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A New Cold-Adapted, Organic Solvent Stable Lipase from Mesophilic Staphylococcus epidermidis AT2

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Abstract

The gene encoding a cold-adapted, organic solvent stable lipase from a local soil-isolate, mesophilic Staphylococcus epidermidis AT2 was expressed in a prokaryotic system. A two-step purification of AT2 lipase was achieved using butyl sepharose and DEAE sepharose column chromatography. The final recovery and purification fold were 47.09 % and 3.45, respectively. The molecular mass of the purified lipase was estimated to be 43 kDa. AT2 lipase was found to be optimally active at pH 8 and stable at pH 6–9. Interestingly, this enzyme demonstrated remarkable stability at cold temperature (<30 °C) and exhibited optimal activity at a temperature of 25 °C. A significant enhancement of the lipolytic activity was observed in the presence of Ca2+, Tween 60 and Tween 80. Phenylmethylsulfonylfluoride, a well known serine inhibitor did not cause complete inhibition of the enzymatic activity. AT2 lipase exhibited excellent preferences towards long chain triglycerides and natural oils. The lipolytic activity was stimulated by dimethylsulfoxide and diethyl ether, while more than 50 % of its activity was retained in methanol, ethanol, acetone, toluene, and n-hexane. Taken together, AT2 lipase revealed highly attractive biochemical properties especially because of its stability at low temperature and in organic solvents.

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Abbreviations

A600 :

Absorbance at 600 nm wavelength

ßME:

2-mercaptoethanol

EDTA:

Ethylenediamineteraacetic acid

DMSO:

Dimethylsulfoxide

DTT:

Dithiothreitol

IPTG:

Isopropyl β-d-1thiogalactopyranoside

OD:

Optical density

PCR:

Polymerase chain reaction

PMSF:

Phenylmethylsulfonylfluoride

PVDF:

Polyvinylidene fluoride

SDS:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

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Acknowledgments

We thank Prof Dr. Andrew H.-J Wang and Dr. Lee Cheng-Chung from Institute of Biological Chemistry, Academia Sinica, Taiwan for AUC analysis. This project was supported by Ministry of Science and Technology, Malaysia [02-01-04 SF 1024].

Conflict of interest

The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Enzyme and Microbial Technology Research Centre, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Nor Hafizah Ahmad Kamarudin, Raja Noor Zaliha Raja Abd. Rahman, Mohd Shukuri Mohamad Ali, Thean Chor Leow, Mahiran Basri & Abu Bakar Salleh

  2. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Nor Hafizah Ahmad Kamarudin, Raja Noor Zaliha Raja Abd. Rahman, Mohd Shukuri Mohamad Ali, Thean Chor Leow & Abu Bakar Salleh

  3. Faculty of Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Mahiran Basri

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  1. Nor Hafizah Ahmad Kamarudin
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  2. Raja Noor Zaliha Raja Abd. Rahman
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Correspondence to Raja Noor Zaliha Raja Abd. Rahman.

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Kamarudin, N.H.A., Rahman, R.N.Z.R.A., Ali, M.S.M. et al. A New Cold-Adapted, Organic Solvent Stable Lipase from Mesophilic Staphylococcus epidermidis AT2. Protein J 33, 296–307 (2014). https://doi.org/10.1007/s10930-014-9560-3

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