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Dual effects of β-cyclodextrin-stabilised silver nanoparticles: enhanced biofilm inhibition and reduced cytotoxicity

  • Engineering and Nano-engineering Approaches for Medical Devices
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Abstract

The composition and mode of synthesis of nanoparticles (NPs) can affect interaction with bacterial and human cells differently. The present work describes the ability of β-cyclodextrin (β-CD) capped silver nanoparticles (AgNPs) to inhibit biofilm growth and reduce cytotoxicity. Biofilm formation of Staphylococcus epidermidis CSF 41498 was quantified by a crystal violet assay in the presence of native and capped AgNPs (Ag-10CD and Ag-20CD), and the morphology of the biofilm was observed by scanning electron microscope. The cytotoxicity of the AgNPs against HaCat cells was determined by measuring the increase in intracellular reactive oxygen species and change in mitochondrial membrane potential (ΔΨm). Results indicated that capping AgNPs with β-CD improved their efficacy against S. epidermidis CSF 41498, reduced biofilm formation and their cytotoxicity. The study concluded that β-CD is an effective capping and stabilising agent that reduces toxicity of AgNPs against the mammalian cell while enhancing their antibiofilm activity.

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References

  1. Abbasi E, Milani M, Fekri Aval S, Kouhi M, Akbarzadeh A, Tayefi Nasrabadi H, Nikasa P, Joo SW, Hanifehpour Y, Nejati-Koshki K. Silver nanoparticles: synthesis methods, bio-applications and properties. Crit Rev Microbiol. 2004;0:1–8.

    Google Scholar 

  2. Maynard AD, Kuempel ED. Airborne nanostructured particles and occupational health. J Nanopart Res. 2005;7(6):587–614.

    Article  Google Scholar 

  3. AshaRani P, Low Kah Mun G, Hande MP, Valiyaveettil S. Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano. 2008;3(2):279–90.

    Article  Google Scholar 

  4. Choi O, Yu C-P, Esteban Fernández G, Hu Z. Interactions of nanosilver with Escherichia coli cells in planktonic and biofilm cultures. Water Res. 2010;44(20):6095–103.

    Article  Google Scholar 

  5. Rhim J-W, Park H-M, Ha C-S. Bio-nanocomposites for food packaging applications. Prog Polym Sci. 2013;38(10):1629–52.

    Article  Google Scholar 

  6. Hebeish A, El-Naggar M, Fouda MM, Ramadan M, Al-Deyab SS, El-Rafie M. Highly effective antibacterial textiles containing green synthesized silver nanoparticles. Carbohydr Polym. 2011;86(2):936–40.

    Article  Google Scholar 

  7. Maillard J-Y, Hartemann P. Silver as an antimicrobial: facts and gaps in knowledge. Crit Rev Microbiol. 2013;39(4):373–83.

    Article  Google Scholar 

  8. Stobie N, Duffy B, McCormack DE, Colreavy J, Hidalgo M, McHale P, Hinder SJ. Prevention of Staphylococcus epidermidis biofilm formation using a low-temperature processed silver-doped phenyltriethoxysilane sol-gel coating. Biomaterials. 2008;29(8):963–9.

    Article  Google Scholar 

  9. Chairuangkitti P, Lawanprasert S, Roytrakul S, Aueviriyavit S, Phummiratch D, Kulthong K, Chanvorachote P, Maniratanachote R. Silver nanoparticles induce toxicity in A549 cells via ROS-dependent and ROS-independent pathways. Toxicol In Vitro. 2013;27(1):330–8.

    Article  Google Scholar 

  10. Grosse S, Evje L, Syversen T. Silver nanoparticle-induced cytotoxicity in rat brain endothelial cell culture. Toxicol In Vitro. 2013;27(1):305–13.

    Article  Google Scholar 

  11. Mohanty S, Mishra S, Jena P, Jacob B, Sarkar B, Sonawane A. An investigation on the antibacterial, cytotoxic, and antibiofilm efficacy of starch-stabilized silver nanoparticles. Nanomed: Nanotech Biol Med. 2012;8(6):916–24.

    Google Scholar 

  12. Abdelgawad AM, Hudson SM, Rojas OJ. Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol) systems. Carbohydr Polym. 2014;100:166–78.

    Article  Google Scholar 

  13. Andrade PF, de Faria AF, da Silva DS, Bonacin JA, Gonçalves MDC. Structural and morphological investigations of β-cyclodextrin-coated silver nanoparticles. Colloids Surf B Biointerfaces. 2014;118:289–97.

    Article  Google Scholar 

  14. Singh S, Patel P, Jaiswal S, Prabhune A, Ramana C, Prasad B. A direct method for the preparation of glycolipid–metal nanoparticle conjugates: sophorolipids as reducing and capping agents for the synthesis of water re-dispersible silver nanoparticles and their antibacterial activity. New J Chem. 2009;33(3):646–52.

    Article  Google Scholar 

  15. Suresh AK, Pelletier DA, Wang W, Morrell-Falvey JL, Gu B, Doktycz MJ. Cytotoxicity induced by engineered silver nanocrystallites is dependent on surface coatings and cell types. Langmuir. 2012;28(5):2727–35.

    Article  Google Scholar 

  16. Loftsson T, Brewster ME. Cyclodextrins as functional excipients: methods to enhance complexation efficiency. J Pharm Sci. 2012;101(9):3019–32.

