Zhenhong Guan, Fei Gao, Jianli Yu, Lu Yu, Bin Tang, Xiaolei Liu, Yanxia Song, Xinrui Wang, Feng Wang & Mingyuan Liu

  2. ANSES, ENVA, UPEC, UMR BIPAR Laboratory for Animal Health, Maisons-Alfort, France

    Blaga Radu & Pascal Boireau

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  1. Xue Bai
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Correspondence to Feng Wang or Mingyuan Liu.

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Xue Bai, Xiuping Wu, Xuelin Wang and Zhenhong Guan contributed equally to this study.

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Bai, X., Wu, X., Wang, X. et al. Regulation of cytokine expression in murine macrophages stimulated by excretory/secretory products from Trichinella spiralis in vitro. Mol Cell Biochem 360, 79–88 (2012). https://doi.org/10.1007/s11010-011-1046-4

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  • DOI: https://doi.org/10.1007/s11010-011-1046-4

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