Skip to main content
SpringerLink
Log in

Preparation of 99mTc-cefoperazone complex, a novel agent for detecting sites of infection

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

Prompt localization of infection sites is essential for initiating appropriate therapeutic measures. There have been major advances in the management of patients suffering from infective and/or inflammatory disorders as a result of introduction of newer drugs with high sensitivity and specificity. Since the last decade, 99mTc-ciprofloxacin was used as a biologically active radiopharmaceutical to diagnose inflammation but it has some problems related to radiochemical purity and stability. The aim of this study is to develop simple and easy formulation of cefoperazone (other broad spectrum antimicrobial agent) with 99mTc a ready to use labeling kit for infection imaging. The optimum condition that gives high labeling yield of 99mTc-cefoperazone complex, 97.9%, was achieved by using 3 mg cefoperazone, 100 μg Sn(II), at pH 8 and 10-minute reaction time. For in vivo binding of 99mTc-cefoperazone pharmacokinetic studies were carried in experimentally induced infection, in the left thigh, using Staphylococcus aureus in rats. Both thighs of the rats were dissected and counted and the ratio of bacterial infected thigh/contralateral thigh was then evaluated. The time for maximum accumulation of 99mTc-cefoperazone at the site of infection (T/NT = 4.5) was 45-minute post intravenous injection, followed by gradual decline. So, 99mTc-cefoperazone complex is simple and stable preparation for infection imaging after 45-minute post injection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. J. E. T. Lima, C. Maliska, M. R. B. Goncalves, R. C. Lima, R. Corbo, World J. Nucl. Med., 3 (2004) 284.

    Google Scholar 

  2. K. E. Britton, S. Vinjamuri, A. V. Hall et al., Eur. J. Nucl. Med., 24 (1997) 553.

    CAS  Google Scholar 

  3. J. E. Seabold, C. J. Palestro, M. L. Brown et al., J. Nucl. Med., 38 (1997) 994.

    CAS  Google Scholar 

  4. E. V. Staab, H. McCartney, Semin. Nucl. Med., 8 (1978) 219.

    CAS  Google Scholar 

  5. J. E. Seabold, L. A. Forstrom, D. S. Schauwecher et al., J. Nucl. Med., 38 (1997) 997.

    CAS  Google Scholar 

  6. M. D. Schrijver, K. Streule, R. Senekowitsch et al., Nucl. Med. Commun., 8 (1987) 895.

    Article  Google Scholar 

  7. J. R. Buscombe, R. F. Miller, D. Lui et al., Nucl. Med. Commun., 12 (1991) 583.

    Article  CAS  Google Scholar 

  8. J. G. McAfee, G. Gagne, G. Subramanian et al., J. Nucl. Med., 32 (1991) 2126.

    CAS  Google Scholar 

  9. M. S. Akhtar, A. Qaisar, J. Irfanullah et al., J. Nucl. Med., 46 (2005) 567.

    CAS  Google Scholar 

  10. M. S. Akhtar, J. Iqbal, M. A. Khan et al., J. Nucl. Med., 45 (2004) 849.

    CAS  Google Scholar 

  11. P. H. Nibbering, M. M. Welling, A. Paulusma-Annema et al., J. Nucl. Med., 45 (2004) 321.

    CAS  Google Scholar 

  12. C. J. Van Der Laken, O. C. Boerman, J. G. Oyen et al., Eur. J. Nucl. Med., 25 (1998) 535.

    Article  Google Scholar 

  13. K. E. Britton, S. Vinjamuri, A. V. Hall et al., Eur. J. Nucl. Med., 24 (1997) 553.

    CAS  Google Scholar 

  14. I. Pirmettis, G. S. Limouris, M. Papadopoulos et al., Eur. J. Nucl. Med., 26 (1999) 1108.

    Google Scholar 

  15. S. Vinjamuri, A. V. Hall, K. K. Solanki, Lancet, 347 (1996) 233.

    Article  CAS  Google Scholar 

  16. H. S. Rien, J. R. Huub, C. B. Otto, D. Rudi, S. Guido, J. Nucl. Med., 45 (2004) 2088.

    Google Scholar 

  17. J. O. seung, S. R. Jin, W. S. Joong, J. Y. Eun, J. H. Hyun et al., Appl. Radiation Isotopes, 57 (2002) 193.

    Article  Google Scholar 

  18. I. Kleisner, P. Komarek, I. Komarkova, M. Konopkova, Nuklearmedizin, 41 (2002) 224.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Labeled Compound Department, Hot Labs Center, Atomic Energy Authority, P.O. Box 13759, Cairo, Egypt

    M. A. Motaleb

Authors
  1. M. A. Motaleb
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Motaleb, M.A. Preparation of 99mTc-cefoperazone complex, a novel agent for detecting sites of infection. J Radioanal Nucl Chem 272, 167–171 (2007). https://doi.org/10.1007/s10967-006-6754-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-006-6754-y

Keywords

Search

Navigation