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Preparation and characterization of poly(l,l-lactide)-b-poly(ethylene glycol)-b-poly(l,l-lactide) (PLLA-PEG-PLLA) microspheres having encapsulated tetracycline

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Abstract

Amphiphilic triblock copolymers, based on hydrophilic poly(ethylene glycol) (PEG) blocks and hydrophobic poly(l,l-lactide) (PLLA) blocks, were used as the matrix material for the preparation of tetracycline-loaded microspheres. The morphology and thermal properties of the biodegradable microspheres were evaluated. SEM showed the predominance of the spherical shape, however, it was possible to distinguish three patterns: rough or smooth surface or uneven collapsed volume. The FTIR analysis indicated good mechanical stability and structural integrity of the PLLA-PEG-PLLA copolymer’s microspheres enclosing tetracycline. By thermal analysis it was possible to see the marginal influence of tetracycline on the glass transition and melting temperatures of the PLLA-PEG-PLLA triblock copolymer, while the results by TG indicated the presence of tetracycline in the inner structure of the microspheres, which thermal decomposition leading to char formation was triggered by the drug’s presence.

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References

  1. Sudhakar Y, Kuotsu K, Bandyopadhyay AK. Buccal bioadhesive drug delivery–a promising option for orally less efficient drugs. J Control Rel. 2006;114:15–40.

    Article  CAS  Google Scholar 

  2. George M, Abraham TE. Polyionic hydrocolloids for the intestinal delivery of protein drugs: alginate and chitosan–a review. J Control Rel. 2006;114:1–15.

    Article  CAS  Google Scholar 

  3. Sajeesh S, Sharma CP. Cyclodextrin-insulin complex encapsulated polymethacrylic acid based nanoparticles for oral insulin delivery. Int J Pharm. 2006;325:17–29.

    Article  Google Scholar 

  4. Gupta KC, Ravi Kumar MNV. Drug release behavior of deads and microgranules of chitosan. Biomaterials. 2000;21:1115–30.

    Article  CAS  Google Scholar 

  5. Liu L, Li C, Li X, Yuan Z, An Y, He B. Biodegradable polylactide/poly(ethylene glycol)/polylactide triblock copolymer micelles as anticancer drug carriers. J Appl Polym Sci. 2001;80:1976–82.

    Article  CAS  Google Scholar 

  6. Deng C, Rong G, Tian H, Tang Z, Chen X, Jing X. Synthesis and characterization of poly(ethylene glycol)-b-poly(l-lactide)-b-poly(l-glutamic acid) triblock copolymer. Polymer. 2005;46:653–63.

    Article  CAS  Google Scholar 

  7. Kumar N, Ravikumar M, Domb A. Biodegradable block copolymers. Adv Drug Deliv Rev. 2001;53:23–44.

    Article  CAS  Google Scholar 

  8. Mothé CG, Drumond WS, Wang SH. Phase behavior of biodegradable amphiphilic poly(l,l-lactide)-b-poly(ethylene glycol)-b-poly(l,l-lactide). Thermochim Acta. 2006;445:61–6.

    Article  Google Scholar 

  9. Domingues ZR, Cortés ME, Gomes TA, Diniz HF, Freitas CS, Gomes JB, Faria AMC, Sinisterra RD. Bioactive glass as a drug delivery system of tetracycline and tetracycline associated with β-cyclodextrin. Biomaterials. 2004;25:327–34.

    Article  CAS  Google Scholar 

  10. Abdellaoui KS, Monti A, Barr J, Heller J, Gurny R. Optimization of a novel bioerodible device based on auto-catalyzed poly(ortho esters) for controlled delivery of tetracycline to periodontal pocket. Biomaterials. 2001;22:1–14.

    Google Scholar 

  11. Drumond WS, Mothé CG, Wang SH. Síntese e caracterização do copolímero poli (ácido lático-b-glicol etilenico). Polímeros Ciência Tecnol. 2004;14:74–9.

    CAS  Google Scholar 

  12. Bezemer JM, Radersma R, Grijpma DW, Dijkstra PJ, Van Blitterswijk CA, Feijen J. Microspheres for protein delivery prepared from amphiphilic multiblock copolymers 2. Modulation of release rate. J Control Rel. 2000;67:249–60.

    Article  CAS  Google Scholar 

  13. Yang HJ, Park IS, Na K. Biocompatible microspheres based on acetylated polysaccharide prepared from water-in-oil-water (w1/o/w2) double-emulsion method for delivery of type II diabetic drug (exenatide). Colloid Surfaces Physicochem Eng Aspect. 2009;340:115–21.