    Article  Google Scholar 

  17. Bazaka K, Jacob MV, Crawford RJ, Ivanova EP. Efficient surface modification of biomaterial to prevent biofilm formation and the attachment of microorganisms. Appl Microbiol Biotechnol. 2012;95(2):299–311.

    Article  Google Scholar 

  18. Jaiswal S, Duffy B, Jaiswal AK, Stobie N, McHale P. Enhancement of the antibacterial properties of silver nanoparticles using β-cyclodextrin as a capping agent. Int J Antimicrob Ag. 2010;36(3):280–3.

    Article  Google Scholar 

  19. Erriu M, Genta G, Tuveri E, Orrù G, Barbato G, Levi R. Microtiter spectrophotometric biofilm production assay analyzed with metrological methods and uncertainty evaluation. Measurement. 2012;45(5):1083–8.

    Article  Google Scholar 

  20. Mukherjee SG, O’Claonadh N, Casey A, Chambers G. Comparative in vitro cytotoxicity study of silver nanoparticle on two mammalian cell lines. Toxicol In Vitro. 2012;26(2):238–51.

    Article  Google Scholar 

  21. Rosado-Berrios CA, Vélez C, Zayas B. Mitochondrial permeability and toxicity of diethylhexyl and monoethylhexyl phthalates on TK6 human lymphoblasts cells. Toxicol In Vitro. 2011;25(8):2010–6.

    Article  Google Scholar 

  22. Bhattacharya K, Naha PC, Naydenova I, Mintova S, Byrne HJ. Reactive oxygen species mediated DNA damage in human lung alveolar epithelial (A549) cells from exposure to non-cytotoxic MFI-type zeolite nanoparticles. Toxicol Lett. 2012;215(3):151–60.

    Article  Google Scholar 

  23. Liao D, Wu G, Liao B. Zeta potential of shape-controlled TiO2 nanoparticles with surfactants. Colloids Surf A: Physicochem Eng Asp. 2009;348(1):270–5.

    Article  Google Scholar 

  24. Hebeish A, El-Shafei A, Sharaf S, Zaghloul S. Novel precursors for green synthesis and application of silver nanoparticles in the realm of cotton finishing. Carbohydr Polym. 2011;84(1):605–13.

    Article  Google Scholar 

  25. Abou-Okeil A, Amr A, Abdel-Mohdy F. Investigation of silver nanoparticles synthesis using aminated β-cyclodextrin. Carbohydr Polym. 2012;89(1):1–6.

    Article  Google Scholar 

  26. Ansari MA, Khan HM, Khan AA, Cameotra SS, Saquib Q, Musarrat J. Gum arabic capped-silver nanoparticles inhibit biofilm formation by multi-drug resistant strains of Pseudomonas aeruginosa. J Basic Microbiol. 2014;54:688–99.

    Article  Google Scholar 

  27. Kalishwaralal K, BarathManiKanth S, Pandian SRK, Deepak V, Gurunathan S. Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloids Surf B: Biointerfaces. 2010;79(2):340–4.

    Article  Google Scholar 

  28. Piao MJ, Kang KA, Lee IK, Kim HS, Kim S, Choi JY, Choi J, Hyun JW. Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol Lett. 2011;201(1):92–100.

    Article  Google Scholar 

  29. Wei D, Sun W, Qian W, Ye Y, Ma X. The synthesis of chitosan-based silver nanoparticles and their antibacterial activity. Carbohydr Res. 2009;344(17):2375–82.

    Article  Google Scholar 

  30. Solaini G, Sgarbi G, Lenaz G, Baracca A. Evaluating mitochondrial membrane potential in cells. Biosci Rep. 2007;27:11–21.

    Article  Google Scholar 

  31. Li P-W, Kuo T-H, Chang J-H, Yeh J-M, Chan W-H. Induction of cytotoxicity and apoptosis in mouse blastocysts by silver nanoparticles. Toxicol Lett. 2010;197(2):82–7.

    Article  Google Scholar 

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Acknowledgments

The authors would like to gratefully acknowledge Ms. Ashley Allen and Ms. Anne Shanahan for their technical assistance and Dublin Institute of Technology (Dublin, Ireland) for funding under the ABBEST scholarship and Fiosraigh research scholarship program.

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Authors and Affiliations

  1. School of Biological Sciences, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland

    Swarna Jaiswal & Patrick McHale

  2. Centre for Research in Engineering Surface Technology (CREST), FOCAS Institute, Dublin Institute of Technology, Camden Row, Dublin 8, Ireland

    Swarna Jaiswal & Brendan Duffy

  3. Nanolab Research Centre, FOCAS Institute, Dublin Institute of Technology, Dublin, Ireland

    Kunal Bhattacharya

  4. Institute of Environmental Medicine, Karolinska Institute, Nobels Vag 13, Stockholm, Sweden

    Kunal Bhattacharya

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  1. Swarna Jaiswal
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  2. Kunal Bhattacharya
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  3. Patrick McHale
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  4. Brendan Duffy
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Correspondence to Swarna Jaiswal.

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Jaiswal, S., Bhattacharya, K., McHale, P. et al. Dual effects of β-cyclodextrin-stabilised silver nanoparticles: enhanced biofilm inhibition and reduced cytotoxicity. J Mater Sci: Mater Med 26, 52 (2015). https://doi.org/10.1007/s10856-014-5367-1

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  • DOI: https://doi.org/10.1007/s10856-014-5367-1

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