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Twitchin as a regulator of catch contraction in molluscan smooth muscle

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Abstract

Molluscan catch muscle can maintain tension for a long time with little energy consumption. This unique phenomenon is regulated by phosphorylation and dephosphorylation of twitchin, a member of the titin/connectin family. The catch state is induced by a decrease of intracellular Ca2+ after the active contraction and is terminated by the phosphorylation of twitchin by the cAMP-dependent protein kinase (PKA). Twitchin, from the well-known catch muscle, the anterior byssus retractor muscle (ABRM) of the mollusc Mytilus, incorporates three phosphates into two major sites D1 and D2, and some minor sites. Dephosphorylation is required for re-entering the catch state. Myosin, actin and twitchin are essential players in the mechanism responsible for catch during which force is maintained while myosin cross-bridge cycling is very slow. Dephosphorylation of twitchin allows it to bind to F-actin, whereas phosphorylation decreases the affinity of the two proteins. Twitchin has been also been shown to be a thick filament-binding protein. These findings raise the possibility that twitchin regulates the myosin cross-bridge cycle and force output by interacting with both actin and myosin resulting in a structure that connects thick and thin filaments in a phosphorylation-dependent manner.

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Acknowledgements

This work was supported in part by NIH AR 042758 (to MJS and TMB), a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to DF) and a Joint Project of Japan–U.S. Cooperative Science Program of the Japan Society for the Promotion of Science (to SW).

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Authors and Affiliations

  1. Faculty of Bioresources, Mie University, Kurimamachiya 1577, 514-8507, Tsu, Mie, Japan

    Daisuke Funabara & Satoshi Kanoh

  2. Department of Physiology, Jefferson Medical College, Pennsylvania, USA

    Marion J. Siegman & Thomas M. Butler

  3. Muscle Biology Group, University of Arizona, Tucson, Arizona, USA

    David J. Hartshorne

  4. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan

    Shugo Watabe

Authors
  1. Daisuke Funabara
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  2. Satoshi Kanoh
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  3. Marion J. Siegman
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  4. Thomas M. Butler
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  5. David J. Hartshorne
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  6. Shugo Watabe
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Correspondence to Daisuke Funabara.

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Funabara, D., Kanoh, S., Siegman, M.J. et al. Twitchin as a regulator of catch contraction in molluscan smooth muscle. J Muscle Res Cell Motil 26, 455–460 (2005). https://doi.org/10.1007/s10974-005-9029-2

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