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Characterization of oligoadenylate synthetase-1 expression in rat mammary gland and prostate: effects of 17β-estradiol on the regulation of OAS1g in both tissues

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Abstract

OAS1 belongs to a protein family of interferon-induced enzymes characterized by their ability to catalyze the synthesis of 2′–5′-linked oligomers of adenosine from ATP (2-5A). 2-5A bind to the latent Ribonuclease L (RNase L), which subsequently dimerizes into the active form, acquiring the capacity of cleaving cellular and viral mRNA. Several studies indicate that OAS1 is an important inducer of apoptosis in human cancer cells and that it may be regulated by 17β-estradiol (E2). The aim of this study was to characterize OAS1 gene expression in rat mammary gland and prostate, and to analyze its regulation by E2 in both tissues. It is demonstrated that OAS1g is the most abundant OAS1 gene expressed in both tissues, and that OAS1 protein is present in the nucleus of rat mammary gland and prostate epithelial cells. In addition, it is shown by Real Time PCR that OAS1g is up-regulated by E2 in rat mammary gland, but down-regulated in prostate, suggesting that the OAS1g gene may be related to estrogen dependent pathways in rat mammary gland and prostate physiology.

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Acknowledgments

Maia CJB is recipient of a fellowship from the Portuguese Foundation for Science and Technology-FCT, SFRH/BD/13388/2003. All the experimental work was supported by the FCT plurianual program. We acknowledge Catarina Ferreira for the preparation of tissue sections and Dr Graça Baltazar for counseling in fluorescence microscopy.

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Authors and Affiliations

  1. Centre of Investigation in Health Sciences – CICS, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilha, Portugal

    C. J. B. Maia, S. Socorro & C. R. A. Santos

  2. IPATIMUP – Institute of Molecular Pathology and Immunology, University of Porto, Porto, Portugal

    F. Schmitt

  3. Medical Faculty of Porto University, Porto, Portugal

    F. Schmitt

Authors
  1. C. J. B. Maia
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  2. S. Socorro
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  3. F. Schmitt
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  4. C. R. A. Santos
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Correspondence to C. R. A. Santos.

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Maia, C.J.B., Socorro, S., Schmitt, F. et al. Characterization of oligoadenylate synthetase-1 expression in rat mammary gland and prostate: effects of 17β-estradiol on the regulation of OAS1g in both tissues. Mol Cell Biochem 314, 113–121 (2008). https://doi.org/10.1007/s11010-008-9771-z

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  • DOI: https://doi.org/10.1007/s11010-008-9771-z

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