Abstract
The interaction between L929 cells and carboxymethylchitosan (CM-chitosan)-based hydrogels, hydrogels from pure CM-chitosan and its blends, was examined in this study. Cytotoxicity of all materials was also assessed. The cellular morphology and behavior on the surfaces of the hydrogels were observed by scanning electron microscopy (SEM). The effects of various parameters, e.g., type and content of blended polymers, surface structure of hydrogels, and steaming condition used for the preparation of the hydrogels, on the cell-material response were investigated. The results of the cytotoxicity test revealed that all hydrogels were non-cytotoxic. The SEM micrographs demonstrated that the cells proliferated and spread onto a porous CM-chitosan sample. Better cell spreading was found on a flat surface of a CM-chitosan film. Rounded cells were observed when poly(vinyl alcohol) (PVA) was incorporated into CM-chitosan. Fewer cells were found when the content of PVA increased. Spherical clusters of the aggregated cells existed in the blends with ultra high viscosity carboxymethylcellulose (CM-cellulose). In contrast, with the use of low viscosity CM-cellulose, the cells appeared more spreading. The attached cells on the CM-chitosan film steamed at the highest temperature and longest period appeared to spread the most among all tested steaming conditions.
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C. J. KIRKPATRICK, F. BITTINGER, M. WAGNER, H. KOHLER, T. G. VAN KOOTEN, C. L. KLEIN and M. Otto, Proc. Instn. Mech. Engr. 212 (Part H) (1998) 75
B. D. RATNER, A. S. HOFFMAN, F. J. SCHOEN and J. E. LEMONS, in “Biomaterial Science: Introduction to Materials in Medicine” (Academic Press, New York, 1996) p. 215
T. GROTH and G. ALTANKOV, Biomaterials 17 (1996) 1227
T. KOYANO, N. MINOURA, M. NAGURA and K. I. KOBAYASHI, J. Biomed. Mater. Res. 39 (1998) 486
S. TANODEKAEW, M. PRASITSILP, S. SWASDISON, B. THAVORNYUTIKARN, T. POTHSREE and R. PATEEPASEN, Biomaterials 25 (2004) 1453
E. T. DEN BRABER, J. E. DE RUIJTER, L. A. GINSEL, A. F. VON RECUM and J. A. JANSEN, Biomaterials 17 (1996) 2037
A. CURTIS and C. WILKINSON, Biomaterials 18 (1997) 1573
R. A. A. Muzzarelli, in “Chitin” (Pergamon Press, New York, 1977)
G. I. HOWLING, P. W. DETTMAR, P. A. GODDARD, F. C. HAMPSON, M. DORNISH and E. J. WOOD, Biomaterials. 22 (2001) 2959
R. A. A. MUZZARELLI, Carbohyd. Polym. 8 (1988) 1
X. F. LIU, Y. L. GUAN, D. Z. YANG, Z. LI and K. D. YAO, J. Appl. Polym. Sci. 79 (2001) 1324
X.- G. CHEN, Z. WANG, W.-S. LIU and H.-J. PARK, Biomaterials. 23 (2002) 4609
W. JANVIKUL and B. THAVORNYUTIKARN, J. Appl. Polym. Sci. 90 (2003) 4016
R. I. Freshney, in “Culture of animal cells : a manual of basic technique” (4th edn, A John Wiley & Sons, 1994)
G. B. SCHNEIDER, A. ENGLISH, M. ABRAHAM, R. ZAHARIAS, C. STANFORD and J. KELLER, Biomaterials 25 (2004) 3023
S. F. ROSE, A. L. LEWIS, G. W. HANLON and A. W. LLOYD, Biomaterials 25 (2004) 5125
N. MINOURA, T. KOYANO, N. KOSHIZAKI, H. UMEHARA, M. NAGURA and K.-J. KOBAYASHI, Mat. Sci. Eng. C-Bio. S. 6 (1998) 275
W.-Y. CHUANG, T.-H. YOUNG, C.-H. YAO and W.-Y. CHIU, Biomaterials 20 (1999) 1479
S. VERRIER, R. BAREILLE, A. ROVIRA, M. DARD and J. AMEDEE, J. Mater. Sci-Mater M 7 (1996) 46
Acknowledgments
The entire study was financially supported by National Metal and Materials Technology Center, Thailand. The authors would like to express their thanks to the Animal Cell Culture Lab of The National Center for Genetic Engineering and Biotechnology (BIOTEC) and Ramathibody Hospital, Thailand, for the use of their cell culture facility and the ethylene oxide sterilization, respectively.
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Janvikul, W., Uppanan, P., Thavornyutikarn, B. et al. Fibroblast interaction with carboxymethylchitosan-based hydrogels. J Mater Sci: Mater Med 18, 943–949 (2007). https://doi.org/10.1007/s10856-006-0099-5
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DOI: https://doi.org/10.1007/s10856-006-0099-5