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Tissue response and biodegradation of composite scaffolds prepared from Thai silk fibroin, gelatin and hydroxyapatite

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Abstract

This work aimed to investigate tissue responses and biodegradation, both in vitro and in vivo, of four types of Bombyx mori Thai silk fibroin based-scaffolds. Thai silk fibroin (SF), conjugated gelatin/Thai silk fibroin (CGSF), hydroxyapatite/Thai silk fibroin (SF4), and hydroxyapatite/conjugated gelatin/Thai silk fibroin (CGSF4) scaffolds were fabricated using salt-porogen leaching, dehydrothermal/chemical crosslinking and an alternate soaking technique for mineralization. In vitro biodegradation in collagenase showed that CGSF scaffolds had the slowest biodegradability, due to the double crosslinking by dehydrothermal and chemical treatments. The hydroxyapatite deposited from alternate soaking separated from the surface of the protein scaffolds when immersed in collagenase. From in vivo biodegradation studies, all scaffolds could still be observed after 12 weeks of implantation in subcutaneous tissue of Wistar rats and also following ISO10993-6: Biological evaluation of medical devices. At 2 and 4 weeks of implantation the four types of Thai silk fibroin based-scaffolds were classified as “non-irritant” to “slight-irritant”, compared to Gelfoam® (control samples). These natural Thai silk fibroin-based scaffolds may provide suitable biomaterials for clinical applications.

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Acknowledgments

The financial supports from the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund), Chulalongkorn University Centenary Academic Development Project and National Research Council of Thailand are highly acknowledged. H.T. also thanks Polymer Engineering Laboratory, Biomedical Engineering Laboratory (Faculty of Engineering), and i-Tissue Laboratory (Faculty of Medicine), Chulalongkorn University for the support of laboratory facilities.

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Authors and Affiliations

  1. Biomedical Program, Graduate School, Chulalongkorn University, Phayathai Road, Bangkok, 10330, Thailand

    Hathairat Tungtasana & Siriporn Damrongsakkul

  2. Department of Medical Services, Institute of Pathology, Ministry of Public Health, Yothi Road, Bangkok, 10400, Thailand

    Somruetai Shuangshoti

  3. Department of Pathology and Chulalongkorn GenePRO Center, Faculty of Medicine, Chulalongkorn University, Rama IV Road, Bangkok, 10330, Thailand

    Shanop Shuangshoti

  4. Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Phayathai Road, Bangkok, 10330, Thailand

    Sorada Kanokpanont & Siriporn Damrongsakkul

  5. Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA, 02155, USA

    David L. Kaplan

  6. Department of Otolaryngology, Faculty of Medicine, Chulalongkorn University, Rama IV Road, Bangkok, 10330, Thailand

    Tanom Bunaprasert

Authors
  1. Hathairat Tungtasana
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  2. Somruetai Shuangshoti
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  3. Shanop Shuangshoti
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  4. Sorada Kanokpanont
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  5. David L. Kaplan
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  6. Tanom Bunaprasert
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  7. Siriporn Damrongsakkul
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Correspondence to Siriporn Damrongsakkul.

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Tungtasana, H., Shuangshoti, S., Shuangshoti, S. et al. Tissue response and biodegradation of composite scaffolds prepared from Thai silk fibroin, gelatin and hydroxyapatite. J Mater Sci: Mater Med 21, 3151–3162 (2010). https://doi.org/10.1007/s10856-010-4159-5

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  • DOI: https://doi.org/10.1007/s10856-010-4159-5

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