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Rhod-5N as a Fluorescent Molecular Sensor of Cadmium(II) Ion

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Abstract

The photophysical and complexing properties of Rhod-5N (commercially available) in MOPS buffer are reported. This fluorescent molecular sensor consists of a BAPTA chelating moiety bound to a rhodamine fluorophore. Its fluorescence quantum yield is low and a drastic enhancement of fluorescence intensity upon cation binding was observed. Special attention was paid to the complexation with Cd2+, a well known toxic metal ion. Possible interference with other metal ions (Na+, K+, Mg2+, Ca2+, Zn2+, Pb2+) was examined. Rhod-5N was found to be highly selective of Cd2+ over those interfering cations except Pb2+. The limit of detection is 3.1 μg l−1.

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Authors and Affiliations

  1. Laboratoire de Chimie Générale, Conservatoire National des Arts et Métiers, 292 rue Saint-Martin, F-75141, Paris Cedex 03, France

    Mathieu Soibinet, Vincent Souchon, Isabelle Leray & Bernard Valeur

  2. Laboratoire de Photophysique et Photochimie Supramoléculaires et Macromoléculaires, École Normale Supérieure de Cachan, 61 Avenue du Président Wilson, F-94235, Cachan Cedex, France

    Mathieu Soibinet, Vincent Souchon, Isabelle Leray & Bernard Valeur

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  1. Mathieu Soibinet
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  2. Vincent Souchon
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  3. Isabelle Leray
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  4. Bernard Valeur
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Correspondence to Bernard Valeur.

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Soibinet, M., Souchon, V., Leray, I. et al. Rhod-5N as a Fluorescent Molecular Sensor of Cadmium(II) Ion. J Fluoresc 18, 1077–1082 (2008). https://doi.org/10.1007/s10895-008-0352-z

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  • DOI: https://doi.org/10.1007/s10895-008-0352-z

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