Skip to main content
SpringerLink
Log in

Synthesis and biological distribution of 99mTc–norfloxacin complex, a novel agent for detecting sites of infection

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

The optimization of the radiolabeling yield of ciprofloxacin analogous, norfloxacin, with technetium-99m (99mTc) was described. Dependence of the labeling yield of 99mTc–norfloxacin complex on the concentration of norfloxacin, SnCl2·2H2O content, pH of the reaction mixture and reaction time was studied. Norfloxacin was labeled with 99mTc at pH 3 with a labeling yield of 95.4% by using 5 mg norfloxacin, 50 μg SnCl2·2H2O and 30 min reaction time. The formed 99mTc–norfloxacin complex was stable for a time up to 3 h. Biological distribution of 99mTc–norfloxacin complex was investigated in experimentally induced inflammation rats using Staphylococcus aureus (bacterial infection model) and heat killed Staphylococcus aureus and turpentine oil (sterile inflammation model). In case of bacterial infection, the T/NT value for 99mTc–norfloxacin complex was found to be 6.9 ± 0.4 which was higher than that of the commercially available 99mTc–ciprofloxacin under the same experimental condition.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Britton KE, Vinjamuri S, Hall AV (1997) Eur J Nucl Med 24:553–555

    CAS  Google Scholar 

  2. Rennen HJM, Boerman OC, Oyen WJG, Corstens FHM (2004) Medicinal Chem Rev 1:27–30

    Article  CAS  Google Scholar 

  3. Das SS, Hall AV, Wareham DDW, Britton KE (2002) Brazil Arch Biol Technol 45:25–28

    CAS  Google Scholar 

  4. Lupetti A, Welling MM, Paulwels EKJ, Nibbering PH (2003) Lancet Infect Dis 3:223–226

    Article  CAS  Google Scholar 

  5. Seabold JE, Palestro CJ, Brown ML (1997) J Nucl Med 38:994–997

    CAS  Google Scholar 

  6. Staab EV, Mccartney H (1978) Semin Nucl Med 8:219–223

    Article  CAS  Google Scholar 

  7. Seabold JE, Forstrom LA, Schauwecher DS (1997) J Nucl Med 38:997–999

    CAS  Google Scholar 

  8. Schrijver MD, Streule K, Senekowitsch R (1987) Nucl Med Commun 8:895–897

    Article  Google Scholar 

  9. Buscombe JR, Miller RF, Lui D (1991) Nucl Med Commun 12:583–587

    Article  CAS  Google Scholar 

  10. McAfee JG, Gagne G, Subramanian G (1991) J Nucl Med 32:2126

    CAS  Google Scholar 

  11. Akhtar MS, Qaisar A, Irfanullah J (2005) J Nucl Med 46:567–568

    CAS  Google Scholar 

  12. Akhtar MS, Iqbal J, Khan MA (2004) J Nucl Med 45:849–851

    CAS  Google Scholar 

  13. Nibbering PH, Welling MM, Paulusma-Annema A (2004) J Nucl Med 45:321–325

    CAS  Google Scholar 

  14. Gemmel MD, Nicolas MD, Welling M (2009) Semin Nucl Med 39:11–26

    Article  Google Scholar 

  15. Sankha C, Sujata SD, Susmita C, Kakali D, Mridula M, Bharat RS, Samarendu S, Shantanu G (2010) Appl Radiat Isot 68:314–316

