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On the properties of calcium-induced permeability transition in neonatal heart mitochondria

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Abstract

Permeability transition was examined in heart mitochondria isolated from neonate rats. We found that these mitochondria were more susceptible to Ca2+-induced membrane leakiness than mitochondria from adult rats. In K+ containing medium, at 25 °C, mitochondria were unable to accumulate Ca2+. Conversely, in Na+ containing medium, mitochondria accumulated effectively Ca2+. At 15 °C mitochondria accumulated Ca2+ regardless of the presence of K+. Kinetics of Ca2+ accumulation showed a similar Vmax as that of adult mitochondria. Lipid milieu of inner membrane contained more unsaturated fatty acids than adult mitochondria. Aconitase inhibition and high thiobarbituric acid-reactive substances (TBARS) indicate that oxidative stress caused mitochondrial damage. In addition, proteomics analysis showed that there is a considerable diminution of succinate dehydrogenase C and subunit 4 of cytochrome oxidase in neonate mitochondria. Our proposal is that dysfunction of the respiratory chain makes neonate mitochondria more susceptible to damage by oxidative stress.

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Authors and Affiliations

  1. Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, Juan Badiano # 1, Tlalpam, Mexico, DF, 014080, Mexico

    Natalia Pavón, Juan Carlos Gallardo, Luz María Hernández-Esquivel, Mabel Buelna-Chontal, Cecilia Zazueta, Sara Rodríguez-Enríquez & Edmundo Chávez

  2. Departamento de Biomedicina, Cardiovascular, Instituto Nacional de Cardiología, Ignacio Chávez, Mexico, DF, 014080, Mexico

    Mohammed El-Hafidi

Authors
  1. Natalia Pavón
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  2. Juan Carlos Gallardo
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  3. Luz María Hernández-Esquivel
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  4. Mohammed El-Hafidi
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  5. Mabel Buelna-Chontal
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  6. Cecilia Zazueta
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  7. Sara Rodríguez-Enríquez
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  8. Edmundo Chávez
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Correspondence to Edmundo Chávez.

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Pavón, N., Gallardo, J.C., Hernández-Esquivel, L.M. et al. On the properties of calcium-induced permeability transition in neonatal heart mitochondria. J Bioenerg Biomembr 43, 757–764 (2011). https://doi.org/10.1007/s10863-011-9401-4

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  • DOI: https://doi.org/10.1007/s10863-011-9401-4

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