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Enzyme-Linked Immunosorbent Assay (ELISA)

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Cancer Cell Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2508))

Abstract

Enzyme-linked immunosorbent assay (ELISA) is one of the most specific and straightforward assays for detecting biomolecules in research and clinics. With advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides an overall summary of the basic principle of ELISA, discusses different components of ELISA assay, and clearly outline protocols for different types of ELISA assays, including direct, indirect, sandwich, competitive, and nanoparticle-based ELISA.

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada

    Mahdis Sadat Tabatabaei & Marya Ahmed

  2. Faculty of Sustainable Design Engineering, University of Prince Edward Island, Charlottetown, PE, Canada

    Marya Ahmed

Authors
  1. Mahdis Sadat Tabatabaei
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  2. Marya Ahmed
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Corresponding author

Correspondence to Marya Ahmed .

Editor information

Editors and Affiliations

  1. Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada

    Sherri L. Christian

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Tabatabaei, M.S., Ahmed, M. (2022). Enzyme-Linked Immunosorbent Assay (ELISA). In: Christian, S.L. (eds) Cancer Cell Biology. Methods in Molecular Biology, vol 2508. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2376-3_10

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  • DOI: https://doi.org/10.1007/978-1-0716-2376-3_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2375-6

  • Online ISBN: 978-1-0716-2376-3

  • eBook Packages: Springer Protocols

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