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Fluorescence Study of Lipid Bilayer Interactions of Eu(III) Coordination Complexes

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Abstract

The interaction between Eu(III) tris-β-diketonato coordination complexes (EC), displaying antitumor activity, and lipid vesicles composed of zwitterionic lipid phosphatidylcholine has been studied using fluorescence spectroscopy techniques. To characterize EC-membrane binding, several fluorescent probes, including pyrene, Prodan and 1,6-diphenyl-1,3,5-hexatriene, have been employed. It has been found that EC display effective partitioning into lipid phase, giving rise to structural modifications of both polar and nonpolar lipid bilayer regions, viz. enhancement of membrane hydration and increase in tightness of lipid chain packing. The fact that EC accumulating in lipid bilayer are incapable of inducing significant disruption of membrane structural integrity creates strong prerequisites for development of liposomal nanocarriers of these potential antitumor drugs. Such a possibility is also corroborated by the observation that EC membrane incorporation does not prevent lipid bilayer partitioning of long-wavelength squaraine dyes which represent promising candidates for visualization of liposome biodistribution.

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Authors and Affiliations

  1. Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov, 61077, Ukraine

    Olga K. Kutsenko, Valeriya M. Trusova & Galyna P. Gorbenko

  2. Department of Applied Organic Chemistry, Faculty of Chemistry, University of Sofia, 1164, Sofia, Bulgaria

    Todor Deligeorgiev, Aleksey Vasilev, Stefka Kaloianova & Nedyalko Lesev

  3. 19–32 Geroyev Truda St., Kharkov, 61144, Ukraine

    Valeriya M. Trusova

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  1. Olga K. Kutsenko
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  2. Valeriya M. Trusova
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  3. Galyna P. Gorbenko
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  4. Todor Deligeorgiev
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Correspondence to Valeriya M. Trusova.

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Kutsenko, O.K., Trusova, V.M., Gorbenko, G.P. et al. Fluorescence Study of Lipid Bilayer Interactions of Eu(III) Coordination Complexes. J Fluoresc 21, 1689–1695 (2011). https://doi.org/10.1007/s10895-011-0861-z

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  • DOI: https://doi.org/10.1007/s10895-011-0861-z

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