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Green Synthesis and Characterization of Silver Nanoparticles Using Ascomycota Fungi Penicillium nalgiovense AJ12

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Abstract

A new method for green synthesis of silver nanoparticles using the cell-free filtrate of Penicillium nalgiovense AJ12 as reducing and protecting agent was described. The pathway is based on the reduction of Ag1+ by protein(s). Various techniques such as UV–Vis spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared and Zeta potential measurements were used to characterize the silver nanoparticle obtained. The results revealed synthesis of the spherical silver nanoparticles coated with protein(s). The average size of the particles obtained from TEM was 15.2 ± 2.6 nm. DLS measurements showed that the particle size was higher than that estimated from TEM measurements and was 25.2 ± 2.8 nm. Studies on the role of the cell-free filtrate proteins in the synthesis of silver nanoparticles indicated that the process is non-enzymatic but involves amino acids interactions with silver ions. It was found that the aqueous silver nanoparticles suspensions exhibited excellent stability over a wide range of ionic strength, pH and temperature.

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References

  1. V. A. Nadtochenko, M. A. Radtsig, and I. A. Khmel (2010). Nonotechnol. Russia 5, 277–289.

    Article  Google Scholar 

  2. K. B. Narayanan and N. Sakthivel (2010). Adv. Colloid Interface Sci. 156, 1–13.

    Article  CAS  Google Scholar 

  3. A. Jebali, F. Ramezani, and B. Kazemi (2011). J. Clust. Sci. 22, 225–232.

    Article  CAS  Google Scholar 

  4. X. Zhang, S. Yan, R. D. Tyagi, and R. Y. Surampalli (2011). Chemosphere 82, 489–494.

    Article  CAS  Google Scholar 

  5. N. Durán, P. D. Marcato, M. Durán, A. Yadav, A. Gade, and M. Rai (2011). Appl. Microbiol. Biotechnol. 90, 1609–1624.

    Article  Google Scholar 

  6. I. Maliszewska Microbial synthesis of metal nanoparticles. in M. Rai and N. Duran (eds.), Metal Nanoparticles in Microbiology (Springer, Berlin, 2011), pp. 153–176.

    Chapter  Google Scholar 

  7. A. Ahmad, P. Mukherjee, P. Senapati, D. Mandal, M. I. Khan, R. Kumar, and M. Sastry (2003). Colloids Surf. B 28, 313–318.

    Article  CAS  Google Scholar 

  8. N. Durán, P. D. Marcato, O. L. Alves, G. I. H. De Souza, and E. Esposito (2005). J. Nanobiotechnol. 3, 8. doi:10.1186/1477-3155-3-8.

    Article  Google Scholar 

  9. A. Ingle, A. Gade, S. Pierrat, C. Sonnichsen, and M. K. Rai (2008). Curr. Nanosci. 4, 141–144.

    Article  CAS  Google Scholar 

  10. S. A. Kumar, M. K. Abyaneh, S. W. Gosavi, S. K. Kulkarni, R. Pasricha, A. Ahmad, and M. I. Khan (2007). Biotechnol. Lett. 29, 439–443.

    Article  CAS  Google Scholar 

  11. H. Moteshafi, S. M. Mousavi, and S. A. Shojaosadati (2012). J. Ind. Eng. Chem. 18, 2046–2050.

    Article  CAS  Google Scholar 

  12. R. Sanghi and P. Verma (2009). Bioresour Technol. 100, 501–504.

    Article  CAS  Google Scholar 

  13. P. Mukherjee, M. Roy, B. P. Mandal, G. K. Dey, P. K. Mukherjee, J. Ghatak, A. K. Tyagi, and S. P. Kale (2008). Nanotechnology 19, 1–7.

    Google Scholar 

  14. A. R. Binupriya, M. Sathishkumar, K. Vijayaraghavan, and S. I. Yun (2010). J. Hazard. Mater. 177, 539–545.

    Article  CAS  Google Scholar 

  15. A. R. Binupriya, M. Sathishkumar, and S. I. Yun (2010). Colloids. Surf. B 79, 531–534.

    Article  CAS  Google Scholar 

  16. J. I. Pitt The Genus Penicillium and its Teleomorphic States Eupenicillium and Talaromyces (Academic Press, New York, 1979).

    Google Scholar 

  17. K. B. Raper and C. Thom A Manual of the Penicillia (Williams & Wilkins, Baltimore, 1949).

    Google Scholar 

  18. O. Filtenborg, J. C. Frisvad, and U. Trane The Significance of Yeast Extract Composition of Metabolite Production in Penicillium. in R. A. Samson and J. I. Pitt (eds.), Modern Concepts in Penicillium and Aspergillus Classification (Plenum Press, New York, 1990).

