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Interpenetrating networks of bacterial cellulose and poly (ethylene glycol) diacrylate as potential cephalexin carriers in wound therapy

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Abstract

In this work, hybrid hydrogels based on bacterial cellulose (BC) and poly (ethylene glycol) diacrylate (PEGDA) were synthesized by a radical polymerization reaction using a redox initiator system. The proposed interpenetrating networks (IPNs) were intended for developing a controlled release micro-vesicular system for wound therapy, through micro-colloidal architectures of hydrogels based on bacterial cellulose. Therefore, the hybrid hydrogels were first characterized to determine the influence of the BC concentration on the swelling degree and their mechanical stability. Further on, infrared spectroscopy (FTIR), thermo-gravimetric analysis (TGA/DTG), and scanning electron microscopy (SEM) were implemented to investigate their structure, composition, thermal stability, and morphology. The controlled release assay of cephalexin (CEX) was performed in buffer solution at pH 7.4 and 37 °C using CEX-loaded hydrogels and the release profiles were deciphered with the aid of UV–Visible spectroscopy. Cytotoxicity tests performed on simple and CEX-loaded samples indicated that the BC-PEGDA hydrogels containing the drug of interest were relatively non-toxic when exposed to murine fibroblasts, representing thus a potential candidate for materials used for wound treatment.

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Acknowledgements

The study was funded by the Ministry of Research, Innovation and Digitization  through the Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI)[Project no. 646PED/2022 DUACTIVMER and by EU and UEFISCDI in the frame of collaborative international project 157/2020 BIOSHELL, financed under the ERA- NET CofundBlueBio2019 

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  1. Iulia Elena Neblea and Ana-Mihaela Gavrila authors with equal contribution.

Authors and Affiliations

  1. Advanced Polymer Materials and Polymer Recycling Group, The National Institute for Research&Development in Chemistry and Petrochemistry ICECHIM, Splaiul Independentei no. 202, 060021, Bucharest, Romania

    Iulia Elena Neblea, Ana-Mihaela Gavrila, Tanta‐Verona Iordache, Anamaria Zaharia, Sabina Georgiana Burlacu, Anita-Laura Chiriac & Andrei Sarbu

  2. Department of Bioresources and Polymer Science, Faculty of Applied Chemistry and Materials Science, University “Politehnica” of Bucharest, 1–7 Gh. Polizu Street, 1st District, 011061, Bucharest, Romania

    Paul Octavian Stanescu & Ionut-Cristian Radu

  3. National Institute for Chemical Pharmaceutical Research and Development ICCF, 112 Calea Vitan, 031299, Bucharest, Romania

    Georgeta Neagu

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Neblea, I.E., Gavrila, AM., Iordache, T. et al. Interpenetrating networks of bacterial cellulose and poly (ethylene glycol) diacrylate as potential cephalexin carriers in wound therapy. J Polym Res 29, 406 (2022). https://doi.org/10.1007/s10965-022-03250-9

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