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Thermodynamics, Conformation and Active Sites of the Binding of Zn–Nd Hetero-bimetallic Schiff Base to Bovine Serum Albumin

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Abstract

The interactions between N,N′-di(2-hydroxy-3-methyoxy-phenyl-1-methylene)-o-phenyldiamine-mone Zn(II), Nd(III) nitrate (2LZnNd) and bovine serum albumin (BSA) was investigated by various spectroscopic techniques under physiological conditions. It was proved that the fluorescence quenching of BSA by 2LZnNb was a result of the formation of a non-fluorescent complex with the binding constants of 3.15 × 105; 2.72 × 105 and 2.44 × 105 M–1 at 298 K, 304 K and 310 K, respectively. A marked increase in the fluorescence anisotropy in the proteinous environments indicates that BSA introduces motional restriction on the drug molecule. The corresponding thermodynamics parameters ΔH and ΔS were calculated to be –16.36 kJ mol–1 and 43.48 J mol–1 K–1 via van’t Hoff equation. Moreover, the competitive probes experiment revealed that the binding location of 2LZnNb to BSA is in the hydrophobic pocket of site II. The effect of 2LZnNb on the conformation of BSA has been analyzed by means of CD spectrum and three-dimensional fluorescence spectra. The results indicate that the conformation of BSA molecules was changed in the presence of 2LZnNb Schiff base.

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Acknowledgements

We thank Professor Rick W. K. Wong (Department of Chemistry, Hong Kong Baptist University) for providing 2LZnNd. We gratefully acknowledge financial support of Chinese 863 program (2007AA06Z407), National Natural Science Foundation of China (20873096, 30570015, 20621502), Research Foundation of Chinese Ministry of Education (20068-IRT0543), Natural Science Foundation of Hubei Province (2005ABC002).

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  1. State Key Laboratory of Virology, College of Chemistry and Molecular Science, Wuhan University, Wuhan, 430072, People’s Republic of China

    Qi Xiao, Yi Liu, Fang-fang Tian & Jun-cheng Zhu

  2. Research Center of Analytical Science, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Shan Huang

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  1. Qi Xiao
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Correspondence to Yi Liu.

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Xiao, Q., Huang, S., Liu, Y. et al. Thermodynamics, Conformation and Active Sites of the Binding of Zn–Nd Hetero-bimetallic Schiff Base to Bovine Serum Albumin. J Fluoresc 19, 317–326 (2009). https://doi.org/10.1007/s10895-008-0418-y

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