    Google Scholar 

  19. J. C. Frisvad (1993). Lett. Appl. Microbiol. 16, 154–157.

    Article  CAS  Google Scholar 

  20. R. W. Riddle (1950). Mycologia 42, 265–270.

    Article  Google Scholar 

  21. M. M. Bradford (1976). Anal. Biochem. 72, 248–254.

    Article  CAS  Google Scholar 

  22. R. J. Simpson Purifying Proteins for Proteomics: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 2004).

    Google Scholar 

  23. U. K. Laemmli (1970). Nature 227, 680–685.

    Article  CAS  Google Scholar 

  24. J. H. Morissey (1981). Anal Chem. 117, 307–310.

    Google Scholar 

  25. S. Patil, J. Fernandes, R. Tangasali, and I. Furtado (2013). J. Clust. Sci. doi:10.1007/s10876-013-0621-0.

  26. M. Singh, M. Kumar, R. Kalaivani, S. Manikandan, and A. K. Kumaraguru (2013). Bioprocess Biosyst. Eng. 36, 407–415.

    Article  CAS  Google Scholar 

  27. R. Sanghi, P. Verma, and S. Puri (2011). Adv. Chem. Eng. Sci. 1, 154–162.

    Article  CAS  Google Scholar 

  28. A. Gole, C. V. Dash, V. Ramachandran, A. B. Mandale, R. Sainkar, M. Rao, and M. Sastry (2001). Langmuir 17, 1674.

    Article  CAS  Google Scholar 

  29. P. Daizy, C. Unni, S. A. Aromal, and V. K. Vidhu (2011). Spectrochim Acta Part A 78, 899–904.

    Article  Google Scholar 

  30. A. Rangnekar, T. K. Sarma, A. K. Singh, J. Dek, A. Ramesh, and A. Chattopadhyay (2007). Langmuir 23, 5700–5706.

    Article  CAS  Google Scholar 

  31. J. Xie, J. Y. Lee, D. I. C. Wang, and Y. P. Ting (2007). ACS Nano 1, 429–439.

    Article  CAS  Google Scholar 

  32. N. Jain, A. Bhargava, J. C. Tarafdar, S. K. Singh, and J. Panwar (2013). Appl. Microbiol. Biotechnol. 97, 859–869.

    Article  CAS  Google Scholar 

  33. M. Aslam, L. Fu, M. Su, K. Vijayamohanan, and V. P. Dravid (2004). J. Mater. Chem. 14, 1795–1797.

    Article  CAS  Google Scholar 

  34. P. Sharma, S. Sathyanarayana, P. Kumar, and K. C. Gupta (1990). Anal. Biochem. 189, 173–177.

    Article  CAS  Google Scholar 

  35. M. Roy, P. Mukherjee, B. P. Mandal, R. K. Sharma, A. K. Tyagi, and S. P. Kale (2012). RSC Adv. 2, 6496–6503.

    Article  CAS  Google Scholar 

  36. I. Ojea-JiméneI and V. Puntes (2009). J. Am. Chem. Soc. 131, 13320–13327.

    Article  Google Scholar 

  37. S. Si and T. K. Mandal (2007). Langmuir 23, 190–195.

    Article  CAS  Google Scholar 

  38. G. Wang and W. Sun (2006). J. Phys. Chem. B 110, 20901–20905.

    Article  CAS  Google Scholar 

  39. S. K. Das, C. Dickinson, F. Lafir, D. F. Brougham, and E. Marsili (2012). Green Chem. 14, 1322–1334.

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by NCN (Grant No. NN 507 5150 58) and by a statutory activity subsidy from the Polish Ministry of Science and Higher Education for the Faculty of Chemistry of Wrocław University of Technology.

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Authors and Affiliations

  1. Division of Medicinal Chemistry and Microbiology, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland

    Irena Maliszewska & Anna Juraszek

  2. Department of Biochemistry, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland

    Katarzyna Bielska

Authors
  1. Irena Maliszewska
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  2. Anna Juraszek
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  3. Katarzyna Bielska
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Correspondence to Irena Maliszewska.

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Maliszewska, I., Juraszek, A. & Bielska, K. Green Synthesis and Characterization of Silver Nanoparticles Using Ascomycota Fungi Penicillium nalgiovense AJ12. J Clust Sci 25, 989–1004 (2014). https://doi.org/10.1007/s10876-013-0683-z

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  • DOI: https://doi.org/10.1007/s10876-013-0683-z

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