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On Mechanism of Intermediate-Sized Circular DNA Compaction Mediated by Spermine: Contribution of Fluorescence Lifetime Correlation Spectroscopy

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Abstract

The compaction of DNA plays a role in the nuclei of several types of cells and becomes important in the non-viral gene therapy. Thus, it is in the scope of research interest. It was shown, that spermine-induced compaction of large DNA molecules occurs in a discrete “all-or-non” regime, where the coexistence of free and folded DNA molecules was observed. In the case of intermediate-sized DNA molecules (~10 kbp), so far, it was stated that the mechanism of folding is continuous. Here, we show, that neither a standard benchmark technique—dynamic light scattering, nor a single molecule technique such as fluorescence correlation spectroscopy, can decide what kind of mechanism is undertaken in the compaction process. Besides, we introduce an application of a new approach—fluorescence lifetime correlation spectroscopy. The method takes an advantage of a subtle lifetime change of an intercalating dye PicoGreen® during the titration with spermine and based on that, it reveals the discrete mechanism of the process. Furthermore, we show that it allows for observation of the equilibrium state transition dynamics.

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Acknowledgement

We acknowledge support of the Ministry of Education of the Czech Republic via grant LC06063 (JH, AB, MH) and long-term research project MSM0021620857 (MŠ, KP) and the Academy of Sciences of the Czech Republic via grant IAA400400621 (TK, MŠ).

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Authors and Affiliations

  1. J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejškova 3, 182 23, Prague 8, Czech Republic

    Jana Humpolíčková, Teresa Kral, Aleš Benda & Martin Hof

  2. Department of Physical and Macromolecular Chemistry and Laboratory of Specialty Polymers, School of Science, Charles University in Prague, Albertov 2030, 128 40, Prague 2, Czech Republic

    Miroslav Štěpánek & Karel Procházka

  3. Department of Physics and Biophysics, Agricultural University, Norwida 25, 50-375, Wrocław, Poland

    Teresa Kral

Authors
  1. Jana Humpolíčková
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  2. Miroslav Štěpánek
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  3. Teresa Kral
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  4. Aleš Benda
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  5. Karel Procházka
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  6. Martin Hof
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Correspondence to Martin Hof.

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Humpolíčková, J., Štěpánek, M., Kral, T. et al. On Mechanism of Intermediate-Sized Circular DNA Compaction Mediated by Spermine: Contribution of Fluorescence Lifetime Correlation Spectroscopy. J Fluoresc 18, 679–684 (2008). https://doi.org/10.1007/s10895-008-0345-y

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  • DOI: https://doi.org/10.1007/s10895-008-0345-y

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