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In vivo assessment of hydroxyapatite and silicate-substituted hydroxyapatite granules using an ovine defect model

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Abstract

Phase pure hydroxyapatite (HA) and two silicate-substituted hydroxyapatites (0.8 and 1.5 wt% Si, or 2.6 and 4.9 wt% SiO4) were prepared by aqueous precipitation methods. The filter-cakes of HA and silicate-substituted hydroxyapatite (SiHA) compositions were processed into granules 1.0–2.0 mm in diameter and sintered at 1200°C for 2 h. The sintered granules underwent full structural characterisation, prior to assessment in an ovine defect model by implantation for a period of 6 and 12 weeks. The results indicate that HA and SiHA implants were well accepted by the host tissue, with no evidence of inflammation. New bone formation was observed directly on the surfaces and in the spaces between the granular implants. Quantitative histomorphometry as determined by the percentage of bone ingrowth and bone coverage for both SiHA implant compositions was significantly greater than that for phase pure HA. These findings indicate that the in vivo bioactivity of hydroxyapatite was significantly improved by the incorporation of silicate ions into the HA structure, making SiHA ceramics attractive alternatives to conventional HA materials for use as bone graft substitute ceramics.

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Authors and Affiliations

  1. Department of Materials Science and Metallurgy, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3QZ, UK

    N. Patel, S. M. Best & W. Bonfield

  2. Orthopaedic Research Unit, University of Cambridge, Box 180, Addenbrooke’s Hospital, Hills Road, Cambridge, CB2 2QQ, UK

    R. A. Brooks, M. T. Clarke, P. M. T. Lee & N. Rushton

  3. School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Fosterhill, Aberdeen, AB25 2ZD, UK

    I. R. Gibson

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  1. N. Patel
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  2. R. A. Brooks
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  3. M. T. Clarke
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Correspondence to S. M. Best.

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Patel, N., Brooks, R.A., Clarke, M.T. et al. In vivo assessment of hydroxyapatite and silicate-substituted hydroxyapatite granules using an ovine defect model. J Mater Sci: Mater Med 16, 429–440 (2005). https://doi.org/10.1007/s10856-005-6983-6

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  • DOI: https://doi.org/10.1007/s10856-005-6983-6

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