    Article  CAS  Google Scholar 

  14. Choi Y, Kim SY, Kim SH, Lee KS, Kim C, Byun Y. Long-term delivery of all-trans-retinoic acid using biodegradable PLLA/PEG-PLLA blended microspheres. Int J Pharm. 2001;215:67–81.

    Article  CAS  Google Scholar 

  15. Souza FB, Oliveira MF, Lula IS, Sansiviero MTC, Cortés ME, Sinisterra RD. Study of inclusion compound in solution involving tetracycline and β-cyclodextrin by FTIR–ATR. Vib Spectrosc. 2008;46:57–66.

    Article  Google Scholar 

  16. Drumond WS, Mothé CG, Wang SH. Quantitative analysis of biodegradable amphiphilic poly(l-lactide)block-poly(ethylene glycol)-block-poly(l-lactide) by using TG, FTIR and NMR. J Thermal Anal Calorim. 2006;85:173–7.

    Article  CAS  Google Scholar 

  17. Vila A, Sánchez A, Évora C, Soriano I, McCallion O, Alonso MJ. PLA-PEG particles as nasal protein carriers: the influence of the particle size. Int J Pharm. 2005;292:43–52.

    Article  CAS  Google Scholar 

  18. Yang YY, Chung TS, Ng NP. Morphology, drug distribution and in vitro release profiles of biodegradable polymeric microspheres containing protein fabricated by double-emulsion solvent extraction/evaporation method. Biomaterials. 2001;22:231–41.

    Article  CAS  Google Scholar 

  19. Capan Y, Jiang G, Giovagnoli S, Na KH, DeLuca PP. Preparation and characterization of poly(d,l-lactide-co-glycolide) microspheres for controlled release of human growth hormone. AAPS Pharm Sci Tech. 2003;4:1–10.

    Article  Google Scholar 

  20. Puapermpoonsiri U, Spencer J, Van der Walle CF. A freeze-dried formulation of bacteriophage encapsulated in biodegradable microspheres. Eur J Pharm Biopharm. 2009;72:26–33.

    Article  CAS  Google Scholar 

  21. Ruan G, Feng SS. Preparation and characterization of poly(lactic acid)-poly(ethylene glycol)-poly(lactic acid) (PLA-PEG-PLA) microspheres for controlled release of paclitaxel. Biomaterials. 2003;24:5037–44.

    Article  CAS  Google Scholar 

  22. Mothé CG, Azevedo AD, Drumond WS, Wang SH. Thermal properties of amphiphilic biodegradable triblock copolymer of l,l-lactide and ethylene glycol. J Thermal Anal Calorim. 2010;101(/1):229–33.

    Article  Google Scholar 

  23. Sharma RK, Chan WG, Seeman JI, Hajaligol MR. Formation of low molecular weight heterocycles and polycyclic aromatic compounds (PACs) in the pyrolysis of α-amino acids. J Anal Appl Pyrol. 2003;66:97–121.

    Article  CAS  Google Scholar 

  24. Ellis TS. Miscibility of blends of aliphatic main-chain polyesters. Macromolecules. 1995;28:1882–6.

    Article  CAS  Google Scholar 

  25. Moreno-Cerezo JM, Córdoba-Díaz M, Córdoba-Díaz D, Córdoba-Borrego M. A stability study of tetracycline and tetracycline cyclodextrins in pharmaceutical formulations. J Pharm Biom Anal. 2001;26:417–26.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Organic Process, School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil

    C. G. Mothé & A. D. Azevedo

  2. Department of Metallurgical and Materials Engineering, Polytechnic School, University of São Paulo, São Paulo, SP, 05508-900, Brazil

    W. S. Drumond & S. H. Wang

  3. Department of Chemistry, Institute of Exact Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil

    R. D. Sinisterra

Authors
  1. C. G. Mothé
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  2. A. D. Azevedo
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  3. W. S. Drumond
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  4. S. H. Wang
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  5. R. D. Sinisterra
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Correspondence to C. G. Mothé.

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Mothé, C.G., Azevedo, A.D., Drumond, W.S. et al. Preparation and characterization of poly(l,l-lactide)-b-poly(ethylene glycol)-b-poly(l,l-lactide) (PLLA-PEG-PLLA) microspheres having encapsulated tetracycline. J Therm Anal Calorim 106, 671–677 (2011). https://doi.org/10.1007/s10973-011-1655-x

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