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An Inception Report on the TOM Complex of the Amoeba Acanthamoeba castellanii, a Simple Model Protozoan in Mitochondria Studies

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Abstract

It is suggested that in the course of the TOM complex evolution at least two lineages have appeared: the animal–fungal and green plant ones. The latter involves also the TOM complexes of algae and protozoans. The amoeba Acanthamoeba castellanii is a free-living nonphotosynthetic soil protozoan, whose mitochondria share many bioenergetic properties with mitochondria of plants, animals and fungi. Here, we report that a protein complex, identified electrophysiologically as the A. castellanii TOM complex, contains a homologue of yeast/animal Tom70. Further, molecular weight of the complex (about 500 kDa) also points to A. castellanii evolutionary relation with fungi and animal. Thus, the data indicates that the TOM complex of A. castellanii is not a typical example of the protozoan TOM complex.

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Abbreviations

DDM:

n-dodecyl β-d-maltoside

DHFR:

dihydrofolate reductase

ES-MS/MS:

electrospray ionization tandem mass spectrometry

MALDI-MS:

matrix-assisted laser desorption/ionization mass spectrometry

MOPS:

4-morpholinopropanesulfonic acid

TOM:

translocase of the outer membrane

VDAC:

voltage-dependent anion-selective channel

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Authors and Affiliations

  1. Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland

    Malgorzata Wojtkowska, Natalia Szczech, Olgierd Stobienia, Wieslawa Jarmuszkiewicz, Małgorzata Budzinska & Hanna Kmita

  2. Laboratory of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland

    Hanna Kmita

Authors
  1. Malgorzata Wojtkowska
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  2. Natalia Szczech
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  3. Olgierd Stobienia
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  4. Wieslawa Jarmuszkiewicz
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  5. Małgorzata Budzinska
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  6. Hanna Kmita
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Correspondence to Hanna Kmita.

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Wojtkowska, M., Szczech, N., Stobienia, O. et al. An Inception Report on the TOM Complex of the Amoeba Acanthamoeba castellanii, a Simple Model Protozoan in Mitochondria Studies. J Bioenerg Biomembr 37, 261–268 (2005). https://doi.org/10.1007/s10863-005-6636-y

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  • DOI: https://doi.org/10.1007/s10863-005-6636-y

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