    Article  CAS  Google Scholar 

  16. Rien HS, Huub JR, Otto CB, Rudid D, Guido S (2004) J Nucl Med 45:2088–2094

    Google Scholar 

  17. Singh AK, Verma J, Bhatnagar A, Ali AW (2003) J Nucl Med 2:103–106

    Google Scholar 

  18. Martin-Comin J, Soroa V, Rabiller G, Galli R, Cuesta L, Roca M (2004) Rev Esp Med Nucl 23:357–358

    Article  CAS  Google Scholar 

  19. Gomes Barreto V, Rabiller G, Iglesias F, Soroa V, Tubau F, Roca M (2005) Rev Esp Med Nucl 24:312–315

    Article  CAS  Google Scholar 

  20. Motaleb MA (2007) J Radioanal Nucl Chem 272:95–97

    Article  CAS  Google Scholar 

  21. Vinjamuri S, Hall AV, Solanki KK (1996) Lancet 347:233–235

    Article  CAS  Google Scholar 

  22. Hall AV, Solanki KK, Vinjamuri S, Britton KE, Sas SS (1998) J Clin Pathol 51:215–218

    Article  CAS  Google Scholar 

  23. Sonmezoglu K, Sonmezoglu M, Halac M (2001) J Nucl Med 42:567–574

    CAS  Google Scholar 

  24. Yapar Z, Kibar M, Yapar AF, Togrul E, Kayaselcuk U (2001) Eur J Nucl Med 28:822–825

    Article  CAS  Google Scholar 

  25. Larikka MJ, Ahonen AK, Niemela O (2002) Nucl Med Commun 23:167–169

    Article  CAS  Google Scholar 

  26. Dumarey N, Blocklet D, Appelboom T, Tant L, Schoutens A (2002) Eur J Nucl Med 29:530–535

    Article  CAS  Google Scholar 

  27. Welling MM, Lupetti A, Balter HS (2001) J Nucl Med 42:788–790

    CAS  Google Scholar 

  28. Sarda L, Saleh-Mghir A, Peker C, Meulemans A, Cremieux AC, Leguludec D (2002) J Nucl Med 43:239–240

    Google Scholar 

  29. Pirmettis I, Limouris GS, Papadopoulos M (1999) Eur J Nucl Med 26:1108–1111

    Google Scholar 

  30. Seung JO, Jin SR, Joong WS, Eun JY, Hyun JH (2002) Appl Radiat Isot 57:193–195

    Article  Google Scholar 

  31. Lima JET, Maliska C, Goncalves MRB, Lima RC, Corbo R (2004) World J Nucl Med 3:284–288

    Google Scholar 

  32. Sarda L, Cremieux AC, Lebellec Y, Meulemans A, Lebtahi R (2003) J Nucl Med 44:920–925

    Google Scholar 

  33. El-Ghany EA, Amine AM, El-Kawy OA, Amin M (2007) J Labelled Compd Radiopharm 50:25–29

    Article  CAS  Google Scholar 

  34. El-Ghany EA, El-Kolaly MT, Amine AM, El-Sayed AS, Abdel-Gelil F (2005) J Radioanal Nucl Chem 266:131–135

    Article  CAS  Google Scholar 

  35. Motaleb MA (2007) J Radioanal Nucl Chem 272:167–171

    Article  CAS  Google Scholar 

  36. Motaleb MA (2009) J Label Compd Radiopharm 52:415–418

    Article  CAS  Google Scholar 

  37. Yurt Lambrecht F, Durkan K, Unak P (2008) J Radioanal Nucl Chem 275:161–166

    Article  CAS  Google Scholar 

  38. Welling MM, Paulusma-Annema A, Balter HS, Pauwels EKJ, Nibbering PH (2000) Eur J Nucl Med 27:292–296

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to thank the editor and the referees of the journal for their cooperation and valuable comments and also Prof. Dr K. Farah for her efforts in reviewing the manuscript.

Author information

Authors and Affiliations

  1. Labeled Compound Department, Hot Lab Centre, Atomic Energy Authority, P.O. Box 13759, Cairo, Egypt

    I. T. Ibrahim, M. A. Motaleb & K. M. Attalah

Authors
  1. I. T. Ibrahim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Motaleb
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. M. Attalah
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. T. Ibrahim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ibrahim, I.T., Motaleb, M.A. & Attalah, K.M. Synthesis and biological distribution of 99mTc–norfloxacin complex, a novel agent for detecting sites of infection. J Radioanal Nucl Chem 285, 431–436 (2010). https://doi.org/10.1007/s10967-010-0607-4

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-010-0607-4

Keywords

Search

Navigation