Abstract
Malaria affects over 500 million people worldwide leading to 1–2 million deaths each year, the majority of whom are children. Four Plasmodium species cause malaria in humans. To properly diagnose, and correctly treat malarial infections, accurate diagnosis of infection is required. Proper diagnosis of infection will result in a reduction of morbidity, mortality, and of drug resistant parasites. However, the current tests for malaria diagnosis do not efficiently identify the appropriate human and parasite biomarkers associated with disease. Detection of specific inflammatory mediators such as cytokines associated with malaria pathogenesis will aid the determination of disease progression, disease prognosis, and the early diagnosis of malaria infection. In this study, we used dielectric thermal analysis (DETA), thermogravimetric analysis, and differential scanning calorimetry (DSC) to characterize five human cytokines (IL-1α, IL-2, IL-4, IL-6, and IL-10), to demonstrate how their thermoanalytical properties can be investigated for sensor design. Analysis for DETA was performed at a frequency range of 0.1–300,000 Hz. Permittivity and loss factor measurements were used to calculate tan δ values. Peak frequencies were used to determine dielectric signatures for each cytokine. The peak frequencies were different for each cytokine analyzed. In addition, activation energies were frequency dependent for IL-2 but frequency independent for the remaining four cytokines. Cytokines were also examined using DSC which established variance in heat of crystallization and heat of fusion of solvent among the five cytokines. A noticeable differentiation was observed with IL-1α among the other four cytokines when analyzed using trend analysis. Detection of unique dielectric signals will aid development of sensitive dielectric sensors capable of detecting cytokines in various human samples.
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References
Clark IA, Alleva LM, Budd AC, Cowden WB. Understanding the role of inflammatory cytokines in malaria and related diseases. Travel Med Infect Dis. 2008;6:67–81.
Saleh S, Moreno-Molek S, Manthen DR, Maheswaram MPK, Sam-Yellowe T, Riga AT. Thermal behavior and signature patterns of human cytokine and soluble cytokine receptors investigated using dielectric thermal analysis and thermogravimetry. J Therm Anal Calorim. 2011. doi:10.1007/s10973-011-1530-9.
Moreno-Molek S, Saleh S, Manthen DR, Maheswaram MPK, Sam-Yellowe T, Riga AT. Predicting interactive behavior of cytokines and their receptors by dielectric thermal analysis and thermogravimetry. J Therm Anal Calorim. 2011. doi:10.1007/s10973-011-1643-1.
Presswala L, Matthews ME, Atkinson I, Najjar O, Gerhardstein N, Moran J, Wei R, Riga AT. Discovery of bound and unbound waters in crystalline amino acids revealed by thermal analysis. J Therm Anal Calorim. 2008;93:295–300.
Suherman PM, Taylor P, Smith G. Low frequency dielectric study on hydrated ovalbumin. J Non Cryst Solids. 2002;305:317–21.
Oliveberg M, Tan Y-J, Fersht AR. Negative activation enthalpies in the kinetics of protein folding. Proc Natl Acad Sci USA. 1995;92:8926–9.
Jastrzebska M, Kocot A, Vij JK, Zalewska J, Witecki T. Dielectric studies on charge hopping in melanin polymer. J Mol Struct. 2002;606:205–10.
Matthews ME, Riga AT. Effects of thermal history on solid state and melting behavior of amino acids. J Therm Anal Calorim. 2009;96:673–6.
Ruderman G, De Xammar Oro JR. On the dielectric spectra of proteins in conducting media. J Biol Phys. 1992;18:167–74.
Rizvi TZ, Khan MA. Temperature-dependent dielectric properties of slightly hydrated horn keratin. Int J Biol Macromol. 2008;42:292–7.
Kindt TJ, Goldsby RA, Osborne BA. Kuby Immunology, 6th edn. Chap 12. W.H. Freeman and Company
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Funding for this research was provided by Cleveland State University’s Undergraduate Summer Research Program 2010.
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Saleh, S., Mantheni, D.R., Maheswaram, M.P.K. et al. Human cytokines characterized by dielectric thermal analysis, thermogravimetry, and differential scanning calorimetry. J Therm Anal Calorim 111, 1707–1716 (2013). https://doi.org/10.1007/s10973-011-2077-5
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DOI: https://doi.org/10.1007/s10973-011-2077-5