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Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles

  • Animal Experimentation
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To evaluate the developmental potential and aneuploidy rates of in-vitro versus in-vivo grown and matured mouse oocytes.

Methods

Mice were superovulated to obtain in-vivo matured oocytes. Mouse preantral follicles were also mechanically isolated and cultured in-vitro. In-vitro fertilization (IVF) was performed and fertilization, cleavage, and morula/blastocyst formation rates were compared between groups. Cytogenetic analysis was used to compare oocyte aneuploidy rates and aneuploidy characteristics in the developing embryos.

Results

In-vivo oocyte maturation resulted in higher IVF fertilization, cleavage, and morula/blastocyst formation rates versus in-vitro follicle culture (96.4% versus 78.5%, p < 0.001; 95.3% versus 77.4%, p < 0.001; 94.1% versus 76.9%, p < 0.001). Total aneuploidy rates were higher in embryos derived from in-vitro matured oocytes versus those grown in-vivo (4.0% versus 1.3%, p < 0.05).

Conclusions

Results indicate a reduced developmental competency of in-vitro matured oocytes. The data also highlight an increased susceptibility to meiotic errors in early stage follicles undergoing in vitro culture.

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Author information

Authors and Affiliations

  1. Andrology and IVF Laboratories, University of Utah School of Medicine, 675 Arapeen Dr. Ste 205, Salt Lake City, UT, 84117, USA

    Lihua Liu, Vincent W. Aoki & Douglas T. Carrell

  2. Department of Surgery (Urology), University of Utah School of Medicine, Salt Lake City, UT, 84108, USA

    Lihua Liu, Vincent W. Aoki & Douglas T. Carrell

  3. Department of Physiology, University of Utah School of Medicine, Salt Lake City, UT, 84108, USA

    Douglas T. Carrell

  4. Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City, UT, 84108, USA

    Douglas T. Carrell

Authors
  1. Lihua Liu
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  2. Vincent W. Aoki
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  3. Douglas T. Carrell
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Corresponding author

Correspondence to Douglas T. Carrell.

Additional information

Capsule Impaired developmental capacity and errors in proper chromosome segregation are observed in mouse oocytes generated from in-vitro preantral follicle culture versus those developed in-vivo.

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Liu, L., Aoki, V.W. & Carrell, D.T. Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles. J Assist Reprod Genet 25, 107–113 (2008). https://doi.org/10.1007/s10815-008-9201-x

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  • DOI: https://doi.org/10.1007/s10815-008-9201-x

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